Construction of siRNA expression vector targeting EGFR gene
Laboratory Protocols & SOPs Library
Construction of siRNA expression vector targeting EGFR gene
·Published ·Updated
Summary
The constructed psj-EGFR vector was transfected into A549 cells using Lipofeetamine2000, and real-time quantitative fluorescence PCR, immunofluorescence and flow cytometry were applied to detect the expression of EGFR gene at the mRNA and protein levels, and tetramethyl azole blue colorimetric assay (MTT), and colony formation assay were used to observe the changes in cell growth.
For more product details, please visit Aladdin Scientificwebsite.
Was this article helpful?YesNo19 out 42 found this helpful
Utilizziamo i cookie per garantire il corretto funzionamento del sito web e, ove consentito, per migliorare la tua esperienza. Puoi gestire le tue preferenze in qualsiasi momento nelle Impostazioni. Per saperne di più, consulta la nostra Cookie Policy.
Desidera che le inviamo un messaggio quando avremo sconti disponibili?
Ricordamelo più tardi
Grazie! Controlli la sua casella di posta per confermare.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
