In the presence of mouse IL-4 (Mil-4), IL-10 stimulated proliferation of the D36 mouse mast cell line. In contrast, single mIL4 or hIL-10 had only very low proliferation-stimulating activity.
D36 cell suspension preparation①↓Inoculate cells in a 96-well culture plate②.↓ Add the sample to be tested and the standard ③↓plus 3H-TdR④↓ Cells were collected and the amount of 3H-TdR adulteration was determined ⑤↓Plot the standard curve and calculate the activity units of IL-10 of the samples to be tested ⑥
Caveat
①Take the vigorously growing D36 cells and wash them twice with IMDM culture medium, suspend them in 20% FCS-IMDM culture medium, and make a concentration of 2×104/ml.②~⑥ Refer to IL-4 assay.
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