Recombinant Human GIMAP5 Protein: Function & Research Use

Recombinant Human GIMAP5 Protein is a biologically relevant research reagent that plays a critical role in immune cell survival, particularly in the maintenance and homeostasis of T lymphocytes.

Protein Name

GTPase, IMAP Family Member 5 (GIMAP5)

Product SKU

rp371132

Species

Human (Homo sapiens)

Protein Family

GIMAP (GTPase of the Immunity-Associated Protein) family

Primary Biological Role

Regulation of immune cell survival and apoptosis, particularly in T lymphocytes

Research Applications

Immunology, T cell biology, apoptosis, lymphocyte homeostasis

What Is GIMAP5? Biological Background

GIMAP5, or GTPase of the Immunity-Associated Protein 5, is a member of the GIMAP protein family — a group of small GTPases expressed predominantly in immune cells, particularly lymphocytes. The GIMAP family is characterized by a conserved AIG1 (androgen-induced gene 1) domain and GTP-binding motifs, placing these proteins within a functionally distinct branch of the GTPase superfamily.

In humans, GIMAP5 is expressed mainly in peripheral blood lymphocytes and plays an essential role in regulating T cell survival and homeostasis. Studies in animal models have highlighted the critical importance of GIMAP5: loss-of-function mutations are associated with lymphopenia, heightened apoptosis of T cells, and susceptibility to autoimmune-like conditions. GIMAP5 is thought to act as a pro-survival factor, influencing mitochondrial integrity and protecting lymphocytes from programmed cell death.

Given its central role in immune regulation, GIMAP5 represents a valuable target for research into T cell development, immune deficiency, autoimmunity, and the molecular mechanisms underlying lymphocyte survival.

Product Description and Specifications

Aladdin Scientific's Recombinant Human GIMAP5 Protein (SKU: rp371132) is produced using recombinant expression technology to deliver a high-quality, research-grade protein suitable for a wide range of in vitro studies. The product is designed to support immunology research, protein interaction studies, and functional assays requiring active human GIMAP5.

Key product attributes include:

• Species: Human (Homo sapiens)
• Protein: GIMAP5 (GTPase, IMAP Family Member 5)
• Form: Recombinant protein
• Application: Suitable for use in biochemical assays, binding studies, functional assays, and as a positive control in Western blot or ELISA experiments
• Quality: Research-grade reagent manufactured under controlled conditions

For detailed specifications including molecular weight, purity grade, formulation buffer, storage conditions, and reconstitution instructions, please refer to the official product datasheet available on the Aladdin Scientific product page for SKU rp371132.

Research Applications of Recombinant Human GIMAP5 Protein

Recombinant Human GIMAP5 Protein is a versatile tool for researchers investigating immune system function and lymphocyte biology. Its applications span several key areas of modern biomedical research:

• T Cell Survival and Apoptosis Studies: GIMAP5 is a critical regulator of T lymphocyte survival. Recombinant GIMAP5 can be used to investigate pro-survival signaling pathways and apoptosis mechanisms in immune cells.
• Protein–Protein Interaction Analysis: As a GTPase family member, GIMAP5 participates in multi-protein complexes. Researchers can use recombinant GIMAP5 in co-immunoprecipitation, pull-down assays, or surface plasmon resonance studies to map its interactome.
• Autoimmunity and Immune Deficiency Research: Loss of GIMAP5 function has been linked to lymphopenia and autoimmune phenotypes in animal models. The recombinant protein supports mechanistic studies of these disease-relevant pathways.
• Assay Development and Validation: Recombinant GIMAP5 serves as a reliable positive control or calibration standard in immunoassays, including ELISA and Western blotting, where consistent protein reference material is required.
• Structural and Biochemical Studies: The protein can be employed in biochemical characterization assays to study GTPase activity, nucleotide binding, and structural conformation.

Handling, Storage, and Quality Considerations

Proper handling and storage of recombinant proteins are essential to maintaining biological activity and experimental reproducibility. The following general best practices apply to Recombinant Human GIMAP5 Protein:

• Storage: Recombinant proteins should generally be stored at –20°C or –80°C upon receipt to preserve activity. Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of function. Aliquoting the stock into single-use volumes is strongly recommended.
• Reconstitution: If supplied in lyophilized form, reconstitute the protein using the buffer and volume specified in the product datasheet. Allow the vial to equilibrate to room temperature before opening and reconstitute gently to avoid foaming or denaturation.
• Working Concentrations: Optimal working concentrations should be determined empirically for each specific assay. Titration experiments are advised prior to large-scale experiments.
• Quality Assurance: Each lot of Recombinant Human GIMAP5 Protein from Aladdin Scientific is subject to quality control testing. Refer to the Certificate of Analysis (CoA) for lot-specific purity, concentration, and activity data.

Always consult the product-specific datasheet and CoA, available through Aladdin Scientific customer support or the product page, for authoritative handling instructions.

Why Choose Aladdin Scientific for Recombinant Human GIMAP5 Protein?

Aladdin Scientific is committed to providing research scientists and laboratory professionals with high-quality, reliably sourced research reagents. Recombinant Human GIMAP5 Protein (rp371132) is offered as part of Aladdin's expanding portfolio of recombinant proteins supporting immunology, cell biology, and biochemistry research.

Key reasons to source GIMAP5 from Aladdin Scientific include:

• Rigorous Quality Control: Products are manufactured and tested under controlled conditions with lot-specific CoA documentation.
• Expert Technical Support: Aladdin Scientific's technical team is available to assist with product selection, reconstitution guidance, and troubleshooting for your specific research application.
• Competitive Pricing and Reliable Supply: Aladdin Scientific offers competitive pricing structures with a dependable supply chain to meet laboratory procurement requirements.
• Comprehensive Product Information: Detailed datasheets, safety data sheets (SDS), and CoAs are readily accessible to support regulatory compliance and experimental planning.

To order Recombinant Human GIMAP5 Protein or to request additional technical information, visit the Aladdin Scientific website or contact our customer support team.

Frequently asked questions

What is the biological function of GIMAP5 in the immune system?

GIMAP5 is a GTPase family protein primarily expressed in lymphocytes that acts as a pro-survival factor for T cells. It plays a key role in maintaining lymphocyte homeostasis and protecting T cells from apoptosis. Disruption of GIMAP5 function has been associated with lymphopenia and autoimmune-like phenotypes in animal models.

What applications is Recombinant Human GIMAP5 Protein suitable for?

Recombinant Human GIMAP5 Protein is suitable for a range of research applications including T cell survival and apoptosis studies, protein-protein interaction assays (e.g., co-immunoprecipitation, pull-down), immunoassay development and validation (Western blot, ELISA), and biochemical characterization of GTPase activity. It is also useful as a positive control in relevant immunological assays.

How should Recombinant Human GIMAP5 Protein be stored to maintain activity?

Recombinant Human GIMAP5 Protein should be stored at –20°C or –80°C upon receipt. To preserve activity and avoid protein degradation, repeated freeze-thaw cycles should be minimized by aliquoting the protein into single-use volumes before freezing. Always refer to the product-specific datasheet and Certificate of Analysis for authoritative storage and handling instructions.

Where can I find the Certificate of Analysis (CoA) for a specific lot of GIMAP5 protein?

The Certificate of Analysis for a specific lot of Recombinant Human GIMAP5 Protein (rp371132) can be obtained through the Aladdin Scientific product page or by contacting Aladdin Scientific customer support directly. The CoA provides lot-specific data including purity, concentration, and quality control results.

Is Recombinant Human GIMAP5 Protein suitable for use in animal studies or clinical applications?

Recombinant Human GIMAP5 Protein (rp371132) is supplied as a research-grade reagent intended exclusively for in vitro laboratory research use. It is not approved or validated for use in clinical diagnostics, therapeutic applications, or in vivo animal studies without appropriate validation and regulatory authorization.

References

1. Nitta T, Nasreen M, Seike T, et al. IAN family critically regulates survival and development of T lymphocytes. PLoS Biology. 2006.
2. Pandarpurkar M, Wilson-Fritch L, Corvera S, et al. Ian4 is required for mitochondrial integrity and T cell survival. Proceedings of the National Academy of Sciences. 2003.
3. Heinzel S, Dierich A, Bhatt D, et al. GIMAP family proteins and lymphocyte homeostasis. Immunological Reviews. 2010.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.