Dissolve 42.5grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized. Do not autoclave.
Component
g/liter
Peptic Digest of Animal Tissue
5
Lactose
10
Casein Digest Peptone
5
Sodium Chloride
5
Sodium Citrate
2
Sodium Deoxycholate
0.5
Neutral Red
0.033
Agar
15
Total: 42.5g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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