Recombinant Human CEP20 Protein: Properties & Research Uses

Recombinant Human CEP20 Protein is a recombinantly produced form of the human centrosomal protein 20, supporting research into centrosome function, ciliogenesis, and cell cycle regulation.

Protein Name

Recombinant Human CEP20 Protein

Species

Human (Homo sapiens)

Production Method

Recombinant expression

Primary Research Area

Centrosome biology, ciliogenesis, cell cycle

Catalog SKU

rp366162

Supplier Reference

absea#PP-1782

Biological Background of CEP20

CEP20, also known as Centrosomal Protein 20, is a protein encoded by the CEP20 gene in humans. As its name implies, CEP20 localizes to the centrosome, the primary microtubule-organizing center (MTOC) of animal cells. The centrosome plays essential roles in mitotic spindle assembly, chromosome segregation, and the formation of primary cilia.

Primary cilia are antenna-like organelles that project from the surface of most quiescent mammalian cells and serve as key hubs for signaling pathways including Hedgehog, Wnt, and PDGF receptor signaling. CEP20 has been implicated in the regulation of ciliogenesis—the biogenesis and maintenance of primary cilia—making it a protein of significant interest in studies of ciliopathies, developmental biology, and cancer cell biology.

Dysregulation of centrosomal proteins, including CEP20, is associated with defects in cell division, abnormal cilia formation, and has been linked to a range of human diseases. Understanding the precise molecular functions of CEP20 requires high-quality recombinant protein tools suitable for biochemical and cell-based assays.

Biochemical Properties and Production

Recombinant Human CEP20 Protein (SKU: rp366162) is produced using recombinant expression systems that enable the generation of well-defined, reproducible protein material. Recombinant production ensures consistency between lots, which is critical for quantitative biochemical assays and comparative studies across experimental replicates.

The protein corresponds to the human CEP20 sequence (Homo sapiens) and is intended for use in research settings. Researchers should consult the product datasheet for specific details regarding the expression host, tag configuration, molecular weight, purity grade, formulation buffer, and recommended storage conditions, as these parameters are critical for experimental design and reagent compatibility.

Standard quality control assessments for recombinant proteins of this class typically include SDS-PAGE analysis to confirm molecular weight and purity, and may include functional activity assays where applicable. Endotoxin levels are generally assessed to ensure suitability for cell-based applications.

Research Applications

Recombinant Human CEP20 Protein is a versatile reagent suited to multiple research applications in cell biology and biochemistry:

• Protein–protein interaction studies: CEP20 can be used as bait or prey in pull-down assays and co-immunoprecipitation experiments to identify binding partners within the centrosomal proteome.
• Antibody validation: Recombinant CEP20 protein serves as a positive control antigen for validating anti-CEP20 antibodies used in western blotting, immunofluorescence, and ELISA.
• Structural and biophysical studies: Purified recombinant protein can be used in biophysical assays such as surface plasmon resonance (SPR) or isothermal titration calorimetry (ITC) to characterize interaction kinetics and thermodynamics.
• Functional assays: Investigators studying centrosome duplication, spindle pole integrity, or ciliogenesis may use recombinant CEP20 in cell-free reconstitution experiments.
• Ciliopathy and disease research: As a component of centrosome-cilia axis biology, CEP20 protein is relevant to research into ciliopathies such as Bardet-Biedl syndrome and Joubert syndrome, where centrosomal protein dysfunction is a known pathogenic mechanism.

Handling, Storage, and Quality Considerations

To maintain the integrity and activity of Recombinant Human CEP20 Protein, researchers should adhere to the handling and storage recommendations provided in the official product datasheet. General best practices for recombinant proteins include:

• Storing the protein at the recommended temperature (typically −20 °C or −80 °C) to prevent degradation.
• Avoiding repeated freeze–thaw cycles by preparing single-use aliquots upon first thaw.
• Keeping the protein on ice during experimental procedures to minimize activity loss.
• Centrifuging briefly before use if precipitation is suspected.

Purity, endotoxin content, and biological activity data are available on the product certificate of analysis (CoA). Researchers performing cell-based assays should pay particular attention to endotoxin levels to avoid confounding inflammatory responses. For any technical questions regarding this product, Aladdin Scientific's technical support team is available to assist.

Frequently asked questions

What is CEP20 and why is it important in cell biology research?

CEP20 (Centrosomal Protein 20) is a centrosome-associated protein involved in organizing microtubules and regulating ciliogenesis. It is important because defects in centrosomal proteins like CEP20 are linked to ciliopathies, cell division errors, and developmental diseases, making it a relevant target for mechanistic and translational research.

What applications is Recombinant Human CEP20 Protein suitable for?

Recombinant Human CEP20 Protein is suitable for protein–protein interaction studies (pull-down, co-IP), antibody validation (western blot, ELISA, immunofluorescence), biophysical assays (SPR, ITC), and cell-free functional reconstitution experiments. It is also used in disease-focused research on ciliopathies and centrosome dysfunction.

How should Recombinant Human CEP20 Protein be stored to maintain stability?

The protein should be stored according to the conditions specified on the product datasheet, generally at −20 °C or −80 °C. Repeated freeze–thaw cycles should be avoided by aliquoting the protein upon first thaw. Always keep the protein on ice during bench work to minimize degradation.

Is Recombinant Human CEP20 Protein suitable for use in cell-based assays?

Recombinant Human CEP20 Protein may be suitable for cell-based assays; however, researchers should verify the endotoxin content specified in the certificate of analysis before use. Low endotoxin levels are essential to avoid non-specific inflammatory responses that could confound experimental results. Please consult the product datasheet or contact Aladdin Scientific technical support for guidance.

References

1. Andersen JS, et al. Proteomic characterization of the human centrosome by protein correlation profiling. Nature. 2003;426(6966):570–574. doi:10.1038/nature02166
2. Nigg EA, Raff JW. Centrioles, centrosomes, and cilia in health and disease. Cell. 2009;139(4):663–678. doi:10.1016/j.cell.2009.10.036
3. Reiter JF, Leroux MR. Genes and molecular pathways underpinning ciliopathies. Nature Reviews Molecular Cell Biology. 2017;18(9):533–547. doi:10.1038/nrm.2017.60

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.