Recombinant Human AXDND1 Protein: Key Facts & FAQs

Recombinant Human AXDND1 Protein is a research-grade recombinant protein derived from the human AXDND1 gene, associated with axonemal dynein function and available from Aladdin Scientific (SKU: rp376111) to support studies in ciliary biology, motility, and related cellular processes.

Product Name

Recombinant Human AXDND1 Protein

SKU

rp376111

Gene Name

AXDND1 (Axonemal Dynein Light Chain Domain Containing 1)

Species

Human (Homo sapiens)

Protein Family

Dynein light chain domain-containing protein family

Application

Research use only; suitable for in vitro biochemical and cell biology studies

Supplier Reference

absea#PP-9727

What Is AXDND1 and Why Is It Scientifically Relevant?

AXDND1 (Axonemal Dynein Light Chain Domain Containing 1) is a human protein encoded by the AXDND1 gene. It belongs to a family of proteins that harbor dynein light chain domains, which are structural and functional components associated with axonemal dynein motor complexes. Axonemal dyneins are large, multi-subunit ATPase motors that power the beating of cilia and flagella by driving microtubule sliding within the axoneme — the core cytoskeletal structure of these organelles.

Proper axonemal dynein function is essential for ciliary motility, and disruptions in dynein-associated proteins have been linked to primary ciliary dyskinesia (PCD) and related ciliopathies. Recombinant AXDND1 protein enables researchers to investigate the molecular role of this protein in dynein complex assembly, ciliary function, and potential disease mechanisms in a controlled in vitro setting.

Product Specifications and Storage Guidelines

Recombinant Human AXDND1 Protein (SKU: rp376111) is produced as a recombinant protein intended for research applications. As with all recombinant proteins, proper storage and handling are critical to maintaining activity and integrity.

• Intended Use: Research use only (RUO). Not for diagnostic or therapeutic purposes.
• Storage: Recombinant proteins should generally be stored at −20°C or −80°C upon receipt to preserve stability. Avoid repeated freeze-thaw cycles by aliquoting the stock upon first use.
• Handling: Thaw on ice and centrifuge briefly before opening. Reconstitute lyophilized material according to the datasheet instructions using the recommended buffer.
• Supplier Reference: absea#PP-9727

Note: Please refer to the product datasheet provided with SKU rp376111 for lot-specific concentration, purity, formulation, and recommended storage conditions, as these parameters may vary by lot.

Research Applications of Recombinant Human AXDND1 Protein

Recombinant Human AXDND1 Protein is a versatile reagent for multiple research workflows, particularly in the fields of cilia biology, cell motility, and molecular motor research. Typical in vitro research applications for recombinant dynein-associated proteins include:

• Protein–Protein Interaction Studies: Investigating binding partners and complex formation within the dynein machinery using co-immunoprecipitation, pull-down assays, or surface plasmon resonance.
• Antibody Validation: Serving as a positive control antigen for immunoassay development and antibody specificity testing.
• Structural Studies: Supporting X-ray crystallography, cryo-EM, or other structural biology experiments to elucidate domain architecture.
• Functional Assays: Exploring the contribution of AXDND1 to dynein complex assembly or ciliary beating in reconstituted systems.
• Disease Mechanism Research: Studying the molecular basis of potential links between AXDND1 variants and ciliopathies such as primary ciliary dyskinesia.

Note: Specific validated applications for this lot should be confirmed in the product datasheet. Researchers are encouraged to optimize experimental conditions for their specific assay systems.

Ordering, Quality, and Technical Support

Recombinant Human AXDND1 Protein is available exclusively from Aladdin Scientific under SKU rp376111. The following information is provided to assist with procurement and quality assurance decisions:

• Quality Standard: Aladdin Scientific recombinant proteins are manufactured and quality-controlled to meet research-grade standards. Purity and activity data are provided on the product datasheet supplied with each lot.
• How to Order: Place orders directly through the Aladdin Scientific website, by email, or via your institutional procurement system. Reference SKU rp376111 to ensure accurate fulfillment.
• Technical Documentation: A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available upon request or via the product page on the Aladdin Scientific website.
• Technical Support: For application-specific questions, reconstitution guidance, or troubleshooting, contact Aladdin Scientific's technical support team with your order number and lot number.

Frequently asked questions

What is the AXDND1 protein and what is its biological function?

AXDND1 (Axonemal Dynein Light Chain Domain Containing 1) is a human protein containing dynein light chain domains associated with axonemal dynein motor complexes. Axonemal dyneins power the beating of cilia and flagella by driving microtubule sliding within the axoneme, making AXDND1 relevant to ciliary motility and potentially to ciliopathies such as primary ciliary dyskinesia.

How should Recombinant Human AXDND1 Protein (rp376111) be stored and handled?

Recombinant Human AXDND1 Protein should be stored at −20°C or −80°C upon receipt to maintain stability. Avoid repeated freeze-thaw cycles by aliquoting the protein into single-use volumes. Thaw on ice before use, and refer to the product datasheet for lot-specific formulation and reconstitution instructions.

What research applications is Recombinant Human AXDND1 Protein suitable for?

This recombinant protein is suitable for in vitro research applications including protein–protein interaction studies, antibody validation as a positive control antigen, structural biology investigations, and functional assays exploring dynein complex assembly or ciliary function. It may also be used in disease mechanism studies related to ciliopathies. Researchers should confirm validated applications in the product datasheet and optimize conditions for their specific assay systems.

Is Recombinant Human AXDND1 Protein (rp376111) suitable for clinical or diagnostic use?

No. Recombinant Human AXDND1 Protein (rp376111) is designated for research use only (RUO) and is not approved or validated for clinical diagnostic or therapeutic applications. Any use outside of research settings must comply with applicable regulations.

How do I obtain a Certificate of Analysis or Safety Data Sheet for rp376111?

A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) for Recombinant Human AXDND1 Protein (rp376111) can be requested through the Aladdin Scientific website product page or by contacting the customer service team directly. Please have your lot number available when making the request to receive the correct documentation.

References

1. Horani A, Ferkol TW. Advances in the Genetics of Primary Ciliary Dyskinesia: Clinical Implications. Chest. 2018;154(3):645-652. doi:10.1016/j.chest.2018.05.007
2. Mitchison HM, Valente EM. Motile and non-motile cilia in human pathology: from function to phenotypes. J Pathol. 2017;241(2):294-309. doi:10.1002/path.4843
3. Kiesel P, et al. The molecular structure of mammalian primary cilia revealed by cryo-electron tomography. Nat Struct Mol Biol. 2020;27:1115-1124. doi:10.1038/s41594-020-0507-4

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.