Suspend 30g of the powder in 1L of distilled water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
Tryptone
20
Dextrose
10
Bromcresol Purple
Total: 30.04g/Liter
Storage and Stability:
Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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