Recombinant Human ATAD2B Protein: FAQs & Key Facts

Recombinant Human ATAD2B Protein is a research-grade recombinant protein derived from the human ATAD2B gene, designed to support investigations into ATPase activity, bromodomain function, and chromatin-remodeling mechanisms in mammalian cells.

Product Name

Recombinant Human ATAD2B Protein

SKU

rp367449

Source Gene

Human ATAD2B (ATPase Family AAA Domain-Containing Protein 2B)

Protein Family

AAA+ ATPase family; contains ATPase and bromodomain regions

Primary Research Applications

Chromatin remodeling studies, ATPase activity assays, bromodomain interaction studies, biochemical assays

Supplier Reference

absea#PP-2922

What Is Recombinant Human ATAD2B Protein?

ATAD2B (ATPase Family AAA Domain-Containing Protein 2B) is a member of the AAA+ ATPase superfamily, which is characterized by conserved ATPase domains that couple ATP hydrolysis to diverse cellular activities. The human ATAD2B protein also contains a bromodomain, a structural module that recognizes acetylated lysine residues on histone tails, placing ATAD2B at the intersection of ATP-dependent chromatin remodeling and epigenetic regulation.

Recombinant Human ATAD2B Protein (SKU: rp367449) is produced using recombinant expression technology to deliver a research-grade protein suitable for in vitro biochemical and cell biology experiments. It is catalogued under supplier reference absea#PP-2922 and is available from Aladdin Scientific for researchers investigating chromatin biology, epigenetics, and related disease mechanisms.

Recommended Applications and Use Cases

Recombinant Human ATAD2B Protein is designed for use in a range of in vitro research applications. Typical uses include:

• ATPase activity assays: Measuring ATP hydrolysis kinetics to characterize enzyme activity and screen potential inhibitors.
• Bromodomain binding studies: Investigating the interaction of ATAD2B's bromodomain with acetylated histone peptides or small molecules, relevant to epigenetic drug discovery.
• Chromatin remodeling assays: Studying the role of ATAD2B in nucleosome dynamics and chromatin organization.
• Protein–protein interaction studies: Using the recombinant protein as bait or prey in pull-down or co-immunoprecipitation experiments.
• Antibody validation: Serving as a positive control antigen for validating ATAD2B-specific antibodies in Western blot or ELISA formats.

Researchers should optimize assay conditions, including buffer composition, protein concentration, and incubation parameters, for their specific experimental system.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins is critical to maintaining activity and integrity. General best-practice recommendations for Recombinant Human ATAD2B Protein include:

• Storage temperature: Store at –80 °C for long-term preservation. Avoid repeated freeze–thaw cycles, which can lead to protein aggregation and loss of activity.
• Aliquoting: Upon receipt, it is recommended to aliquot the protein into single-use volumes to minimize freeze–thaw cycling.
• Working conditions: Keep the protein on ice during experimental setup. Use an appropriate protein-stabilizing buffer as specified in the product datasheet.
• Expiry and quality control: Check the certificate of analysis (CoA) provided with each lot for purity, concentration, and activity data specific to that batch.

Note: Specific storage buffer composition, concentration, purity grade, and stability data should be confirmed with the product datasheet or by contacting Aladdin Scientific technical support, as these details were not fully specified in the available source material.

Ordering and Product Information

Recombinant Human ATAD2B Protein is available from Aladdin Scientific under the following details:

• SKU: rp367449
• Supplier Reference: absea#PP-2922
• Product Name: Recombinant Human ATAD2B Protein

To place an order, request a quote, or obtain a certificate of analysis and safety data sheet, please contact Aladdin Scientific customer service or visit the product page on the Aladdin Scientific website. Bulk quantities, custom formulations, or specific activity requirements may be accommodated upon request — please reach out to the technical sales team for further discussion.

For questions regarding shipping conditions, payment options, returns, or account management, refer to the relevant sections of the Aladdin Scientific Help Center or contact customer support directly.

Frequently asked questions

What is the biological function of ATAD2B?

ATAD2B is a member of the AAA+ ATPase superfamily that contains both an ATPase domain and a bromodomain. The ATPase domain is involved in ATP hydrolysis-coupled cellular processes, while the bromodomain mediates recognition of acetylated lysine residues on histone tails, linking ATAD2B to chromatin remodeling and epigenetic regulation. Its precise physiological roles and disease associations remain an active area of research.

What expression system is used to produce Recombinant Human ATAD2B Protein (rp367449)?

The specific expression system (e.g., E. coli, baculovirus/insect cell, mammalian cell) used to produce Recombinant Human ATAD2B Protein (rp367449) is not detailed in the currently available source material. Please refer to the product datasheet or contact Aladdin Scientific technical support for confirmed expression system, host, and tag information.

How should I store and handle Recombinant Human ATAD2B Protein to preserve its activity?

As a general best practice for recombinant proteins, store Recombinant Human ATAD2B Protein at –80 °C for long-term storage and avoid repeated freeze–thaw cycles by aliquoting into single-use volumes upon receipt. Keep the protein on ice during experimental procedures. Always consult the certificate of analysis (CoA) and product datasheet provided with your specific lot for exact storage buffer, concentration, and stability recommendations.

What are the recommended quality control checks for this protein?

Each lot of Recombinant Human ATAD2B Protein should be accompanied by a certificate of analysis (CoA) confirming purity (typically assessed by SDS-PAGE), protein concentration, and, where applicable, functional activity data. Researchers should verify the CoA upon receipt and may perform additional in-house validation such as Western blot or activity assays before use in critical experiments.

Can Recombinant Human ATAD2B Protein be used for drug discovery or inhibitor screening?

Yes, Recombinant Human ATAD2B Protein is suitable for in vitro drug discovery applications, including ATPase inhibitor screening and bromodomain-targeted small molecule binding assays. Its dual-domain architecture makes it a relevant tool for epigenetic drug discovery programs. Researchers should optimize assay conditions and validate readouts appropriate to their screening platform.

References

1. Aladdin Scientific product page: Recombinant Human ATAD2B Protein, SKU rp367449 (absea#PP-2922).
2. Ciro M, et al. ATAD2 is a novel cofactor for MYC, overexpressed and amplified in a broad range of human tumors. Cancer Research, 2009. [Note: This reference pertains to ATAD2, the paralog of ATAD2B; applicability to ATAD2B should be verified.]
3. UniProt entry for human ATAD2B protein (UniProtKB). [Accession to be confirmed by technical reviewer.]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.