Recombinant Human ATOSA Protein: Properties & Research Uses

Recombinant Human ATOSA Protein is a research-grade recombinant protein derived from the human ATOSA gene, produced for use in biochemical assays, functional studies, and protein interaction experiments by research scientists worldwide.

Product Name

Recombinant Human ATOSA Protein

SKU

rp376234

Species Origin

Human (Homo sapiens)

Product Type

Recombinant Protein

Supplier Reference

absea#PP-9840

Intended Use

Research use only (RUO)

Product Overview

Recombinant Human ATOSA Protein (SKU: rp376234) is offered by Aladdin Scientific as a high-quality research reagent derived from the human ATOSA gene. Recombinant proteins such as this are produced using established expression systems to yield soluble, functional protein suitable for a wide range of laboratory applications. This product is referenced under the supplier catalogue number absea#PP-9840, providing researchers with a traceable identifier for cross-supplier sourcing and reproducibility documentation.

As a recombinant human protein, ATOSA Protein maintains the amino acid sequence of the endogenous human counterpart, making it suitable for studies that require authentic human protein biology. Researchers in cell biology, biochemistry, and molecular biology can leverage this reagent for in vitro experiments, assay development, and validation workflows.

Biochemical Properties and Production

Recombinant proteins are typically expressed in heterologous systems such as Escherichia coli, insect cells (Sf9/Hi5), or mammalian cell lines (HEK293, CHO), each conferring distinct post-translational modification profiles. The choice of expression host directly influences protein folding, glycosylation state, and biological activity. Researchers should consult the product datasheet for the specific expression system, tag information, and purity specifications for SKU rp376234 to ensure compatibility with their assay requirements.

Recombinant human proteins are generally characterised by SDS-PAGE and/or Western blot to confirm molecular weight and purity, and by activity assays where applicable. Lot-specific certificates of analysis (CoA) are provided to document purity, concentration, and quality control test results, supporting GLP-compliant experimental records.

Research Applications

Recombinant Human ATOSA Protein is suitable for a variety of research applications. Common uses for recombinant human proteins of this class include:

• Protein–protein interaction studies: Used as bait or prey in pull-down assays, co-immunoprecipitation (co-IP), or surface plasmon resonance (SPR) experiments to map interaction partners.
• Antibody validation: Serves as a positive control antigen for verifying the specificity and sensitivity of anti-ATOSA antibodies in ELISA, Western blot, and immunofluorescence assays.
• Enzyme and functional assays: Where applicable, used to characterise enzymatic activity or binding kinetics under defined in vitro conditions.
• Structural biology: Suitable as a starting material for crystallography or cryo-electron microscopy studies pending appropriate purity and buffer conditions.
• Assay development and calibration: Employed as a standard or spike-in control in quantitative assays requiring a defined amount of human protein.

Researchers should confirm suitability for each specific application by reviewing the product datasheet and, where necessary, performing pilot experiments.

Storage, Handling, and Quality Assurance

Proper storage and handling of recombinant proteins are critical for maintaining activity and preventing degradation. General best practices applicable to recombinant human proteins include:

• Store at –20 °C or –80 °C as indicated on the product datasheet; avoid repeated freeze–thaw cycles by aliquoting upon first use.
• Reconstitute lyophilised material in the recommended buffer (typically sterile PBS or the supplier-specified buffer) to the indicated concentration.
• Keep protein solutions on ice during experimental manipulation and return unused portions promptly to the recommended storage temperature.
• For long-term storage, the addition of a carrier protein (e.g., 0.1% BSA) may stabilise dilute solutions; consult the CoA for specific recommendations.

Aladdin Scientific provides lot-specific CoAs for rp376234 to ensure traceability and support compliance with institutional quality management requirements. Researchers are encouraged to record lot numbers in their laboratory notebooks for experimental reproducibility.

Ordering Information

Recombinant Human ATOSA Protein is available from Aladdin Scientific under SKU rp376234, with an internal supplier reference of absea#PP-9840. To place an order or request a quotation, contact your Aladdin Scientific account representative or visit the Aladdin Scientific online catalogue. Custom quantities, bulk pricing, and tailored formulation options may be available upon request.

For technical enquiries regarding product specifications, application suitability, or compatibility with specific assay platforms, please contact Aladdin Scientific technical support. Supporting documentation including the product datasheet and CoA can be requested at the time of purchase or downloaded from the product page.

Frequently asked questions

What is Recombinant Human ATOSA Protein used for in research?

Recombinant Human ATOSA Protein (rp376234) is used in a variety of in vitro research applications including protein–protein interaction studies, antibody validation as a positive control antigen, functional and enzymatic assays, and as a calibration standard in quantitative protein assays. Researchers should consult the product datasheet to confirm suitability for their specific application.

How should Recombinant Human ATOSA Protein be stored to maintain activity?

Recombinant Human ATOSA Protein should be stored at –20 °C or –80 °C as specified on the product datasheet. To preserve activity, avoid repeated freeze–thaw cycles by aliquoting the protein into single-use volumes upon first use. Keep protein solutions on ice during experimental handling and consult the certificate of analysis for any specific storage buffer or stabiliser recommendations.

Where can I find the datasheet and certificate of analysis for rp376234?

The product datasheet and lot-specific certificate of analysis (CoA) for Recombinant Human ATOSA Protein (rp376234) can be requested through Aladdin Scientific technical support or downloaded directly from the product page on the Aladdin Scientific website. The CoA documents purity, concentration, and quality control results for each production lot.

What expression system is used to produce Recombinant Human ATOSA Protein?

The specific expression system used for Recombinant Human ATOSA Protein (rp376234) is not detailed in the current source material. Common expression hosts for recombinant human proteins include E. coli, insect cell, and mammalian cell systems. Please refer to the product datasheet or contact Aladdin Scientific technical support for confirmed expression system details.

References

1. Aladdin Scientific product listing: Recombinant Human ATOSA Protein, SKU rp376234, supplier reference absea#PP-9840.
2. General principles of recombinant protein expression and quality control — textbook-level reference; specific citation to be confirmed by technical reviewer.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.