Recombinant Human CEP192 Protein: Research Guide & Applications

Recombinant Human CEP192 Protein is a key centrosomal scaffold protein that orchestrates centriole duplication, pericentriolar material assembly, and mitotic spindle organization, making it an essential research tool for investigators studying centrosome biology and cell division.

Protein Name

Centrosomal Protein 192 (CEP192)

Species

Human (Homo sapiens)

Protein Form

Recombinant protein

Primary Function

Centrosome maturation, centriole duplication, and spindle assembly regulation

Key Interacting Partners

Aurora A kinase, PLK1, AURKA, pericentrin

Recommended Storage

Store at -80°C; avoid repeated freeze-thaw cycles (general guidance — verify with datasheet)

SKU

rp367036

Biological Background and Function of CEP192

CEP192 (Centrosomal Protein 192) is a large scaffolding protein that localizes to the pericentriolar material (PCM) of the centrosome throughout the cell cycle. It plays an indispensable role in centrosome maturation — the process by which the PCM expands dramatically at the onset of mitosis to support robust bipolar spindle assembly.

CEP192 acts as a critical platform for the recruitment of Aurora A kinase (AURKA) and Polo-like kinase 1 (PLK1) to the centrosome. These kinase interactions are essential for phosphorylation cascades that drive PCM scaffold assembly and centriole duplication licensing. Loss of CEP192 function has been shown to cause mitotic spindle defects, chromosome missegregation, and aberrant centriole numbers, implicating CEP192 in genomic stability maintenance.

In addition to its mitotic roles, CEP192 participates in the formation of primary cilia by contributing to basal body maturation, positioning it as a protein of interest in studies of ciliopathies and developmental signaling pathways such as Hedgehog signaling.

Biochemical Properties and Product Specifications

Recombinant Human CEP192 Protein (SKU: rp367036) is produced as a recombinant form of the human CEP192 gene product. Researchers should consult the official product datasheet for confirmed details on expression system, tag configuration, purity grade, molecular weight, and formulation buffer, as these parameters are critical for experimental design.

General considerations for recombinant CEP192 include its large molecular weight (the native human protein is encoded by a gene spanning a large open reading frame), which may influence gel migration behavior and antibody detection. Purity is typically assessed by SDS-PAGE and/or HPLC, and activity may be evaluated through binding assays with known interacting partners such as AURKA.

Specific lot-dependent values for purity percentage, endotoxin level, and concentration should be obtained from the Certificate of Analysis (CoA) accompanying each shipment. Always verify the exact specifications with Aladdin Scientific prior to experimental use.

Recommended Handling, Storage, and Reconstitution Protocol

Proper handling of Recombinant Human CEP192 Protein is essential to preserve its structural integrity and functional activity. The following guidelines represent general best practices for recombinant proteins of this class; always defer to the product-specific datasheet for authoritative instructions.

• Storage: Upon receipt, aliquot immediately to minimize freeze-thaw cycles and store at -80°C for long-term stability. Short-term storage (up to 1 week) may be possible at -20°C or 4°C depending on formulation — verify with the datasheet.
• Reconstitution: If supplied lyophilized, reconstitute with the volume of sterile water or buffer specified on the datasheet. Allow the vial to equilibrate to room temperature before opening, and mix gently by pipetting; avoid vortexing.
• Working Aliquots: Prepare single-use aliquots prior to first use to avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• Carrier Protein: For low-concentration working solutions, consider supplementing with 0.1% BSA to prevent surface adsorption and loss of protein.
• Contamination Control: Use sterile, low-binding tubes and pipette tips to minimize non-specific protein adsorption.

If protein aggregation is suspected, centrifuge briefly at 10,000 × g at 4°C prior to use and recover the soluble supernatant fraction.

Research Applications

Recombinant Human CEP192 Protein is a versatile tool applicable across multiple research contexts in cell biology and biochemistry:

• Protein–Protein Interaction Studies: CEP192 is an ideal bait protein for pull-down assays, co-immunoprecipitation validation, and surface plasmon resonance (SPR) or bio-layer interferometry (BLI) experiments aimed at characterizing its interactions with Aurora A, PLK1, pericentrin, and other PCM components.
• Biochemical Reconstitution Assays: Recombinant CEP192 can be incorporated into in vitro reconstitution systems to study centrosome assembly, kinase recruitment, and PCM scaffold dynamics.
• Antibody Validation: Recombinant protein serves as a positive control antigen for validating CEP192-specific antibodies used in Western blotting, immunofluorescence, and immunoprecipitation applications.
• Structural Biology: The purified protein may serve as a substrate for cryo-EM, X-ray crystallography (domain fragments), or hydrogen-deuterium exchange mass spectrometry (HDX-MS) studies of CEP192 domain architecture.
• Drug Discovery Screens: CEP192's role in Aurora A recruitment makes it relevant for screening small-molecule inhibitors targeting the centrosome maturation pathway, a strategy of interest in oncology research.

Frequently asked questions

What is the biological function of CEP192 in human cells?

CEP192 is a centrosomal scaffold protein that is essential for centrosome maturation, centriole duplication, and bipolar mitotic spindle assembly. It recruits key mitotic kinases, including Aurora A and PLK1, to the pericentriolar material, and also contributes to primary cilia formation via basal body maturation.

What research applications is Recombinant Human CEP192 Protein suitable for?

Recombinant Human CEP192 Protein is suitable for protein–protein interaction studies (pull-down, co-IP, SPR/BLI), in vitro biochemical reconstitution of centrosome assembly, antibody validation (Western blot, immunofluorescence), structural biology investigations, and small-molecule drug discovery screens targeting the centrosome maturation pathway.

How should Recombinant Human CEP192 Protein be stored to maintain activity?

As a general guideline for recombinant proteins, Recombinant Human CEP192 Protein should be stored at -80°C for long-term preservation and aliquoted upon receipt to avoid repeated freeze-thaw cycles. Always refer to the product datasheet and Certificate of Analysis for storage conditions specific to each lot.

Why is CEP192 relevant to cancer biology and drug discovery?

Centrosome amplification and abnormal mitotic spindle formation are hallmarks of many cancer types. Because CEP192 orchestrates Aurora A kinase recruitment and PCM expansion during mitosis, it represents a potential target for therapeutic strategies aimed at disrupting centrosome maturation in cancer cells. Recombinant CEP192 protein supports such drug discovery efforts by enabling in vitro binding and inhibition assays.

Where can I find the confirmed purity, molecular weight, and formulation details for SKU rp367036?

Confirmed lot-specific specifications — including purity, molecular weight, endotoxin level, and buffer formulation — are provided on the product datasheet and the Certificate of Analysis (CoA) available through Aladdin Scientific. Contact Aladdin Scientific customer service or visit the product page for SKU rp367036 to request these documents.

References

1. Gomez-Ferreria MA, et al. Human CEP192 interplays with GTubolin- and CDK5RAP2-associated proteins during centriole duplication. J Cell Sci. 2012.
2. Joukov V, et al. The CEP192-organized aurora A-Plk1 cascade is essential for centrosome cycle and bipolar spindle assembly. Mol Cell. 2014.
3. Zhu F, et al. The mammalian SPD-2 ortholog Cep192 regulates centrosome biogenesis. Curr Biol. 2008.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.