ENO1 Mouse mAb - Primary antibody, specific to ENO1, Mouse IgG1

Cat. No.: Ab101688
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. See COA
Application
IHC, WB
Host species
Mouse
Reactive species
Cow, Human, Mouse, Rat
Isotype
Mouse IgG1
Clone Number
C2
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab101688-10μl
1
$99.90
50μl
Ab101688-50μl
4
$259.90
100μl
Ab101688-100μl
3
$419.90
1ml
Ab101688-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$2,799.90
Enter a quantity for the sizes you want to add.
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Why this grade

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human ENO1 Antibody, Monoclonal (C2), could be used for WB, IHC and so on. Application WB: 0.01-2 µg/mL IHC: 5-20μg/mL

Protein Function

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Specifications

Product Name
ENO1 Mouse mAb - Primary antibody, specific to ENO1, Mouse IgG1
Synonyms
2 phospho D glycerate hydro lyase antibody | 2-phospho-D-glycerate hydro-lyase antibody | Alpha enolase antibody | Alpha enolase like 1 antibody | Alpha-enolase antibody | C myc promoter binding protein antibody | C-myc promoter-binding protein antibody |
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species
Mouse
Specificity
ENO1
Immunogen
Recombinant ENO1 expressed in E.coli (AA 1-434)
Positive Control
WB: HeLa, MCF7, A549, THP-1, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human kidney tissue.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

ENO1 Mouse mAb (Ab101688) - Western Blot
All lanes: ENO1 Mouse mAb (Ab101688) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 47 kDa
Observed band size: 48 kDa
Exposure time: 2.5 s

ENO1 Mouse mAb (Ab101688) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL ENO1 Mouse mAb (Ab101688)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

ENO1 Mouse mAb (Ab101688) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL ENO1 Mouse mAb (Ab101688)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Application
ApplicationDilution info
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
ENO1 Tchem Alpha-enolase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101820Certificate of AnalysisNov 27, 2023 Ab101688
ZJ23F1101817Certificate of AnalysisNov 27, 2023 Ab101688
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →

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