ENPP1/PC1 Mouse mAb - Anticorpo primario, specific to ENPP1/PC1

Cat. No.: Ab101725

GRADE & PURITY Validated, ExactAb™, See COA

Application

WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

OTI8C7

Conjugation

Unconjugated

Panoramica

Mouse anti Human ENPP1/PC1 Antibody, Monoclonal (OTI8C7), could be used for WB and so on.

Specifications

Product Name

ENPP1/PC1 Mouse mAb - Anticorpo primario, specific to ENPP1/PC1

Sinonimi

M6S1 | NPP1 | NPPS | PC-1 | PCA1 | ARHR2 | COLED | PDNP1 | ectonucleotide pyrophosphatase/phosphodiesterase 1 | E-NPP 1 | ENPP1 | ENPP-1 | Ly-41 Antigen | M6S1NPP1 | Membrane component chromosome 6 surface marker 1 | membrane component, chromosome 6, surf

Specifiche e purezza

ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphospha

Specie ospite

Mouse

Specificità

ENPP1/PC1

Immunogeno

Recombinant protein fragment of human ENPP1 produced in E.coli

Controllo positivo

WB: MCF7 and Hep G2 cell lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, Validated

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: ENPP1/PC1 Mouse mAb (Ab101725)

ENPP1/PC1 Mouse mAb (Ab101725) - Western Blot
All lanes: ENPP1/PC1 Mouse mAb (Ab101725) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 100 kDa
Observed band size: 134 kDa
Exposure time: 100.0 s

Western Blot: ENPP1/PC1 Mouse mAb (Ab101725)

ENPP1/PC1 Mouse mAb (Ab101725) - Western Blot
All lanes: ENPP1/PC1 Mouse mAb (Ab101725) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 100 kDa
Observed band size: 131 kDa
Exposure time: 91.1 s

Applicazione

• Applicazione: Dilution info
• WB:

  1/500-1/2000;

Obiettivi associati (umani)

ENPP1 Tchem Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (0 Activities)

Discover Target: ENPP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

No manufacturer-validated protocols or recommended dilutions are provided in the Product Data for this SKU. Refer to the CoA/Spec Sheet for item-specific instructions. The following outlines general workflows without implying validation for this product:

• Western blot (general)

  • Prepare lysates with protease inhibitors. Resolve by SDS-PAGE, transfer to PVDF/nitrocellulose, block, incubate with primary antibody, wash, and detect with species-appropriate secondary.

• Immunohistochemistry/Immunofluorescence (general)

  • Fix tissue/cells, perform antigen retrieval as required, block non-specific sites, incubate with primary antibody, wash, apply fluorophore- or enzyme-conjugated secondary, counterstain, and mount.

• Flow cytometry (general)

  • Prepare single-cell suspensions, block Fc receptors, stain with primary (and secondary if needed), wash, and analyze. For surface epitopes, avoid permeabilization unless required.

• Immunoprecipitation (general)

  • Couple antibody to Protein A/G resin, incubate with lysate, wash under mild conditions, and elute complexes for analysis.

Important: Optimize dilutions, incubation times, and buffer compositions empirically for your samples. Use appropriate positive/negative controls and secondary reagents matching the mouse primary.

Biological Roles

• Target overview (ENPP1/PC1; general biology)

  • ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1) is a type II transmembrane glycoprotein with an extracellular catalytic domain that hydrolyzes nucleotides such as ATP to generate AMP and pyrophosphate (PPi) (literature). It belongs to the ENPP family implicated in extracellular nucleotide metabolism.

• Functional roles (literature)

  • Regulates extracellular PPi/PO4 balance, influencing mineral deposition processes in the extracellular matrix.
  • Modulates purinergic signaling by controlling availability of nucleotide ligands for P2 receptors.
  • Participates in nucleotide pyrophosphatase/phosphodiesterase activities that can affect ecto-enzymatic networks at the cell surface.

• Experimental implications

  • Antibodies to ENPP1 are useful for probing cell-surface expression, monitoring changes in nucleotide metabolism at the membrane, and characterizing tissue distribution.
  • Because ENPP1 is glycosylated and membrane-associated, epitope accessibility can depend on cell state, fixation, and membrane integrity; assay design should reflect the extracellular location of the catalytic domain.

• Notes and limitations

  • The above describes general biology of ENPP1 and not performance characteristics of this specific antibody. No claims are made regarding diagnostic or therapeutic utility. For this SKU’s host reactivity profile and application validation, consult the CoA/Spec Sheet (not specified in Product Data).

Buffer Applications

This SKU is an antibody reagent and not a buffering compound. As such, classical buffer system guidance (pH ranges, buffer capacity) is not applicable.

• Practical note
  • Use standard immunoassay buffers (e.g., PBS or TBS, with or without Tween-20, and appropriate blocking agents) matched to your application. Select pH and ionic strength to maintain antibody stability and optimize antigen binding.
  • For any item-specific formulation details (buffer composition, preservatives), see the CoA/Spec Sheet (not specified here).

Green Alternatives

While “green chemistry” guidance is oriented toward small-molecule synthesis and solvent choices, antibody workflows can likewise be optimized to reduce environmental impact without compromising data quality.

• Formulation and preservatives

  • If the product contains sodium azide (not specified for this item), consider preservative-free formulations when compatible with your storage and biosafety constraints to reduce hazardous waste. Use sterile technique and aliquoting to maintain sterility.

• Buffer and consumable choices

  • Use phosphate-buffered saline (PBS) prepared from concentrated stocks to minimize packaging waste. Choose biodegradable detergents where possible and avoid excessive solvent-based cleaning.
  • Opt for reusable blotting trays and glassware; select low-protein-binding, recyclable plastics when single-use is unavoidable.

• Process efficiency

  • Optimize antibody dilutions and incubation times to reduce reagent consumption while maintaining signal-to-noise.
  • Implement miniaturized formats (e.g., 96-well ELISA, microfluidic westerns) to reduce buffer and sample volumes.

• Shipping and cold-chain

  • Coordinate consolidated shipments and select slower, ambient-tolerant options only when scientifically permissible. For this SKU, cold shipment is specified; maintain required cold-chain but minimize shipments by planning orders.

• Waste management

  • Segregate any azide- or HRP-containing waste streams per institutional guidelines. Use neutral, non-oxidizing disinfectants to reduce corrosive waste generation.

Pharmaceutical Uses

• Regulatory scope

  • For research use only. Not intended for diagnostic, therapeutic, or in vivo applications.

• Contextual information (general, non-clinical)

  • Monoclonal antibodies are widely used as affinity reagents in bioprocess and analytical workflows, including in-process controls, ligand binding assays, and QC tests. When used in such contexts, reagent qualification, traceability, and change control are critical.

• This item

  • There are no pharmacopeial designations or excipient roles specified for this SKU. Any use in regulated environments should be preceded by appropriate risk assessments and review of the CoA/Spec Sheet to confirm identity, purity, and performance attributes specific to the lot.

• Handling for translational research (general)

  • If the antibody will be coupled to solid supports (e.g., affinity columns) or labels (enzymes/fluorophores), ensure the formulation lacks interfering excipients (amine-containing buffers, carrier proteins) or perform buffer exchange prior to conjugation. Item-specific formulation is not provided here.

Physical Properties

As a protein reagent, small-molecule physical constants are not applicable. Item-specific physico-chemical parameters are not provided.

• Item-specific values

  • Appearance/Concentration: Not specified for this item; refer to CoA/Spec Sheet.
  • Extinction coefficient (ε), isoelectric point (pI), and exact molecular mass: Not specified for this item; refer to CoA/Spec Sheet.
  • Formulation components (buffer, stabilizers, preservatives): Not specified for this item; refer to CoA/Spec Sheet.

• General/literature characteristics for mouse monoclonal IgG

  • Approximate molecular mass: ~150 kDa for intact IgG; Fab fragments ~50 kDa; Fc ~50 kDa (literature, varies by isotype and glycosylation).
  • Solubility: Readily soluble in aqueous buffered saline (e.g., PBS, TBS); precipitation can occur near pI or under low ionic strength (literature).
  • Stability: Enhanced by neutral pH (6.8–7.6), moderate ionic strength, cryoprotectants (e.g., 10–50% glycerol) for frozen storage; avoid repeated freeze/thaw (literature).
  • Temperature sensitivity: Denatures with prolonged exposure to >37°C, extremes of pH, organic solvents, or strong oxidants (literature).

• Practical implications

  • Handle on ice or at 2–8°C during setup. Minimize surface adsorption by using low-protein-binding plastics and including carrier protein (e.g., BSA) when compatible.
  • For concentration determination, use A280 with an appropriate extinction coefficient for the specific isotype and formulation; verify blank/buffer composition prior to measurement.

Quality and Grades

• Supplied grade

  • Grade/Purity: ExactAb™, Validated (as listed in Product Data).

• What this typically means in practice

  • ExactAb™ (Aladdin designation): Internal quality framework emphasizing identity verification, performance consistency, and documentation traceability for antibody reagents. Scope generally includes clone identity control, lot-to-lot bridging, and stability monitoring. Specific acceptance criteria for this SKU are not specified; refer to CoA/Spec Sheet.
  • Validated: Indicates the antibody has undergone functional verification against its intended target using relevant assay modalities. The exact validation assays, sample types, and performance metrics for this item are not specified; consult the CoA/Spec Sheet for details.

• Implications for users

  • Expect consistent performance across lots within the constraints of the ExactAb™ program. Nevertheless, assay conditions (sample prep, antigen retrieval, secondary reagents) can significantly impact outcomes—perform small-scale pilot tests when transferring methods or changing lots.
  • If your workflow is sensitive to background (e.g., IF or IHC), request or review additional documents (CoA, validation images, cross-reactivity screens) before committing critical samples.

• Stabilizers and preservatives

  • Presence/absence and exact concentrations of BSA, glycerol, trehalose, or sodium azide are not specified for this item; refer to CoA/Spec Sheet. These excipients influence storage stability, freezing behavior, and compatibility with live-cell or conjugation workflows.

Reaction and Applications

As an analytical/affinity reagent, this monoclonal antibody is used to detect or capture ENPP1/PC1 rather than to participate in chemical reactions. Typical application families are provided as general guidance; specific validated applications for this SKU are not listed in the Product Data.

• General use cases for monoclonal antibodies against membrane/ectoenzymes

  • Western blot (WB): Detection of denatured ENPP1 under reducing/non-reducing conditions following SDS-PAGE. Epitope preservation depends on antigen domain targeting; optimize transfer and blocking.
  • Immunohistochemistry (IHC): Detection in fixed tissues. Antigen retrieval (heat or enzymatic) may be required depending on epitope masking by fixation/crosslinking.
  • Immunofluorescence (IF): Visualization of cellular/extracellular ENPP1; permeabilization conditions should reflect epitope location (extracellular vs intracellular domains).
  • Flow cytometry (FC): Surface staining of live or fixed cells expressing ENPP1; avoid sodium azide and use Fc-blocking where necessary.
  • Immunoprecipitation (IP)/Pull-down: Enrichment of ENPP1 complexes from lysates; employ protease inhibitors and gentle lysis to preserve surface complexes.
  • ELISA: As capture or detection antibody in sandwich or indirect formats if epitope compatibility exists.

• Practical tips (general)

  • Validate specificity using positive/negative controls (cells or tissues with known ENPP1 expression or genetic knockdown/knockout).
  • Titrate antibody and optimize blocking/detergent conditions to minimize background.
  • For membrane proteins, choose detergents that preserve epitope integrity (e.g., mild non-ionic detergents) and avoid over-fixation.

Reaction Conditions

Classical reaction condition parameters (solvent, temperature, catalysts, yields) do not apply to this antibody reagent. Instead, consider assay condition parameters relevant to immunoassays.

• Assay condition variables (general guidance)

  • Temperature: Perform incubations at room temperature or 4°C depending on assay type and desired stringency. Keep antibody solutions chilled when not actively incubating.
  • Buffer composition: PBS/TBS with optimized salt (100–300 mM) and pH ~7.2–7.6; include mild detergents (e.g., 0.05% Tween-20) for washes to minimize nonspecific interactions when compatible.
  • Blocking: Protein-based blockers (BSA, skim milk, serum) reduce background; select blockers that do not mask epitopes or cross-react with detection reagents.
  • Incubation time: Balance signal with background; longer incubations at lower temperature can improve specificity for some assays.
  • Antigen retrieval/fixation: For IHC, test heat-induced epitope retrieval conditions and fixation chemistries (PFA vs formalin) to preserve the relevant ENPP1 epitope.

• Controls

  • Include positive/negative biological controls, isotype controls, and secondary-only controls to assess specificity and background.

Item-specific, validated application conditions (e.g., tested dilutions, incubation times) are not provided in the Product Data; consult the CoA/Spec Sheet for details.

Safety and Handling

• Regulatory note

  • For research use only. Not for human or veterinary use.

• GHS and hazard information

  • Signal Word: Not specified for this item; refer to SDS.
  • H-Statements: Not specified for this item; refer to SDS.
  • GHS Classification/Pictograms: Not specified for this item; refer to SDS.

• General safety guidance for antibody reagents (defer to SDS for authoritative data)

  • PPE: Laboratory coat, safety glasses, and appropriate gloves (nitrile or latex). Use in a clean area; avoid ingestion and contact with eyes/skin.
  • Handling: Avoid aerosol generation. Open vials carefully to prevent loss by splashing or adsorption on caps/tips. Keep on ice during use to preserve activity.
  • Incompatibilities: Avoid strong oxidizers, proteases, extreme pH, and organic solvents that can denature proteins. Do not mix with biocidal preservatives incompatible with intended downstream use (e.g., sodium azide in live-cell applications).
  • First aid (general): If skin/eye contact occurs, rinse with water for 15 minutes and seek medical advice. If inhaled, move to fresh air. If ingested, rinse mouth and seek medical attention. Provide SDS to responders.

• Waste and decontamination

  • Dispose according to institutional biosafety procedures for non-hazardous biologicals. If sodium azide is present in formulation (not specified here), prevent accumulation in plumbing; collect as hazardous waste.
  • Decontaminate surfaces with appropriate lab disinfectants; avoid harsh oxidants that can damage equipment.

Solvent Selection

This product is an aqueous protein reagent rather than a solvent. Conventional solvent selection guidance does not apply. Instead, consider selection of compatible diluents and wash buffers for immunoassays.

• Compatible diluents (general guidance)

  • PBS or TBS at pH 7.2–7.6 with moderate ionic strength to maintain antibody solubility and stability.
  • Additives to reduce nonspecific binding: 0.05–0.1% Tween-20 in wash buffers; carrier proteins (e.g., 0.1–1% BSA) or serum in blocking/dilution buffers when compatible with the assay.
  • For storage at −20°C, many labs include 10–50% glycerol to minimize freeze–thaw stress; confirm compatibility with your application and the product’s actual formulation.

• When to choose alternatives

  • For antigen–antibody interactions sensitive to salt or detergents, adjust ionic strength or remove surfactants accordingly.
  • For downstream conjugation (e.g., HRP, fluorophores), avoid amine-containing buffers (e.g., Tris) and carrier proteins; use PBS without primary amines, or specialized conjugation buffers.

• Incompatibilities

  • Strong organic solvents, extremes of pH, and chaotropes (e.g., 8 M urea) can denature or unfold antibodies and are not recommended unless part of a controlled elution/refolding protocol.

Storage and Reconstitution

• Supplied storage guidance (from Product Data)

  • Store at −20°C.
  • Avoid repeated freezing and thawing.
  • Shipped in ice chest with ice pads to maintain a cold chain.

• Practical handling recommendations

  • Upon receipt, inspect packaging and immediately transfer to −20°C as directed. If frequent use is expected, prepare single-use aliquots in sterile, low-protein-binding tubes to prevent freeze–thaw cycles.
  • Thaw on ice and mix gently by inversion; avoid vigorous vortexing and foaming which can denature antibodies. Keep on ice during setup.
  • If long-term storage at −80°C or in glycerol-containing buffer is desired, confirm compatibility with the product’s actual formulation (not specified here; refer to CoA/Spec Sheet).

• Reconstitution

  • If received lyophilized (not specified), reconstitute with sterile, filtered buffer (e.g., PBS) at the volume/concentration recommended on the CoA/Spec Sheet. Allow to fully dissolve on ice and mix gently.

• Compatibility notes

  • For applications involving live cells or enzymatic coupling, avoid sodium azide and other biocides in working solutions. The presence/absence of preservatives or carriers in this SKU is not specified; consult documentation.

• Documentation

  • Record lot number, opening date, and aliquot map for traceability. Always refer to the CoA/Spec Sheet for any item-specific storage or reconstitution instructions beyond the guidance above.

Structure and Identity

ENPP1/PC1 Mouse mAb is a monoclonal antibody reagent directed against the ectoenzyme ENPP1 (also known as PC-1). As a protein biologic rather than a small molecule, conventional identifiers (SMILES, InChIKey) do not apply.

• Item-specific identifiers

  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
  • Clone/isotype: Not specified for this item; refer to CoA/Spec Sheet.

• Structural features (general antibody architecture; literature)

  • Monoclonal Ig (mouse), typically composed of two identical heavy chains and two identical light chains, stabilized by inter- and intra-chain disulfide bonds.
  • Y‑shaped 2D topology: Fc region (constant) mediating effector/immobilization interactions; two Fab arms (variable domains) responsible for antigen binding.
  • Variable heavy (VH) and light (VL) domains contain CDR loops that confer specificity to ENPP1 epitopes.

• Antigen context (general)

  • Target: ENPP1/PC1 (ectonucleotide pyrophosphatase/phosphodiesterase 1), a type II transmembrane glycoprotein with an extracellular catalytic domain.

• Notes

  • As a monoclonal antibody preparation, exact sequence, clonality, and any conjugation (if present) are product-specific. None are provided here; consult the CoA/Spec Sheet for definitive identity attributes.

Synthetic Utility

This product is a monoclonal antibody used as a biochemical affinity/analytical reagent and does not serve as a synthetic building block or reagent in classical organic synthesis.

• Not typically applicable

  • It is not employed for bond-forming transformations, catalysis in small-molecule synthesis, or as a protecting group reagent.

• Where it can intersect with synthesis workflows (general)

  • Immunoassays to characterize expression of enzymes or transporters relevant to synthetic biology or biocatalysis projects.
  • Affinity enrichment of protein complexes that may be leveraged in enzyme engineering and pathway optimization.

For small-molecule synthetic planning, refer to reagents specifically designed for transformations, catalysts, or protecting group manipulations.

Target Specificity

• Antigen: ENPP1/PC1 (ectonucleotide pyrophosphatase/phosphodiesterase 1)
• Antibody type: Mouse monoclonal antibody (mAb)
• Epitope/Domain: Not specified for this item; refer to CoA/Spec Sheet.
• Clone ID/Isotype: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity (tested): Not specified for this item; refer to CoA/Spec Sheet.
• Cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Conjugation: Not specified for this item; refer to CoA/Spec Sheet.

Notes

• ENPP1 is a type II transmembrane ectoenzyme with an extracellular catalytic domain; epitope accessibility may depend on fixation and membrane integrity. The precise binding epitope and assay-validated reactivity spectrum for this SKU have not been provided here.