Endothelial Cell Growth Supplement (ECGS) - BioReagent, for cell culture, sterile, from bovine neural tissue

Cat. No.: E767638

GRADE & PURITY BioReagent, for Cell culture, Sterile, from bovine neural tissue

Synonyms

ECGS

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Overview

Endothelial Cell Growth Supplement (ECGS) is a bovine neural tissue extract containing growth factors for mammalian vascular endothelial cells, which can promote the proliferation of Human Umbilical Vein Endothelial Cells (HUVECs). In combination with interleukin (IL-1), ECGS mediates matrix remodeling in Vascular Smooth Muscle Cells (VSMCs). As a cell culture supplement, it also exerts positive effects on the fusion and growth of hybridoma cells for monoclonal antibody production.

Instructions for Use:

ECGS is prepared by modifying the method established by Maciag et al. It is supplied as a lyophilized powder from a sterile solution containing sodium chloride and streptomycin sulfate. Each vial contains 15 mg of total protein. For reconstitution: Add 0.5 mL of sterile PBS or double-distilled water to the vial and mix gently until fully dissolved to prepare a stock solution at 30 mg/mL. Further dilute the stock solution in sterile tissue culture medium to the desired working concentration. The optimal concentration for vascular endothelial cell growth ranges from 10 to 300 μg/mL.

Precautions:

1. After receiving the product, reconstitute it, aliquot into small portions, and store at -20 °C for up to 6 months, or at 2–8 °C for no more than one week. Avoid repeated freeze-thaw cycles.

2. This product is intended for research use only by professional researchers. It shall not be used for clinical diagnosis, treatment, food production or other purposes.

3. Wear a lab coat and disposable gloves throughout the operation for personal safety and health protection.

Specifications

Synonyms

ECGS

Specifications & Purity

BioReagent, for cell culture, sterile, from bovine neural tissue

Stability And Storage

Store at 2-8℃ short term (1 week). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. Store in the dark.

Storage

Protected from light, Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for Cell culture, Sterile

Chemical and Physical Properties

• Sensitivity: Light-sensitive