Extraction and activity of ATPase from cardiac myofibrillar fibers
Laboratory Protocols & SOPs Library
Extraction and activity of ATPase from cardiac myofibrillar fibers
·Published ·Updated
Summary
Cardiac myofibrillar ATPase is Ca2+, Mg2+ ATPase, which breaks down ATP into ADP and Pi and releases energy to provide myofibrillar contraction needs. Myofibrils were extracted by Salaro and Pagani method, mainly by differential centrifugation and Triton X-100 stripping. The enzyme activity was determined in much the same way as that of the myoplasmic reticulum Ca2+ATPase.
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Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Aladdin Scientific. "Extraction and activity of ATPase from cardiac myofibrillar fibers" Aladdin Knowledge Base, updated 9 giu 2025. https://www.aladdinsci.com/eu_it/faqs/extraction-and-activity-of-atpase-from-c-en.html
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