Recombinant Human ATG9A Protein: FAQs & Key Facts

Recombinant Human ATG9A Protein is a key autophagy-related transmembrane protein produced recombinantly for use in biochemical, cell biology, and autophagy pathway research applications.

Product Name

Recombinant Human ATG9A Protein

SKU

rp365042

Gene / Protein Target

ATG9A (Autophagy-related protein 9A)

Protein Origin

Human (Homo sapiens)

Primary Research Area

Autophagy, membrane trafficking, lysosomal biology

Format

Recombinant protein

What Is ATG9A and Why Is It Important in Research?

ATG9A (Autophagy-related protein 9A) is the only multi-spanning transmembrane protein in the core autophagy machinery conserved from yeast to humans. It is encoded by the ATG9A gene and plays a central role in autophagosome biogenesis by trafficking membrane lipids to the forming autophagosome. ATG9A cycles between the trans-Golgi network, endosomes, and the autophagosome assembly site, making it a critical regulator of autophagic flux.

Because of its unique transmembrane nature and its role as a lipid scramblase, ATG9A has emerged as a high-value target for studies of autophagy regulation, neurodegenerative disease, cancer biology, and infection biology. Recombinant Human ATG9A Protein provides researchers with a well-defined tool to study protein–protein interactions, enzyme activity, and pathway regulation in vitro.

Product Specifications and Quality

Recombinant Human ATG9A Protein (SKU: rp365042) is manufactured using recombinant expression technology to deliver a consistent, research-grade reagent. The product is designed for use in assays requiring defined protein concentrations and high batch-to-batch reproducibility.

• Target Protein: ATG9A (Human)
• SKU: rp365042
• Grade: Research reagent
• Application suitability: Biochemical assays, binding studies, autophagy pathway research, antibody validation

For detailed specifications including molecular weight, tag information, expression host, purity percentage, and formulation buffer, please refer to the official product datasheet available on the Aladdin Scientific product page or contact technical support.

Recommended Applications and Usage Guidelines

Recombinant Human ATG9A Protein is suitable for a range of in vitro research applications. Typical uses include:

• Protein interaction studies: Identifying binding partners within the autophagy initiation complex or ULK1/PI3K complexes.
• Antibody validation: Serving as a positive control antigen in Western blot, ELISA, or immunoprecipitation experiments.
• Functional biochemical assays: Investigating lipid scramblase activity or membrane association properties of ATG9A.
• Drug screening: Supporting assay development for compounds targeting the autophagy pathway.

Researchers should optimize protein concentration, buffer conditions, and assay formats for their specific experimental systems. Refer to the product datasheet or contact Aladdin Scientific technical support for application-specific guidance.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human ATG9A Protein are essential to maintain activity and integrity. General best-practice guidelines for recombinant proteins include:

• Store at –80 °C for long-term storage upon receipt.
• Avoid repeated freeze–thaw cycles; aliquot into single-use volumes before freezing.
• Keep on ice during active use to minimize degradation.
• Centrifuge briefly before opening to collect any material from the tube walls or cap.

Note: Specific storage buffer composition, recommended working concentration, and stability data are provided in the product datasheet. Always consult the datasheet before use. If you have questions about stability under non-standard conditions, contact Aladdin Scientific technical support.

Ordering and Technical Support

Recombinant Human ATG9A Protein is available exclusively through Aladdin Scientific under SKU rp365042. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding experimental design, assay optimization, or product compatibility, the Aladdin Scientific technical support team is available to assist. Bulk quantities and custom formulations may be available upon request — please inquire directly with the sales team.

All orders are subject to Aladdin Scientific's standard terms and conditions, including shipping, returns, and payment policies. Please review the relevant policies on the Aladdin Scientific website before placing your order.

Frequently asked questions

What is the biological function of ATG9A?

ATG9A is the only multi-spanning transmembrane protein in the core autophagy machinery. It functions as a lipid scramblase and trafficking hub, cycling between the trans-Golgi network, endosomes, and autophagosome formation sites to deliver membrane lipids required for autophagosome biogenesis. It is essential for autophagic flux in mammalian cells.

What applications is Recombinant Human ATG9A Protein (rp365042) suitable for?

Recombinant Human ATG9A Protein is suitable for protein interaction studies, antibody validation (e.g., as a positive control in Western blot or ELISA), functional biochemical assays investigating lipid scramblase activity, and drug screening assays targeting the autophagy pathway. Researchers should optimize conditions for their specific assay format.

How should Recombinant Human ATG9A Protein be stored to maintain stability?

For long-term storage, keep Recombinant Human ATG9A Protein at –80 °C. Aliquot into single-use volumes before freezing to avoid repeated freeze–thaw cycles, which can reduce protein activity and integrity. Keep the protein on ice during active use. Always consult the product datasheet for buffer composition and specific stability data.

What is the difference between ATG9A and other ATG proteins?

ATG9A is unique among ATG proteins because it is the only multi-spanning transmembrane protein in the core autophagy machinery, giving it a distinct membrane-trafficking role. While most other ATG proteins are peripheral membrane or cytoplasmic proteins that transiently associate with membranes, ATG9A is an integral membrane protein that physically transports lipid bilayer components to the nascent autophagosome.

How can I place an order for rp365042 and what support is available?

Recombinant Human ATG9A Protein (rp365042) can be ordered directly through the Aladdin Scientific website or via a regional sales representative. Technical support for experimental design and assay optimization is available from the Aladdin Scientific team. Bulk quantities and custom formulations may be available on request.

References

1. Mattera R, et al. (2017). Autophagic protein ATG9A is required for mitophagy and correct mitochondrial function. Journal of Biological Chemistry.
2. Orsi A, et al. (2012). Dynamic and transient interactions of ATG9 with autophagosomes, but not Atg16L1 or its precursors. Molecular Biology of the Cell.
3. Guardia CM, et al. (2020). Structure of Human ATG9A, the Only Transmembrane Protein of the Core Autophagy Machinery. Cell Reports.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.