Endothelial Cell Growth Supplement (ECGS) - BioReagent, for cell culture, sterile, from bovine neural tissue

Cat. No.: E767638

GRADE & PURITY BioReagent, for Cell culture, Sterile, from bovine neural tissue

Synonyms

ECGS

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Panoramica

Endothelial Cell Growth Supplement (ECGS) is a bovine neural tissue extract containing growth factors for mammalian vascular endothelial cells, which can promote the proliferation of Human Umbilical Vein Endothelial Cells (HUVECs). In combination with interleukin (IL-1), ECGS mediates matrix remodeling in Vascular Smooth Muscle Cells (VSMCs). As a cell culture supplement, it also exerts positive effects on the fusion and growth of hybridoma cells for monoclonal antibody production.

Instructions for Use:

ECGS is prepared by modifying the method established by Maciag et al. It is supplied as a lyophilized powder from a sterile solution containing sodium chloride and streptomycin sulfate. Each vial contains 15 mg of total protein. For reconstitution: Add 0.5 mL of sterile PBS or double-distilled water to the vial and mix gently until fully dissolved to prepare a stock solution at 30 mg/mL. Further dilute the stock solution in sterile tissue culture medium to the desired working concentration. The optimal concentration for vascular endothelial cell growth ranges from 10 to 300 μg/mL.

Precautions:

1. After receiving the product, reconstitute it, aliquot into small portions, and store at -20 °C for up to 6 months, or at 2–8 °C for no more than one week. Avoid repeated freeze-thaw cycles.

2. This product is intended for research use only by professional researchers. It shall not be used for clinical diagnosis, treatment, food production or other purposes.

3. Wear a lab coat and disposable gloves throughout the operation for personal safety and health protection.

Specifications

Sinonimi

ECGS

Specifiche e purezza

BioReagent, for cell culture, sterile, from bovine neural tissue

Stabilità e conservazione

Store at 2-8℃ short term (1 week). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. Store in the dark.

Condizioni di conservazione di stoccaggio

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

BioReagent, per la coltura cellulare, Sterili

Product Line

Prodotti biologici

Proprietà chimiche e fisiche

• Sensibilità: Light-sensitive

Domande frequenti

How should this product be stored?

Store at ?20 °C, protected from light. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does for cell culture mean?

for cell culture indicates that the product has been processed and tested for cell culture and tissue engineering. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.