Recombinant Human C3orf70 Protein: Research Use Guide

Recombinant Human C3orf70 Protein is a research-grade recombinant protein derived from the human chromosome 3 open reading frame 70 gene, produced for use in biochemical, cell biology, and functional studies.

Product Name

Recombinant Human C3orf70 Protein

SKU

rp373580

Source

Human C3orf70 gene (chromosome 3 open reading frame 70)

Intended Use

Research use only; not for diagnostic or therapeutic purposes

Supplier

Aladdin Scientific

Storage Recommendation

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles

Product Overview and Background

Recombinant Human C3orf70 Protein (SKU: rp373580) is produced by Aladdin Scientific as a high-quality research reagent. C3orf70, encoded by an open reading frame on human chromosome 3, represents a protein whose precise biological functions are still under active investigation. As with many chromosome-mapped open reading frame proteins, C3orf70 may play roles in cellular regulatory or signaling pathways, making it of interest to researchers exploring novel gene functions and protein interactions.

This recombinant form is designed to provide researchers with a reliable, consistently produced protein source suitable for a range of in vitro biochemical and cell-based assays. The product is intended strictly for research use and is not approved for diagnostic or therapeutic applications.

Protein Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human C3orf70 Protein is manufactured under controlled conditions to ensure lot-to-lot consistency and high purity. Key quality attributes to be confirmed in the product datasheet include:

• Purity: Typically assessed by SDS-PAGE and/or HPLC; researchers should consult the current Certificate of Analysis (CoA) for the specific lot purity value.
• Expression System: The expression host (e.g., E. coli, baculovirus, or mammalian system) determines post-translational modification status; refer to the product datasheet for confirmed expression system details.
• Formulation: Supplied in a defined buffer system; the exact buffer composition and any stabilizing additives are detailed on the product datasheet.
• Molecular Weight: The predicted molecular weight based on the amino acid sequence should be verified by the CoA; apparent molecular weight on SDS-PAGE may differ due to modifications or tags.

Researchers are strongly advised to review the Certificate of Analysis provided with each shipment to verify purity, concentration, and endotoxin levels before use.

Handling, Reconstitution, and Storage Protocol

Proper handling of Recombinant Human C3orf70 Protein is essential to preserve biological activity and ensure reproducible experimental results. Follow these general best-practice guidelines:

1. Receiving: Upon receipt, immediately inspect the shipment for integrity. If supplied lyophilized, confirm the vial is intact and the material appears as expected.
2. Reconstitution: If the product is lyophilized, reconstitute with the recommended volume of sterile, ultrapure water or the buffer specified on the datasheet. Allow the vial to equilibrate to room temperature before opening. Gently swirl or pipette to dissolve; do not vortex vigorously as this may denature the protein.
3. Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles. Label aliquots clearly with date, concentration, and lot number.
4. Storage: Store working aliquots at -20°C for short-term use or at -80°C for long-term storage. Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.
5. Handling on Ice: Keep protein on ice during experimental setup to minimize degradation.

Always work under aseptic conditions when handling recombinant proteins intended for cell-based assays to prevent microbial contamination.

Recommended Applications

Recombinant Human C3orf70 Protein is suitable for a range of research applications, subject to validation in each laboratory's specific experimental system. Potential applications include:

• Western Blotting (WB): Can be used as a positive control to validate antibody specificity against C3orf70.
• ELISA: Suitable as a standard or calibrator in sandwich or direct ELISA formats for quantifying C3orf70 levels.
• Protein–Protein Interaction Studies: Can be employed in pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance (SPR) experiments to identify binding partners.
• Functional Assays: Where biological activity has been confirmed, the protein may be used in cell-based or cell-free functional assays to study the role of C3orf70 in cellular pathways.
• Antibody Production and Validation: Suitable as an immunogen or standard for generating and validating research-grade antibodies.

Users are responsible for validating the suitability of this product for their specific application. Application-specific validation data, if available, are provided in the product datasheet.

Troubleshooting and Quality Assurance

If unexpected results are observed when working with Recombinant Human C3orf70 Protein, consider the following troubleshooting steps:

• Low or No Signal in WB/ELISA: Verify antibody compatibility and confirm the protein has not undergone degradation. Check storage conditions and the number of freeze-thaw cycles the aliquot has experienced.
• Protein Aggregation: If visible aggregates are observed, the protein may have been stored improperly or subjected to temperature fluctuations. Centrifuge briefly at low speed (e.g., 5,000 × g) to pellet aggregates and use only the supernatant.
• Unexpected Band Size on SDS-PAGE: Apparent molecular weight on SDS-PAGE may differ from the predicted size due to fusion tags, glycosylation, or other post-translational modifications. Refer to the CoA for the confirmed band size.
• Endotoxin Concerns: For cell-based assays, high endotoxin levels can confound results. Confirm endotoxin levels from the CoA and use an endotoxin-removal kit if necessary.

For unresolved issues, contact Aladdin Scientific technical support with the product lot number and a description of the experimental protocol. Aladdin Scientific is committed to providing timely technical assistance to ensure research success.

Frequently asked questions

What is C3orf70 and why is it relevant to research?

C3orf70 (chromosome 3 open reading frame 70) is a human protein-coding gene whose product's precise biological function is currently under investigation. Researchers study it to understand its potential roles in cellular signaling, regulation, or disease-associated pathways. Recombinant protein tools enable functional and interaction studies.

How should I store Recombinant Human C3orf70 Protein to maintain activity?

Store the protein at -80°C for long-term preservation, or at -20°C for short-term use. Prepare small, single-use aliquots upon first thaw to avoid repeated freeze-thaw cycles, which can cause aggregation and loss of biological activity. Keep the protein on ice during experiments.

Which applications is Recombinant Human C3orf70 Protein validated for?

The protein is potentially suitable for Western blotting, ELISA, protein–protein interaction studies, and antibody production. However, users should verify suitability for their specific application and experimental system. Refer to the product datasheet and Certificate of Analysis for any application-specific validation data provided by Aladdin Scientific.

Can I use this protein in cell-based assays?

Yes, the protein may be used in cell-based assays where appropriate, but you should first confirm the endotoxin level from the Certificate of Analysis. High endotoxin levels can trigger innate immune responses in cell culture and confound experimental results. If endotoxin levels are a concern, treat the protein with an endotoxin-removal resin before use.

How do I place an order for rp373580 through Aladdin Scientific?

You can order Recombinant Human C3orf70 Protein (SKU: rp373580) directly through the Aladdin Scientific website or by contacting your regional Aladdin Scientific sales representative. For bulk orders or custom requirements, please reach out to the Aladdin Scientific sales or technical support team for pricing and availability.

References

1. Aladdin Scientific Product Datasheet: Recombinant Human C3orf70 Protein, SKU rp373580. Aladdin Scientific.
2. UniProt Consortium. UniProtKB entry for human C3orf70 protein. UniProt.
3. NCBI Gene. C3orf70 chromosome 3 open reading frame 70 [Homo sapiens]. National Center for Biotechnology Information.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.