Dissolve 25.94 grams per liter of sterile distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
NZ Amine
10
Casamino Acidse
10
NaCl
5
MgCl2
0.94
Total: 25.94g/Liter
500g makes 19.3 liters
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
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