Recombinant Human ACSL5 Protein: FAQs & Key Facts

Recombinant Human ACSL5 Protein is a research-grade recombinant enzyme representing the human long-chain acyl-CoA synthetase 5 isoform, essential for studying fatty acid activation, lipid metabolism pathways, and related disease mechanisms.

Product Name

Recombinant Human ACSL5 Protein

SKU

rp368016

Gene / Protein Target

ACSL5 (Long-chain acyl-CoA synthetase 5, also known as FACL5)

Protein Function

Catalyzes the conversion of long-chain fatty acids to acyl-CoA thioesters, a key activation step in fatty acid metabolism

Supplier Reference

absea#PP-11397

Intended Use

In vitro research use only; not for diagnostic or therapeutic use

What Is ACSL5 and Why Is It Important in Research?

ACSL5 (Acyl-CoA Synthetase Long-Chain Family Member 5), also historically referred to as FACL5, is a member of the long-chain acyl-CoA synthetase (ACSL) family of enzymes. These enzymes catalyze the ATP-dependent conversion of long-chain fatty acids (typically C12–C20) into their acyl-CoA derivatives — an obligatory first step that channels fatty acids into downstream metabolic pathways including β-oxidation, phospholipid biosynthesis, and triglyceride synthesis.

ACSL5 is particularly expressed in the small intestine and has also been detected in other tissues. Research interest in ACSL5 has grown substantially due to its proposed roles in apoptosis regulation, intestinal lipid absorption, and potentially in cancer biology. Understanding the enzymatic activity and protein–protein interactions of ACSL5 requires access to high-quality recombinant protein, making Recombinant Human ACSL5 Protein a valuable reagent for mechanistic and biochemical studies.

Product Specifications and Quality Overview

Recombinant Human ACSL5 Protein (SKU: rp368016) is offered by Aladdin Scientific as a research-grade reagent. The product references the supplier designation absea#PP-11397. As with all recombinant proteins in Aladdin's catalog, researchers should consult the current product datasheet for the most up-to-date specifications, including molecular weight, expression host, tag information, purity grade, formulation buffer, and concentration.

Key quality attributes to verify on the datasheet include: (1) expression system used (e.g., E. coli, baculovirus/insect cells, HEK293, or CHO cells), which affects post-translational modifications; (2) purity percentage as assessed by SDS-PAGE or HPLC; (3) endotoxin levels relevant for cell-based assays; and (4) activity validation data where available. Always download the latest Certificate of Analysis (CoA) from the Aladdin Scientific website to confirm lot-specific data before experimental use.

Recommended Research Applications

Recombinant Human ACSL5 Protein is suitable for a range of in vitro biochemical and cell biology research applications. Typical uses include:

• Enzymatic activity assays: Measuring acyl-CoA synthetase activity using fatty acid substrates and quantifying CoA thioester formation.
• Protein–protein interaction studies: Pull-down assays, co-immunoprecipitation setups, or surface plasmon resonance (SPR) experiments to identify binding partners of ACSL5.
• Antibody validation: Using the recombinant protein as a positive control or standard for Western blot, ELISA, or immunoassay development targeting ACSL5.
• Structural studies: Providing purified protein for X-ray crystallography, cryo-EM, or biophysical characterization.
• Drug discovery screening: Serving as a target protein in inhibitor or activator screening assays relevant to metabolic disease or oncology research.

This product is strictly for research use only and is not intended for clinical diagnostics, therapeutics, or veterinary use.

Storage, Handling, and Reconstitution Guidelines

Proper handling of Recombinant Human ACSL5 Protein is critical to preserving enzymatic activity and protein integrity. While lot-specific conditions are always detailed in the product datasheet and CoA, the following general best practices apply to recombinant proteins of this class:

• Storage temperature: Recombinant proteins are typically stored at –20 °C for short-term use or –80 °C for long-term storage. Avoid repeated freeze–thaw cycles, as these can cause aggregation and loss of activity.
• Aliquoting: Upon first thaw, divide the protein into single-use aliquots to minimize freeze–thaw events.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer specified on the datasheet (commonly sterile PBS or a carrier protein-containing buffer). Allow the vial to equilibrate to room temperature before opening.
• Working concentration: Determine optimal working concentration empirically for each specific assay format.
• Carrier proteins: For dilute working solutions, adding BSA (0.1–0.5%) or other carrier proteins may help prevent non-specific adsorption to tube surfaces.

Always refer to the current product insert for lot-specific storage instructions. Contact Aladdin Scientific technical support if you have questions specific to your application.

Ordering, Pricing, and Technical Support

Recombinant Human ACSL5 Protein is available from Aladdin Scientific under SKU rp368016. To place an order or request a quotation, visit the Aladdin Scientific website or contact your regional sales representative. The following guidance applies to procurement:

• Quantity options: Please check the current product listing for available pack sizes and concentration options, as these may be updated periodically.
• Pricing: Current pricing is available on the Aladdin Scientific website or upon request from the sales team. Volume discounts may be available for large-quantity orders.
• Lead time: Check product availability status online; some recombinant proteins may be made-to-order with extended lead times.
• Technical support: For application-specific questions, protocol assistance, or troubleshooting, contact Aladdin Scientific's technical support team. Reference SKU rp368016 and lot number when submitting inquiries.
• Documentation: SDS (Safety Data Sheet), CoA, and datasheet are available for download from the product page on the Aladdin Scientific website.

Frequently asked questions

What is the biological function of ACSL5?

ACSL5 (Long-Chain Acyl-CoA Synthetase 5) catalyzes the ATP-dependent ligation of long-chain fatty acids (C12–C20) to coenzyme A, forming acyl-CoA thioesters. This activation step is essential for channeling fatty acids into metabolic pathways such as β-oxidation, phospholipid biosynthesis, and triglyceride synthesis. ACSL5 has also been implicated in the regulation of apoptosis and intestinal lipid absorption.

What expression system is used to produce Recombinant Human ACSL5 Protein (rp368016)?

The specific expression host for SKU rp368016 is detailed in the product datasheet and Certificate of Analysis available on the Aladdin Scientific website. Common expression systems for recombinant ACSL5 include E. coli, baculovirus-infected insect cells, and mammalian cell lines such as HEK293. Please download the current datasheet or contact Aladdin Scientific technical support to confirm the expression system for your specific lot.

What purity level can I expect for Recombinant Human ACSL5 Protein?

Purity specifications for rp368016 are provided in the lot-specific Certificate of Analysis and product datasheet. Research-grade recombinant proteins from Aladdin Scientific are typically assessed by SDS-PAGE and/or HPLC. Please refer to the CoA downloadable from the product page for the exact purity value for your lot.

How should I store Recombinant Human ACSL5 Protein to maintain activity?

In general, recombinant proteins should be stored at –20 °C for short-term use or –80 °C for long-term storage. Repeated freeze–thaw cycles should be avoided; single-use aliquots are strongly recommended. Always follow the specific storage instructions provided in the product datasheet for rp368016, as formulation-specific conditions may differ.

What research applications is Recombinant Human ACSL5 Protein validated for?

Recombinant Human ACSL5 Protein (rp368016) is suitable for in vitro applications including enzymatic activity assays, protein–protein interaction studies, antibody validation (e.g., Western blot, ELISA), structural biology studies, and drug discovery screening. Validated applications for the specific lot are listed in the product datasheet. This product is for research use only.

How do I order Recombinant Human ACSL5 Protein from Aladdin Scientific?

You can order Recombinant Human ACSL5 Protein directly through the Aladdin Scientific website using SKU rp368016, or by contacting your regional Aladdin Scientific sales representative. For bulk orders, custom requests, or pricing inquiries, reach out to the sales team directly. Documentation including SDS, CoA, and datasheet can be downloaded from the product page.

References

1. Fujino T, Kang MJ, Suzuki H, Iijima H, Yamamoto T. Cloning and functional expression of a novel long-chain acyl-CoA synthetase expressed in brain. Journal of Biological Chemistry. 1996;271(28):16748-16752.
2. Muoio DM. Metabolic inflexibility: when mitochondrial indecision leads to metabolic gridlock. Cell. 2014;159(6):1253-1262.
3. Aladdin Scientific. Recombinant Human ACSL5 Protein (rp368016) Product Datasheet. Aladdin Scientific; [current version].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.