Recombinant Human ATP1A1-AS1 Protein: Research Guide

Recombinant Human ATP1A1-AS1 Protein is a research-grade recombinant protein derived from the human ATP1A1-AS1 locus, supplied by Aladdin Scientific (SKU: rp377451) to support molecular and cellular biology investigations.

Product Name

Recombinant Human ATP1A1-AS1 Protein

SKU

rp377451

Species Origin

Human (Homo sapiens)

Product Grade

Research grade

Supplier

Aladdin Scientific

Reference Code

absea#PP-10934

Product Overview

Recombinant Human ATP1A1-AS1 Protein (SKU: rp377451) is produced for research use and originates from the human ATP1A1-AS1 genetic locus. ATP1A1-AS1 is an antisense RNA gene located adjacent to ATP1A1, which encodes the alpha-1 subunit of the Na+/K+-ATPase pump. Antisense transcripts such as ATP1A1-AS1 have attracted increasing attention for their potential regulatory roles in gene expression modulation, particularly in the context of ion transport, cellular homeostasis, and disease-associated pathways.

This recombinant protein is manufactured to research-grade quality standards and is intended exclusively for in vitro and laboratory research applications. It is not approved for diagnostic or therapeutic use in humans or animals.

Handling, Storage, and Reconstitution

To maintain the activity and integrity of Recombinant Human ATP1A1-AS1 Protein, adhere to the following general best practices for recombinant proteins:

• Storage: Store lyophilized or liquid protein preparations at the temperature specified on the product datasheet (typically −20 °C or −80 °C for long-term storage). Avoid repeated freeze-thaw cycles, as these can cause protein aggregation and loss of activity.
• Reconstitution: Reconstitute lyophilized protein in the recommended buffer (e.g., sterile PBS or manufacturer-specified diluent) at the concentration indicated on the datasheet. Allow the vial to equilibrate to room temperature before opening. Gently swirl or pipette to dissolve; do not vortex.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycling.
• Handling: Use appropriate personal protective equipment (PPE). Consult the Safety Data Sheet (SDS) before use.

Note: Specific storage conditions, buffer composition, and concentration details are provided on the official product datasheet for rp377451. Always refer to the current datasheet for authoritative handling instructions.

Research Applications

Recombinant Human ATP1A1-AS1 Protein may be employed across a range of research applications aimed at understanding the biological functions and regulatory mechanisms of the ATP1A1-AS1 locus. Potential applications include:

• Protein–protein interaction studies: Investigating binding partners and interaction networks associated with ATP1A1-AS1-derived peptide sequences.
• Antibody development and validation: Use as an antigen standard or immunogen for generating and validating antibodies targeting ATP1A1-AS1.
• Functional assays: Probing the role of ATP1A1-AS1 in cellular pathways related to Na+/K+-ATPase regulation and ion transport.
• Western blot and ELISA controls: Serving as a positive control in immunodetection assays to confirm antibody specificity.
• Structural and biophysical studies: Characterizing secondary and tertiary structure using circular dichroism, dynamic light scattering, or other biophysical techniques.

Disclaimer: Specific validated applications for rp377451 should be confirmed with the official product datasheet or by contacting Aladdin Scientific technical support.

Quality, Purity, and Regulatory Compliance

Aladdin Scientific supplies Recombinant Human ATP1A1-AS1 Protein (rp377451) as a research-grade reagent. Research-grade recombinant proteins are produced and quality-controlled to standards appropriate for laboratory experimentation, which typically include:

• Purity assessment: SDS-PAGE or HPLC analysis to confirm purity levels as stated on the product datasheet.
• Identity confirmation: Mass spectrometry or N-terminal sequencing may be employed to verify protein identity.
• Endotoxin testing: Limulus Amebocyte Lysate (LAL) or equivalent assay to determine endotoxin content where relevant.
• Lot-specific certificates of analysis (CoA): Available upon request; contain batch-specific quality data.

This product is classified as For Research Use Only (RUO) and must not be used in clinical diagnostic procedures or therapeutic applications.

Note: Precise purity values, endotoxin limits, and quality specifications for rp377451 are detailed on the official product datasheet. Contact Aladdin Scientific customer service for the most current CoA.

Frequently asked questions

What is the recommended storage condition for Recombinant Human ATP1A1-AS1 Protein (rp377451)?

Storage conditions are specified on the product datasheet for rp377451. In general, recombinant proteins are stored at −20 °C or −80 °C to preserve stability and activity. Avoid repeated freeze-thaw cycles by preparing single-use aliquots after reconstitution. Please refer to the official datasheet or contact Aladdin Scientific technical support for exact conditions.

What research applications is Recombinant Human ATP1A1-AS1 Protein suitable for?

Recombinant Human ATP1A1-AS1 Protein (rp377451) is suitable for research applications including protein–protein interaction studies, antibody development and validation, functional assays related to Na+/K+-ATPase pathways, and use as a positive control in Western blot or ELISA experiments. Confirmed validated applications are listed on the product datasheet; contact Aladdin Scientific for clarification.

Is Recombinant Human ATP1A1-AS1 Protein approved for clinical or diagnostic use?

No. Recombinant Human ATP1A1-AS1 Protein (rp377451) is classified as For Research Use Only (RUO) and is not approved, validated, or intended for use in clinical diagnostic procedures, therapeutic applications, or any other non-research purpose.

How should I reconstitute Recombinant Human ATP1A1-AS1 Protein?

Reconstitute the lyophilized protein in the sterile buffer recommended on the product datasheet at the specified concentration. Allow the vial to reach room temperature before opening, then gently swirl or pipette to dissolve. Do not vortex. Refer to the rp377451 datasheet for precise reconstitution instructions.

Can I obtain a Certificate of Analysis (CoA) for rp377451?

Yes. Lot-specific Certificates of Analysis for Recombinant Human ATP1A1-AS1 Protein (rp377451) are available upon request from Aladdin Scientific. Please contact our customer service team with your lot number to obtain the relevant CoA documentation.

References

1. Aladdin Scientific product page: Recombinant Human ATP1A1-AS1 Protein, SKU rp377451. Reference code absea#PP-10934.
2. NCBI Gene: ATP1A1-AS1 (Homo sapiens). National Center for Biotechnology Information.
3. Kaplan JH. Biochemistry of Na,K-ATPase. Annual Review of Biochemistry. 2002;71:511-535.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.