Recombinant Human GK5 Protein: Properties & Research Use

Recombinant Human GK5 Protein is a research-grade recombinant protein designed to support investigations into GK5 kinase function, metabolic signaling pathways, and related biochemical assays.

Product Name

Recombinant Human GK5 Protein

SKU

rp367951

Species Origin

Human (Homo sapiens)

Protein Type

Recombinant kinase-family protein

Intended Use

In vitro research use only; not for diagnostic or therapeutic use

Storage Recommendation

Follow supplier-provided datasheet guidelines; typically store at -20°C or -80°C, avoid repeated freeze-thaw cycles

Overview of GK5 and Its Biological Significance

GK5 (Glucokinase 5), also referred to as a member of the glucokinase-like kinase family, is a serine/threonine kinase implicated in cellular metabolic regulation and signaling networks. Members of this kinase family have been studied in the context of glucose metabolism, neuronal function, and intracellular signaling cascades. Understanding the precise biochemical role of GK5 at the molecular level is an active area of research.

Recombinant Human GK5 Protein (rp367951) from Aladdin Scientific provides researchers with a defined, research-grade reagent for characterizing GK5 enzymatic activity, protein–protein interactions, and downstream pathway effects under controlled in vitro conditions.

Recommended Handling and Storage Guidelines

Proper handling of recombinant proteins is critical to maintaining biological activity and experimental reproducibility. The following general guidelines apply to Recombinant Human GK5 Protein:

• Storage temperature: Consult the product-specific datasheet. Recombinant proteins are commonly stored at -20°C for short-term use or -80°C for long-term storage.
• Avoid freeze-thaw cycles: Repeated freeze-thaw cycles can denature the protein and reduce activity. Prepare single-use aliquots upon first thaw.
• Reconstitution: If lyophilized, reconstitute according to the datasheet instructions using the recommended buffer. Allow the protein to equilibrate to room temperature before use.
• Carrier protein: Adding BSA (bovine serum albumin) as a carrier protein may help stabilize diluted recombinant protein stocks.
• Contamination prevention: Use sterile, low-binding tubes and pipette tips to prevent adsorption losses and microbial contamination.

Always refer to the Aladdin Scientific product datasheet accompanying SKU rp367951 for the most specific and up-to-date handling instructions.

Research Applications

Recombinant Human GK5 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Enzyme activity assays: Characterize the kinase activity of GK5 toward candidate substrates using standard biochemical kinase assay formats (e.g., radiometric, fluorescent, or luminescent ATP-consumption assays).
• Protein interaction studies: Use GK5 as bait in pull-down or co-immunoprecipitation experiments to identify binding partners within metabolic or signaling complexes.
• Inhibitor screening: Employ recombinant GK5 in high-throughput or focused screens to identify small-molecule modulators of kinase activity.
• Structural and biophysical studies: Suitable as an antigen for antibody generation, as a standard in Western blot or ELISA, or as a component in biophysical binding studies (e.g., SPR, ITC).
• Pathway reconstitution: Incorporate into cell-free systems to study GK5-dependent signaling steps in metabolic or neuronal pathways.

Researchers should validate assay conditions empirically, including protein concentration, buffer composition, and co-factor requirements specific to their experimental system.

Quality, Purity, and Regulatory Considerations

Aladdin Scientific supplies Recombinant Human GK5 Protein (rp367951) as a research-grade reagent. Key quality considerations include:

• Purity: Recombinant proteins from Aladdin Scientific are produced and purified to research-grade standards. Specific purity data (e.g., SDS-PAGE purity percentage) are provided on the product datasheet.
• Formulation: Detailed formulation information, including buffer composition, glycerol content, and any stabilizing additives, is stated on the product-specific datasheet for rp367951.
• Endotoxin levels: Endotoxin content, where tested, will be indicated on the datasheet. For sensitive cell-based assays, confirm endotoxin specifications prior to use.
• Lot-to-lot consistency: Contact Aladdin Scientific technical support for lot-specific quality control data and certificates of analysis (CoA).

For Research Use Only (RUO). Not intended for use in diagnostic procedures or human/animal therapeutic applications.

Frequently asked questions

What is GK5 and why is it relevant to metabolic research?

GK5 is a member of the glucokinase-like kinase family, a group of serine/threonine kinases involved in cellular metabolism and signaling. Research into GK5 may provide insights into metabolic regulation and associated disease mechanisms. Recombinant Human GK5 Protein enables controlled in vitro studies of its biochemical functions.

How should I store Recombinant Human GK5 Protein (rp367951) to maintain activity?

Store the protein according to the instructions on the product datasheet provided with SKU rp367951. In general, recombinant proteins should be stored at -20°C for short-term use or -80°C for long-term storage, and repeated freeze-thaw cycles should be avoided by preparing single-use aliquots. Always consult the specific datasheet for authoritative storage conditions.

Is Recombinant Human GK5 Protein suitable for use in cell-based assays?

Recombinant Human GK5 Protein is primarily intended for in vitro biochemical assays. For cell-based applications, researchers should verify endotoxin levels from the product datasheet and confirm that the protein format and formulation are compatible with the intended assay system. Aladdin Scientific technical support can provide additional guidance.

Can Recombinant Human GK5 Protein be used as an antigen for antibody production?

Yes, recombinant proteins are commonly employed as antigens for generating research antibodies. Recombinant Human GK5 Protein may be suitable for this purpose; however, researchers should confirm purity, formulation, and immunogenicity suitability with the product datasheet and by consulting Aladdin Scientific technical support.

How do I obtain a Certificate of Analysis (CoA) for this product?

Certificates of Analysis for Recombinant Human GK5 Protein (rp367951) can be requested from Aladdin Scientific customer service or technical support, referencing the specific lot number of your product. CoA documents provide lot-specific purity, activity, and quality control data.

References

1. Manning G, Whyte DB, Martinez R, Hunter T, Sudarsanam S. The protein kinase complement of the human genome. Science. 2002;298(5600):1912-1934. doi:10.1126/science.1075762
2. Aladdin Scientific. Product Datasheet: Recombinant Human GK5 Protein, SKU rp367951. Aladdin Scientific; [date on datasheet].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.