Recombinant Human ACTR3C Protein: Key Facts & FAQs

Recombinant Human ACTR3C Protein is a research-grade recombinant protein derived from the human ACTR3C gene, belonging to the actin-related protein family and intended for use in biochemical and cell biological studies of cytoskeletal dynamics.

Product Name

Recombinant Human ACTR3C Protein

SKU

rp369105

Protein Family

Actin-related protein (Arp) family

Species Origin

Human (Homo sapiens)

Supplier Reference

absea#PP-25489

Intended Use

Research use only (RUO)

What Is ACTR3C and Why Is It Relevant to Research?

ACTR3C (Actin-Related Protein 3C), also referred to as Arp3C or ARP3B in some literature, is a member of the actin-related protein (Arp) superfamily. Actin-related proteins share significant sequence and structural homology with conventional actin but serve specialized roles in a variety of cellular processes, including cytoskeletal organization, chromatin remodeling, and intracellular transport.

ACTR3C is thought to participate in Arp2/3 complex-related pathways that regulate actin nucleation and branching, processes that are fundamental to cell motility, endocytosis, and maintenance of cell shape. Research into ACTR3C and related Arp family members is relevant to fields such as cell biology, oncology, and neuroscience, where cytoskeletal dynamics play critical roles.

Note: Detailed functional characterization data specific to ACTR3C should be verified against current peer-reviewed literature, as functional annotation of this paralog is still evolving.

Product Specifications and Quality Attributes

Recombinant Human ACTR3C Protein (SKU: rp369105) is produced recombinantly to support reproducible research outcomes. Key specification attributes typically associated with recombinant proteins of this class include:

• Expression System: The specific expression host (e.g., E. coli, baculovirus/insect cell, HEK293, or wheat germ) should be confirmed from the product datasheet prior to use, as it affects post-translational modification status.
• Purity: Recombinant proteins from Aladdin Scientific are generally supplied at high purity, typically verified by SDS-PAGE. Confirm lot-specific purity from the certificate of analysis (CoA).
• Formulation: The protein is typically supplied in a buffered solution or as a lyophilized powder. Refer to the product datasheet for exact formulation details.
• Tag Information: Some recombinant ACTR3C preparations may include an affinity tag (e.g., His-tag or GST-tag) to facilitate purification and detection; confirm from the datasheet.

Researchers are advised to download the current product datasheet from Aladdin Scientific's website or contact technical support to obtain lot-specific CoA data.

Recommended Applications and Experimental Use

Recombinant Human ACTR3C Protein is intended for research use only and is suited to a range of biochemical and cell biological applications. Common applications for recombinant actin-related proteins of this type include:

• In vitro biochemical assays: Protein–protein interaction studies, pull-down assays, and co-immunoprecipitation experiments to map ACTR3C binding partners.
• Structural studies: Use as an antigen for antibody production or as a reference standard in Western blotting experiments.
• Functional assays: Investigation of actin nucleation and polymerization dynamics in cell-free systems.
• ELISA standards: As a calibration standard for quantitative immunoassays targeting ACTR3C.

Always validate the protein's activity in your specific assay format. Optimization of protein concentration, buffer conditions, and incubation parameters is recommended before committing to large-scale experiments.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human ACTR3C Protein are critical to maintaining protein integrity and biological activity:

• Storage temperature: Recombinant proteins are generally stored at −20 °C or −80 °C for long-term preservation. Confirm the recommended temperature from the product datasheet or CoA.
• Avoid repeated freeze-thaw cycles: Aliquot the protein upon first thaw into single-use volumes to prevent activity loss caused by freeze-thaw degradation.
• Carrier protein: If the protein is supplied without a stabilizing carrier (e.g., BSA), consider adding a suitable carrier to working dilutions to prevent adsorption to tube surfaces.
• Working solutions: Prepare working dilutions fresh and keep on ice during experiments to minimize proteolytic degradation.
• Sterility: Handle under aseptic conditions if the protein is to be used in cell-based assays.

Always refer to the lot-specific CoA and current product datasheet for definitive storage and stability information, as formulation details may vary between production lots.

Ordering, Availability, and Technical Support

Recombinant Human ACTR3C Protein is available from Aladdin Scientific under SKU rp369105. To place an order or inquire about availability:

• Visit the Aladdin Scientific website and search by SKU or product name.
• Contact your regional Aladdin Scientific sales representative for pricing, volume discounts, and lead-time information.
• For technical questions regarding experimental design, compatibility, or troubleshooting, reach out to Aladdin Scientific's technical support team.

When placing your order, have the following information ready: SKU (rp369105), required quantity, shipping address, and any applicable institutional purchase order details. Aladdin Scientific's customer service team can assist with questions related to shipping, payment, and returns in accordance with current company policies.

Frequently asked questions

What is ACTR3C and what is its biological function?

ACTR3C (Actin-Related Protein 3C) is a member of the actin-related protein superfamily, which shares structural homology with conventional actin. It is implicated in cytoskeletal organization, potentially contributing to Arp2/3 complex-associated actin nucleation and branching pathways that regulate cell motility and intracellular transport. Detailed functional characterization of ACTR3C is an active area of research.

What applications is Recombinant Human ACTR3C Protein (rp369105) suitable for?

Recombinant Human ACTR3C Protein is suitable for research applications including in vitro protein–protein interaction assays, pull-down and co-immunoprecipitation studies, Western blot reference standards, ELISA calibration standards, and cell-free functional assays examining actin dynamics. This product is for research use only and is not intended for diagnostic or therapeutic purposes.

How should Recombinant Human ACTR3C Protein be stored to maintain stability?

To maintain protein stability, store Recombinant Human ACTR3C Protein at −20 °C or −80 °C as indicated on the product datasheet. Avoid repeated freeze-thaw cycles by aliquoting the protein into single-use volumes upon first thaw. Always refer to the lot-specific certificate of analysis (CoA) for definitive storage recommendations.

What expression system is used to produce rp369105, and does it affect experimental results?

The specific expression system used for rp369105 should be confirmed from the current product datasheet, as this information may vary. The choice of expression host (prokaryotic vs. eukaryotic) affects post-translational modifications such as glycosylation and phosphorylation, which can influence protein folding, activity, and binding interactions. Researchers should select the appropriate form based on their experimental requirements.

How can I order Recombinant Human ACTR3C Protein from Aladdin Scientific?

You can order Recombinant Human ACTR3C Protein by searching for SKU rp369105 on the Aladdin Scientific website or by contacting your regional sales representative. For bulk orders, custom quantities, or pricing inquiries, please reach out to the Aladdin Scientific sales team directly. Technical support is also available for questions about product suitability and experimental design.

References

1. Machesky, L. M. & Gould, K. L. The Arp2/3 complex: a multifunctional actin organizer. Current Opinion in Cell Biology. (General textbook-level reference for Arp family context.)
2. Pollard, T. D. & Borisy, G. G. Cellular motility driven by assembly and disassembly of actin filaments. Cell. (Foundational reference for actin dynamics research.)
3. Aladdin Scientific. Product Datasheet: Recombinant Human ACTR3C Protein, SKU rp369105. Aladdin Scientific. (Supplier reference; consult current version for lot-specific data.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.