Dissolve 20grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
Pancreatic Digest of Gelatin
10
Sodium Chloride
5
Disodium Phosphate
1.5
Monopotassium Phosphate
3.5
Total: 28g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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