Recombinant Human CHMP5 Protein: Research Guide & Applications

Recombinant Human CHMP5 Protein is a key component of the ESCRT-III complex involved in multivesicular body biogenesis, membrane remodeling, and endosomal cargo sorting, making it an essential research tool for studying lysosomal degradation pathways and related cellular processes.

Product Name

Recombinant Human CHMP5 Protein

SKU

rp365939

Protein Family

ESCRT-III / Charged Multivesicular Body Protein (CHMP) family

Biological Function

Involved in multivesicular body (MVB) biogenesis and endosomal sorting via the ESCRT-III complex

Research Relevance

Critical for studies on lysosomal degradation, autophagy, viral budding, and cytokinesis

Species

Human (Homo sapiens)

Format

Recombinant protein; refer to product datasheet for detailed formulation

Biological Background of CHMP5

CHMP5 (Charged Multivesicular Body Protein 5), also known as SNF7 domain-containing protein, is a member of the ESCRT-III (Endosomal Sorting Complexes Required for Transport III) family. ESCRT-III proteins are highly conserved across eukaryotes and are essential for membrane deformation and scission events that occur away from the cytoplasm.

CHMP5 plays a modulatory role within the ESCRT-III complex, working in concert with other CHMP family members (such as CHMP1, CHMP2, CHMP3, and CHMP4) to facilitate the sorting of ubiquitinated transmembrane cargo proteins into intraluminal vesicles (ILVs) of multivesicular bodies (MVBs). These MVBs subsequently fuse with lysosomes to enable degradation of the sorted cargo, a process critical for receptor downregulation and signal termination.

Beyond MVB biogenesis, CHMP5 has been implicated in additional cellular processes including autophagy, cytokinesis, nuclear envelope reformation, and the budding of certain enveloped viruses such as HIV-1. Its broad involvement in membrane remodeling events underscores its significance as a research target in cell biology and virology.

Protein Properties and Quality Considerations

Recombinant Human CHMP5 Protein (SKU: rp365939) is produced using recombinant expression systems to ensure consistency and reproducibility across experiments. Researchers should consult the product-specific datasheet for confirmed details on expression host, tag configuration, purity grade, molecular weight, and formulation buffer, as these parameters are critical for experimental design.

When evaluating recombinant CHMP5 for use, consider the following general quality attributes:

• Purity: High-purity preparations minimize background interference in binding assays, pull-down experiments, and structural studies.
• Biological Activity: Functional validation (e.g., ESCRT-III complex reconstitution assays or liposome binding assays) confirms that the protein retains native-like activity.
• Endotoxin Level: Low endotoxin content is especially important for cell-based assays to avoid confounding inflammatory responses.
• Storage and Stability: Recombinant proteins are typically stored at -80°C in aliquots to avoid freeze-thaw degradation. Always refer to the specific datasheet for storage recommendations.

For the most accurate and up-to-date specifications of rp365939, refer to the Aladdin Scientific product datasheet or contact technical support.

Research Applications

Recombinant Human CHMP5 Protein is a versatile reagent suitable for a range of in vitro and biochemical research applications. Key application areas include:

• ESCRT-III Complex Reconstitution: CHMP5 can be used to reconstitute components of the ESCRT-III machinery in vitro, enabling mechanistic studies of membrane deformation and scission.
• Protein–Protein Interaction Studies: Co-immunoprecipitation, yeast two-hybrid validation, and surface plasmon resonance (SPR) assays can leverage recombinant CHMP5 to map interactions with binding partners such as VPS4, LIP5/CHMP5-interacting proteins, and other ESCRT subunits.
• Structural Biology: Purified recombinant CHMP5 can serve as material for X-ray crystallography, cryo-electron microscopy (cryo-EM), or NMR studies aimed at resolving ESCRT-III assembly mechanisms.
• Biochemical Assays: CHMP5 can be employed as an antigen for antibody validation, as a positive control in Western blotting, or as a standard in ELISA development.
• Drug Discovery: Screening for small molecules that disrupt CHMP5–ESCRT interactions may yield candidates relevant to antiviral therapy or cancer biology, given ESCRT involvement in tumor suppressor receptor degradation.

Handling, Storage, and Reconstitution Guidelines

Proper handling of Recombinant Human CHMP5 Protein is essential to preserve its integrity and biological activity. The following general best-practice guidelines apply; always cross-reference with the product-specific datasheet (rp365939) for definitive instructions:

1. Receipt and Inspection: Upon receipt, inspect the vial for integrity. Record the lot number and verify that the product has been shipped under appropriate cold-chain conditions as specified.
2. Reconstitution: If supplied in lyophilized form, centrifuge the vial briefly before opening to collect all material. Reconstitute with the buffer recommended in the datasheet (typically sterile PBS or a low-salt buffer with a carrier protein such as BSA). Allow the protein to dissolve gently; avoid vortexing.
3. Aliquoting: Divide the reconstituted protein into single-use aliquots immediately after reconstitution to minimize repeated freeze-thaw cycles, which can cause aggregation and loss of activity.
4. Storage: Store aliquots at -80°C for long-term preservation. For short-term use (up to one week), storage at 4°C may be acceptable; consult the datasheet for confirmed stability data.
5. Thawing: Thaw aliquots on ice or at 4°C. Do not thaw at 37°C or by repeated freeze-thaw cycling.
6. Working Concentration: Titrate the protein concentration empirically for each assay type, as optimal concentrations vary by application.

Regulatory and Safety Information

Recombinant Human CHMP5 Protein (rp365939) is intended for Research Use Only (RUO) and is not approved for use in diagnostic, therapeutic, or clinical procedures. Users must comply with all applicable institutional biosafety guidelines and local regulations when handling recombinant proteins.

As a recombinant protein of human origin, standard laboratory safety precautions apply:

• Handle with appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection.
• Dispose of all biological materials in accordance with institutional and governmental biohazard waste disposal regulations.
• Consult the Safety Data Sheet (SDS) provided by Aladdin Scientific for detailed hazard and handling information specific to this product.

For procurement, pricing, and availability inquiries, contact Aladdin Scientific directly or visit the product page for rp365939 on the Aladdin Scientific website.

Frequently asked questions

What is the biological function of CHMP5 in human cells?

CHMP5 is a member of the ESCRT-III complex and plays a regulatory role in multivesicular body (MVB) biogenesis by facilitating the sorting of ubiquitinated membrane proteins into intraluminal vesicles for lysosomal degradation. It is also involved in cytokinesis, nuclear envelope reformation, autophagy, and the budding of certain enveloped viruses such as HIV-1.

What research applications is Recombinant Human CHMP5 Protein (rp365939) suitable for?

Recombinant Human CHMP5 Protein is suitable for in vitro ESCRT-III complex reconstitution studies, protein–protein interaction mapping (e.g., co-IP, SPR), structural biology investigations (X-ray crystallography, cryo-EM), antibody validation, Western blot positive controls, ELISA development, and drug discovery screening programs targeting ESCRT-related pathways.

How should Recombinant Human CHMP5 Protein be stored to maintain activity?

For long-term storage, aliquots of Recombinant Human CHMP5 Protein should be kept at -80°C. Repeated freeze-thaw cycles should be avoided as they can cause protein aggregation and loss of biological activity. For short-term use, storage at 4°C may be acceptable; always refer to the product-specific datasheet for rp365939 for confirmed storage conditions and stability data.

Is Recombinant Human CHMP5 Protein approved for clinical or diagnostic use?

No. Recombinant Human CHMP5 Protein (rp365939) from Aladdin Scientific is designated for Research Use Only (RUO) and is not intended or approved for diagnostic, therapeutic, or clinical applications. Users must adhere to all applicable biosafety and regulatory requirements when handling this product.

Where can I find detailed specifications such as purity, molecular weight, and formulation for rp365939?

Detailed specifications including expression host, purity grade, molecular weight, tag information, formulation buffer, and activity data for rp365939 are provided in the product-specific datasheet available on the Aladdin Scientific website. For additional technical assistance, please contact Aladdin Scientific's technical support team directly.

References

1. Hurley JH. ESCRTs are everywhere. EMBO J. 2015;34(19):2398-2407. doi:10.15252/embj.201592484
2. Henne WM, Stenmark H, Emr SD. Molecular mechanisms of the membrane sculpting ESCRT pathway. Cold Spring Harb Perspect Biol. 2013;5(9):a016766. doi:10.1101/cshperspect.a016766
3. Shim S, Kimpler LA, Bhatt V, et al. CHMP5 is essential for late endosome function and Down-regulation of receptor signaling during spermatogenesis. J Cell Biol. 2006;172(7):1045-1059. doi:10.1083/jcb.200508110
4. Aladdin Scientific. Recombinant Human CHMP5 Protein, SKU rp365939. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.