QUALITÀ & PUREZZAMoligand™?Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.≥98%
Moligand™, ≥98% Moligand™ per flussi di lavoro cromatografici e analitici sensibili che richiedono un'interferenza minima della linea di base.
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Stoccaggio e spedizione
Store at -20°C Spedizione Ice chest + Ice pads. Consultare il COA specifico del lotto per le specifiche esatte.
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Documenti di qualità
SDS, COA, scheda tecnica e scheda delle specifiche disponibili per il download. COA specifico del lotto accessibile tramite ricerca per numero di lotto.
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Prova documentale
Citato in 0 pubblicazioni peer-reviewed su cromatografia, sintesi organica e reazioni di accoppiamento incrociato.
Panoramica
Information
ETC-159 ETC-159 (ETC-1922159) is an orally available, potent porcupine inhibitor with an IC50 of 2.9 nM for inhibiting β-catenin reporter activity in STF3A cells.
Targets
Porcn
In vitro
ETC-159 treatment causes decreased abundance of Wnt3a-stabilized β-catenin protein in both mouse L cells and HEK293 cells. ETC-159 inhibits β-catenin signaling in response to multiple active Wnts. ETC-159 inhibits mouse PORCN with an IC50 of 18.1\u2009nM, whereas the IC50 for Xenopus Porcn is approximately fourfold higher (70\u2009nM).
In vivo
ETC-159 is orally bioavailable and effectively inhibits the growth of mouse mammary tumor virus-Wnt1 tumors. After a single oral dose of 5\u2009mg/kg in mice, ETC-159 is rapidly absorbed into the blood with a Tmax of ~0.5\u2009h and oral bioavailability of 100%. The plasma half-life is ~1.18\u2009h and its concentration in the blood remained above the in vitro IC50 for at least 16\u2009h. Treatment of mice with increasing doses of ETC-159 leads to a dose-related increase in exposure. ETC-159 also effectively inhibits Wnt autocrine signaling and growth of teratocarcinomas. ETC-159 effectively inhibits the growth and induces differentiation of colon cancers with RSPO translocations and induces global remodeling of gene expression. Suppression of Wnt/β-catenin signaling with ETC-159 in genetically defined tumors induces irreversible cellular differentiation thus preventing regrowth of these tumors.
ETC-159 (ETC-1922159) is an orally available, potent porcupine inhibitor with an IC50 of 2.9 nM for inhibiting β-catenin reporter activity in STF3A cells.
Condizioni di conservazione di stoccaggio
Store at -20°C
Spedito in
Ice chest + Ice pads
Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.
This compound belongs to the class of organic compounds known as phenylpyridazines. These are organic compounds containing a pyridazine ring substituted by a phenyl group.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
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Recensioni
Recensioni dei clienti
Application Protocols
No item-specific, validated application protocols are provided in the Product Data.
General guidance for small-molecule probe use (non-specification):
Stock solution: Prepare in anhydrous DMSO at a concentration suited to your assay (e.g., 10–50 mM if solubility allows). Aliquot to minimize freeze–thaw cycles and store at −20°C.
Dose–response: Employ serial dilutions (e.g., 1:3 or 1:2) to span several logs of concentration; include vehicle controls matched for DMSO percentage.
Assay controls: Consider orthogonal pathway modulators and negative controls to confirm on-target effects.
Data quality: Monitor compound stability and precipitation throughout the assay; discard any solutions showing visible particulates if they interfere with readouts.
For detailed, application-specific SOPs (e.g., reporter assays, organoid culture workflows), follow your laboratory’s established protocols and consult primary literature describing the use of ETC-159-class probes.
Biological Roles
Context (literature, non-clinical):
ETC-159 is broadly cited as a small-molecule tool compound used to interrogate the Wnt signaling pathway. In numerous studies, chemical probes in this class are employed to modulate upstream processing of Wnt ligands, enabling researchers to assess pathway dependence and downstream effects on transcriptional programs (e.g., TCF/LEF reporter readouts).
Research use scenarios:
Mechanistic studies: Define pathway nodes by perturbing Wnt ligand maturation/transport and measuring proximal biomarkers (e.g., β-catenin stabilization, Wnt target gene expression) under controlled conditions.
Pathway mapping: Time-course and dose–response experiments to quantify sensitivity of diverse cell types to Wnt modulation.
Model systems: Application in 2D cell lines, 3D organoids, or ex vivo systems to evaluate context-specific pathway requirements.
Good practices:
Calibrate concentration ranges empirically and verify effect specificity with orthogonal assays and alternative pathway modulators.
Monitor cellular health (viability, cytotoxicity) alongside desired pathway endpoints to distinguish on-target activity from nonspecific effects.
Disclaimers:
No therapeutic or diagnostic claims are made. Potency, selectivity, and ADME characteristics are assay- and system-dependent and are not specified for this item; consult primary literature and generate laboratory-specific validation data.
Buffer Applications
Not typically applicable. ETC-159 is a bioactive small molecule used as a research probe, not a buffering agent. It does not form defined buffer systems or serve as a pH stabilizer.
Practical guidance (general):
When diluting DMSO stock solutions into aqueous buffers (e.g., PBS, culture media), maintain consistent final solvent percentages across control and treatment groups.
If precipitation occurs upon dilution, adjust the order of addition, include minimal co-solvent, or pre-warm buffers (if compatible) to enhance solubilization.
Filter working solutions if particulates are observed and filtration is compatible with the assay.
For work requiring defined pH environments, pair the compound with a suitable biological buffer (HEPES, MOPS, phosphate) selected based on the assay pH range; the buffer choice is independent of ETC-159.
Green Alternatives
This product is a research small molecule used as a pathway probe, not a bulk solvent or reagent. Therefore, conventional green-chemistry substitution analyses (e.g., replacing chlorinated solvents) are not typically applicable.
General sustainability considerations for handling bioactive small molecules:
Minimize solvent volumes by preparing concentrated stocks and using microplate formats.
Prefer greener solvents for cleaning and processing when compatible (e.g., ethanol or isopropanol instead of acetone/DMF for bench cleanup).
Plan experiments to reduce waste: right-size aliquots to avoid freeze–thaw cycles that lead to disposal of unused material.
Use closed, low-headspace vials to reduce solvent evaporation and emissions.
If large-scale synthesis or formulation development is undertaken (outside the scope of this catalog item):
Consider life-cycle aspects of solvent use, energy for cooling/heating, and waste treatment.
Note: No greener “alternative compound” is offered here because ETC-159 functions as a specific research probe; alternative probes should be selected based on biological mechanism and data quality rather than environmental attributes.
Pharmaceutical Uses
This product is supplied strictly for research use only. No clinical, therapeutic, or diagnostic applications are claimed or supported.
Formulation research context (general):
In preclinical research, small-molecule pathway probes like ETC-159 are often formulated as DMSO stocks for in vitro assays, or in mixed vehicles (e.g., aqueous buffers with co-solvent and/or surfactant) for exploratory in vivo methodology development. Such practices are highly protocol-specific and not specified for this item.
Manufacturing/excipient status:
No pharmacopeial monograph or excipient role is claimed for this item.
Best practices for formulation studies (non-specification):
Characterize solubility across solvent systems and pH; evaluate stability under storage and assay conditions.
Document final vehicle composition and ensure vehicle controls are included in study design.
Compliance note:
Any use beyond basic research (e.g., GLP, IND-enabling studies) requires independent qualification and documentation outside the scope of this product listing.
Physical Properties
Item-specific physico-chemical data:
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Melting point / boiling point: Not specified for this item; refer to CoA/Spec Sheet.
Density, refractive index, logP, pKa: Not specified for this item; refer to CoA/Spec Sheet.
Solubility: Not specified for this item; refer to CoA/Spec Sheet.
General/literature guidance (non-specification):
Bioactive small molecules of this class are commonly formulated as DMSO stock solutions for in vitro use; aqueous solubility often depends strongly on pH and co-solvent content.
If solid, a typical practice is to prepare 10–50 mM DMSO stocks, then dilute into assay buffer with ≤1% final DMSO to minimize solvent effects (verify compound-specific solubility beforehand).
Light and moisture sensitivity can vary; minimize exposure during weighing and solution prep until compound behavior is confirmed.
Practical recommendations (general):
Determine actual solubility empirically in intended assay buffers (with and without serum proteins) and note any precipitation on dilution.
Filter-sterilize working solutions where biologically relevant, using compatible low-binding membranes (PTFE/nylon for organic-rich; PES for aqueous).
Quality and Grades
Item-specific grade:
Grade/Purity: Moligand™ (as provided in Product Data)
Interpretation and context (general):
Moligand™ indicates a small-molecule ligand/compound library designation suitable for discovery and screening workflows. While commonly associated with identity verification and suitability for research assays, the exact acceptance criteria (e.g., chromatographic purity, residual solvent limits, water content, counter-ion content) are not specified here and should be verified in the CoA/Specification Sheet.
What to verify on receipt (best practice):
Identity confirmation: CAS/CID match; optional orthogonal check by LC–MS or HRMS if critical.
Purity profile: Review CoA chromatograms and purity % by the reported analytical method (HPLC/UPLC/GC). If your application is sensitive (e.g., biophysical measurements), consider in-house re-check.
Counterion/salt form: Confirm free base vs. salt and hydrate/solvate state if applicable.
Residual solvents and water: Not specified for this item; consult CoA if these parameters are critical.
Documentation:
Request batch-specific CoA, NMR, LC–MS, and, where available, impurity profiles. Ensure alignment with your internal thresholds for HTS, cell-based assays, or biochemical assays.
Important:
This product is for research use only; not for human or veterinary use. No pharmacopeial grade is claimed unless explicitly stated in the CoA.
Reaction and Applications
This product is positioned as a bioactive research small molecule rather than a synthetic reagent. As such, classical synthetic reaction use (e.g., as reagent, catalyst, or monomer) is not typical.
Research applications (general/literature context):
ETC-159 is widely referenced as a chemical probe in studies interrogating the Wnt signaling pathway. Researchers commonly use such probes to modulate pathway activity in cell-based assays, organoid systems, or biochemical readouts to elucidate pathway dependencies, feedback regulation, and downstream transcriptional consequences.
Practical use guidance (non-specification):
Prepare concentrated DMSO stocks and perform serial dilutions to generate concentration–response curves. Include matched vehicle controls and, where possible, orthogonal readouts (e.g., reporter assays vs. target-proximal biomarkers) to confirm on-target effects.
Validate time-dependence and washout behavior to distinguish primary pathway modulation from secondary effects.
Consider parallel use of mechanistically distinct Wnt-pathway tool compounds to improve interpretability.
Note:
For precise potency, selectivity, and off-target profiles, consult primary literature and validate under your specific assay conditions. No item-specific bioactivity specifications are provided here.
Reaction Conditions
Not applicable in the conventional sense. ETC-159 is not intended for use as a reagent or catalyst, and no synthetic reaction conditions are specified for this item.
General laboratory handling of solutions (non-reaction guidance):
Prepare concentrated stocks in DMSO under clean, dry conditions. Vortex and, if needed, briefly sonicate to fully dissolve. Avoid extended heating unless stability is verified.
For biological assays, dilute into buffer/media with thorough mixing; confirm absence of precipitation and record final solvent percentage.
For researchers conducting chemical modifications or analytical method development around this scaffold, consult peer-reviewed literature for reaction types, conditions, and yields relevant to the authenticated structure; such details are not provided or warranted for this catalog item.
Safety and Handling
Hazard information (item-specific):
GHS classification: Not specified for this item; refer to SDS.
Signal word / H-statements / pictograms: Not specified for this item; refer to SDS.
General laboratory safety (non-SDS, advisory):
Handle in a chemical fume hood and avoid inhalation of dust/aerosols. Wear appropriate PPE: lab coat, safety glasses, and nitrile gloves. For solution preparations, consider double-gloving if using neat DMSO or other strong solvents.
Avoid contact with skin and eyes. Wash thoroughly after handling. Do not eat, drink, or smoke in work area.
Incompatibilities and cautions (general):
Keep away from strong oxidizers and strong acids/bases unless compatibility is known. Avoid prolonged exposure to elevated temperatures or direct light.
When preparing concentrated DMSO stocks, note DMSO’s ability to enhance dermal absorption—clean spills promptly and change contaminated gloves.
First aid overview (consult SDS for authoritative instructions):
Inhalation: Move to fresh air; seek medical attention if symptoms occur.
Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; obtain medical advice if irritation persists.
Ingestion: Rinse mouth; seek medical attention.
Waste management:
Collect solid and solution waste as hazardous organic waste. Decontaminate surfaces with appropriate solvent followed by detergent/water, consistent with institutional EHS guidance.
Note: For definitive hazard classification and exposure limits, always consult the product SDS for this specific lot.
Solvent Selection
Item-specific solubility is not provided; consult the CoA/Spec Sheet. The following guidance is general/literature-based for drug-like small molecules used in pathway biology:
Primary stock preparation (general practice):
DMSO: Typical first-choice solvent due to broad solvating power; prepare 10–50 mM stocks if solubility allows. Store aliquots at −20°C to minimize freeze–thaw.
DMF or ethanol: Alternative organic solvents when DMSO solubility is limited; ensure compatibility with downstream assays.
Assay buffer dilution:
Aqueous buffers (PBS, HBSS, cell culture media) often tolerate ≤0.1–1% v/v DMSO without significant impact on many cell lines/enzymes; verify for your system.
Add compound stock last with vigorous mixing to reduce precipitation. If needed, include small amounts of cosolvent or use serum-containing media that can enhance apparent solubility by binding.
Comparison (general pros/cons):
DMSO: Highest solvency; can affect membrane permeability and assay readouts at higher %.
Ethanol: Volatile and evaporates quickly; limited solubility for very lipophilic compounds.
Aqueous-only: Lowest risk to biology, but solubility may be insufficient without co-solvent or pH adjustment.
Practical tips:
Filter 0.22 µm after dilution if particulates form and filtration is compatible with your assay.
Record final solvent percentage in all conditions (including controls) to ensure interpretability.
Storage and Reconstitution
Item-specific conditions (from Product Data):
Storage: Store at −20°C.
Shipping: Ice chest + ice pads.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
General reconstitution guidance (non-specification):
If supplied as a solid: Equilibrate to room temperature before opening to minimize moisture condensation. Weigh quickly under low humidity if possible. Dissolve in anhydrous DMSO to prepare a concentrated stock. If particulate remains, vortex and briefly sonicate; avoid elevated temperatures unless stability is confirmed.
If supplied in solution: Inspect for precipitation or phase separation upon receipt. Mix gently and, if needed, warm to ambient temperature and vortex to re-dissolve.
Aliquoting and stability:
Prepare single-use aliquots to avoid repeated freeze–thaw. Store tightly sealed, protected from light (amber vials) at −20°C. Record first-open date.
Observe solutions for discoloration or precipitate before use. Discard if significant changes are observed or if past your internal expiry.
Handling notes:
Use dry, compatible solvents and low-binding plasticware or silanized glass to minimize adsorption losses for hydrophobic compounds.
For long-term storage studies or stability-indicating methods, verify integrity by LC–MS/HPLC at defined intervals.
Research use only. Consult the CoA/SDS for any additional, lot-specific handling and storage guidance.
Structure and Identity
Brief description: ETC-159 is a small-molecule research compound widely referenced in the literature as a modulator of the Wnt signaling pathway and used as a chemical probe in pathway biology studies.
Item-specific identifiers (from Product Data):
CAS: 1638250-96-0
CID: 86280523
InChIKey: 306244 (as provided)
Grade: Moligand™
SKU: E413550
Structure descriptors:
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
Structural features (general/literature context, not item-specific):
ETC-159 is cited in the literature as a small-molecule inhibitor targeting lipidation steps in Wnt ligand processing; detailed substructure and stereochemistry should be verified from primary literature or a validated CoA.
2D structure summary (literature-level, qualitative): a drug-like organic molecule containing multiple heteroatoms and aromatic/heteroaromatic fragments typical of kinase/enzyme-pathway modulators; exact ring count, substitution pattern, and stereochemistry are to be confirmed from a validated structure source.
Important note:
For definitive structural identifiers (complete InChIKey, canonical SMILES, exact stereochemical specification), consult the Certificate of Analysis/Specification Sheet for this lot or authoritative databases corresponding to the provided CAS/CID.
Synthetic Utility
Not typically applicable. ETC-159 is provided as a finished small-molecule probe for biological research rather than a building block, reagent, or catalyst for synthetic chemistry.
If synthetic derivatization or SAR studies are planned (general considerations):
Obtain a validated structure and assess functional handles amenable to modification (literature sources). Plan orthogonal protection strategies if needed.
Establish robust analytical methods (UPLC, LC–MS, NMR) for rapid characterization of analogs and impurity profiles.
Because item-specific functional groups and reactivity are not specified here, no reagent-class guidance (e.g., nucleophile/electrophile behavior, named reaction compatibility) is provided. Consult primary literature for the exact scaffold and synthetic approaches if derivative synthesis is intended.
Target Specificity
Item-specific target/assay validation data are not provided in the Product Data for this listing.
Notes:
The “Target Specificity” section in this catalog is typically used for biological macromolecules (e.g., antibodies) with defined antigen/epitope reactivity. For small-molecule research probes, specificity profiles (primary target, off-targets, kinome/panel screening) must be obtained from primary literature or internal validation and are assay-dependent.
No claims are made here regarding potency, selectivity, or target engagement for this specific lot; consult the CoA/Spec Sheet and peer-reviewed sources for guidance before experimental use.
Domande frequenti
What is the purity of this product?
This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.
What is Moligand??
Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.
How should this product be stored?
Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 1638250-96-0, the molecular formula is C19H17N7O3, and the molecular weight is 391.38 g/mol. InChIKey QTRXIFVSTWXRJJ-UHFFFAOYSA-N.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
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