Recombinant Human GAP43 Protein: FAQs & Key Facts

Recombinant Human GAP43 Protein is a research-grade recombinant protein representing the human growth-associated protein 43, a key neuronal membrane phosphoprotein widely studied for its roles in axonal growth, synaptic plasticity, and neural regeneration.

Product Name

Recombinant Human GAP43 Protein

SKU

rp367912

Protein Target

GAP43 (Growth Associated Protein 43), also known as Neuromodulin or B-50

Species Origin

Human (Homo sapiens)

Application

Suitable for use as a standard or immunogen in neuroscience research applications

Storage Recommendation

Avoid repeated freeze-thaw cycles; store as directed on product datasheet

What Is GAP43 and Why Is It Important in Research?

GAP43 (Growth Associated Protein 43), also commonly referred to as Neuromodulin or B-50, is a neuron-specific phosphoprotein predominantly expressed in growth cones of developing and regenerating axons. It plays a central role in signal transduction at the synaptic membrane, axonal pathfinding, and synaptic plasticity.

GAP43 expression is markedly upregulated during periods of axonal growth and nerve regeneration, making it a widely used marker for neuronal development and regenerative capacity in both in vitro and in vivo experimental models. Researchers studying neurodegenerative diseases, traumatic nerve injury, and synaptic remodeling frequently include GAP43 as a key readout or experimental tool.

Recombinant Human GAP43 Protein (rp367912) enables standardized, reproducible experiments by providing a well-defined human-sequence protein suitable for use as a positive control, immunogen, or biochemical standard.

Product Specifications and Quality Information

Recombinant Human GAP43 Protein (rp367912) is produced using recombinant expression technology to deliver a research-grade protein of human origin. Key specification details are provided in the product datasheet accompanying each lot.

Researchers should consult the lot-specific Certificate of Analysis (CoA) for confirmed purity, concentration, formulation buffer, and endotoxin levels prior to use. Purity is typically assessed by SDS-PAGE. As formulations and lot details can vary, always reference the most current datasheet available on the Aladdin Scientific product page for rp367912.

For downstream applications requiring high purity or specific buffer conditions—such as structural studies or in vivo work—users are advised to contact Aladdin Scientific technical support to confirm suitability before ordering.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling of Recombinant Human GAP43 Protein are essential to maintain activity and integrity throughout its shelf life. General best practices for recombinant proteins apply:

• Storage temperature: Store the protein at the temperature specified on the product datasheet (typically −20°C or −80°C for long-term storage).
• Freeze-thaw cycles: Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity. Aliquot the protein upon first thaw for single-use portions.
• Reconstitution: If supplied lyophilized, reconstitute using the buffer and volume recommended in the datasheet. Allow the vial to equilibrate to room temperature before opening, and mix gently rather than vortexing.
• Working aliquots: Prepare small single-use aliquots and store at −80°C to preserve protein stability.

Always consult the product-specific datasheet for the definitive storage and reconstitution instructions for each lot of rp367912.

Common Research Applications for GAP43 Recombinant Protein

Recombinant Human GAP43 Protein supports a range of research applications in neuroscience and related fields. Typical uses include:

• Positive control: Used as a reference standard in Western blot assays to confirm antibody specificity and validate assay performance when studying endogenous GAP43.
• Immunogen: Can serve as an antigen for generating or validating anti-GAP43 antibodies in immunization protocols.
• ELISA standard: Functions as a quantitation standard in enzyme-linked immunosorbent assays designed to measure GAP43 levels in biological samples.
• Biochemical studies: Supports in vitro studies of GAP43 protein-protein interactions, calmodulin binding, membrane association, and phosphorylation events.
• Assay development: Useful for developing and optimizing detection assays in drug discovery pipelines targeting neuroregeneration or synaptic plasticity pathways.

Users are encouraged to verify application-specific suitability by reviewing the datasheet and, where necessary, contacting Aladdin Scientific technical support.

Ordering, Availability, and Technical Support

Recombinant Human GAP43 Protein is available from Aladdin Scientific under SKU rp367912. To place an order, visit the Aladdin Scientific website and search by SKU or product name, or contact your regional Aladdin Scientific representative.

Bulk or custom orders: For large-scale or custom requirements (e.g., specific buffer formulations, higher concentration, or endotoxin-free grades), please contact Aladdin Scientific customer service to discuss feasibility and pricing.

Technical support: For questions regarding experimental design, application suitability, or troubleshooting, Aladdin Scientific's technical support team is available to assist. Include your lot number and CoA details when contacting support for fastest resolution.

Returns and quality issues: If you receive a product that does not meet the stated specifications, please refer to Aladdin Scientific's return and refund policy and contact customer service with your order details and a description of the issue.

Frequently asked questions

What is Recombinant Human GAP43 Protein and what does GAP43 do?

Recombinant Human GAP43 Protein (rp367912) is a recombinant form of the human growth-associated protein 43, a neuron-specific membrane phosphoprotein critical for axonal growth cone function, synaptic plasticity, and nerve regeneration. It is widely used as a neuronal developmental and regeneration marker in research.

What are the recommended applications for Recombinant Human GAP43 Protein?

This protein is suitable for use as a positive control in Western blot assays, as an immunogen for antibody generation or validation, as an ELISA quantitation standard, and for in vitro biochemical studies of GAP43 interactions and phosphorylation. Please verify specific application suitability by consulting the product datasheet or contacting technical support.

How should Recombinant Human GAP43 Protein be stored to maintain stability?

Store the protein according to the conditions specified on the product datasheet, typically at −20°C or −80°C for long-term storage. Avoid repeated freeze-thaw cycles by preparing single-use aliquots upon first thaw. If the product is supplied lyophilized, reconstitute using the recommended buffer as described in the datasheet.

What is the SKU for Recombinant Human GAP43 Protein at Aladdin Scientific?

Recombinant Human GAP43 Protein is available under SKU rp367912 at Aladdin Scientific. You can search this SKU directly on the Aladdin Scientific website to access the full product page, datasheet, and ordering information.

Can I request bulk quantities or custom formulations of Recombinant Human GAP43 Protein?

Yes. For bulk quantities, custom buffer formulations, specific concentration requirements, or endotoxin-free preparations, please contact Aladdin Scientific customer service directly to discuss your requirements, feasibility, and pricing.

How do I troubleshoot if I experience unexpected results with Recombinant Human GAP43 Protein?

First, verify that storage and handling conditions were followed as specified in the datasheet, and confirm that the lot-specific CoA specifications meet your application requirements. If issues persist, contact Aladdin Scientific technical support with your lot number, CoA, experimental conditions, and a description of the results observed for personalized troubleshooting assistance.

References

1. Skene JH. Axonal growth-associated proteins. Annual Review of Neuroscience. 1989;12:127-156. (General reference on GAP43 biology; verify applicability to this specific recombinant product.)
2. Benowitz LI, Routtenberg A. GAP-43: an intrinsic determinant of neuronal development and plasticity. Trends in Neurosciences. 1997;20(2):84-91. (Classic review on GAP43 function; specific data not sourced from product material.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.