Dissolve 37grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Component
g/liter
Casein Digest Peptone
15
Deoxyribonucleic Acid
2
Papaic Digest of Soybeans
5
Sodium Chloride
5
Agar
15
Toluidine Blue O
0.1
Total: 37g/Liter
Storage and Stability:
Store powdered media at 4°C Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should stored at 4°C and used within a short period of time.
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