Dissolve 50 grams per liter of ddH2O. Heat with stirring until dissolved. Dispense into appropriate containers, loosen caps and autoclave (15psi) for 15 minutes at 121ºC.
Component
g/liter
Agar
20
Dextrose
2.5
Potassium Phosphate, Dibasic
2.5
Sodium Chloride
5
Papaic Digest of Soybean Meal
3
Pancreatic Digest of Casein
17
Total: 50g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
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