Recombinant Human GLIPR1L1 Protein: Research Guide

Recombinant Human GLIPR1L1 Protein is a research-grade recombinant protein belonging to the GLIPR (GLI pathogenesis-related) family, offering scientists a well-characterized tool for investigating immune modulation, cell signaling, and cancer-associated biological pathways.

Protein Family

GLIPR (GLI Pathogenesis-Related) family, CRISP/PR-1/Sc7 superfamily

Species Origin

Human (Homo sapiens)

Product SKU

rp367968

Protein Type

Recombinant protein, research use only

Typical Applications

ELISA, Western blot, functional assays, protein interaction studies

Storage Recommendation

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles (verify with datasheet)

What Is GLIPR1L1? Protein Biology and Family Context

GLIPR1L1 (GLI Pathogenesis-Related 1-Like 1) is a member of the GLIPR subfamily within the broader CRISP/PR-1/Sc7 protein superfamily. Proteins in this superfamily are characterized by a conserved cysteine-rich domain and have been implicated in a range of biological processes, including innate immune defense, cell proliferation regulation, and apoptotic signaling.

GLIPR1L1 shares structural homology with GLIPR1 (also known as RTVP-1), a protein that has been extensively studied in the context of prostate cancer and glioblastoma. The GLIPR family members are thought to function at the interface of immune surveillance and tumor suppression, making them of significant interest in oncology and immunology research.

The gene encoding GLIPR1L1 is expressed in various human tissues, and its precise functional roles continue to be an active area of investigation. Research into GLIPR family proteins has highlighted their potential as biomarkers and as targets for therapeutic intervention in cancer biology.

Recombinant Production and Protein Specifications

Recombinant Human GLIPR1L1 Protein (SKU: rp367968) is produced using recombinant expression technology to ensure a well-defined, reproducible protein source suitable for in vitro research applications. Recombinant production eliminates the variability associated with native protein purification from tissue and allows for the generation of tag-fused or tag-free constructs depending on downstream experimental requirements.

Key quality attributes for recombinant proteins of this class typically include: high purity (generally >90% by SDS-PAGE), low endotoxin levels suitable for cell-based assays, defined molecular weight corresponding to the expressed sequence, and confirmed biological activity where applicable. Researchers are advised to consult the product-specific datasheet for confirmed purity, formulation, concentration, and activity data for lot rp367968.

The protein is supplied in a formulation optimized for stability during shipping and short-term storage. For long-term storage, aliquoting and storage at -80°C is generally recommended to preserve activity and minimize protein degradation.

Research Applications of Recombinant Human GLIPR1L1 Protein

Recombinant Human GLIPR1L1 Protein is a versatile research tool applicable across several experimental platforms commonly used in molecular and cell biology laboratories:

• ELISA and Immunoassays: The recombinant protein can serve as a standard or positive control antigen in sandwich or competitive ELISA formats, enabling quantification of GLIPR1L1-specific antibodies or measurement of protein levels in biological samples.
• Western Blot Validation: Purified recombinant GLIPR1L1 is useful as a positive control to validate antibody specificity and to optimize Western blot conditions before proceeding to complex sample matrices.
• Protein–Protein Interaction Studies: As a defined recombinant protein, GLIPR1L1 can be employed in pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance (SPR) experiments to map binding partners and characterize interaction kinetics.
• Functional and Cell-Based Assays: Exogenous addition of recombinant GLIPR1L1 to cell culture systems may be used to investigate its effects on cell viability, apoptosis, or immune cell activation, supporting mechanistic studies of GLIPR family function.
• Antibody Production and Screening: High-purity recombinant protein is an important immunogen source for generating and screening GLIPR1L1-specific antibodies for research or diagnostic applications.

Handling, Storage, and Quality Considerations

Proper handling of recombinant proteins is essential to preserve activity and ensure experimental reproducibility. The following general best practices apply to Recombinant Human GLIPR1L1 Protein and similar research-grade recombinant proteins:

Upon Receipt: Inspect the shipment immediately. Recombinant proteins are typically shipped on dry ice or with cold packs. If the product has thawed during transit, assess integrity before use and consult Aladdin Scientific technical support if in doubt.

Aliquoting: To minimize repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity, prepare single-use aliquots appropriate for your experimental needs upon first thaw.

Storage Conditions: Store aliquots at -80°C for long-term stability. Working stocks may be held at -20°C for short periods. Avoid storing at 4°C for extended durations unless the product datasheet specifically indicates this is acceptable.

Carrier Proteins and Stabilizers: Some recombinant protein formulations include BSA or other stabilizers. Confirm the formulation components from the product datasheet before use in functional assays where carrier proteins could interfere with results.

Quality Documentation: Aladdin Scientific provides lot-specific Certificates of Analysis (CoA). Always cross-reference the CoA for purity, concentration, and activity data before experimental use.

Frequently asked questions

What is GLIPR1L1 and why is it relevant to cancer research?

GLIPR1L1 is a member of the GLIPR protein family within the CRISP/PR-1/Sc7 superfamily, sharing structural features with GLIPR1/RTVP-1, a protein implicated in prostate cancer and glioma biology. Research into GLIPR family proteins suggests roles in tumor suppression, apoptosis, and immune modulation, making GLIPR1L1 a subject of interest for oncology and immunology studies. Its precise functional mechanisms are still under active investigation.

What applications is Recombinant Human GLIPR1L1 Protein suitable for?

Recombinant Human GLIPR1L1 Protein (SKU: rp367968) is suitable for multiple research applications including ELISA (as antigen standard or positive control), Western blot validation, protein–protein interaction studies (pull-down, SPR), cell-based functional assays, and as an immunogen for antibody generation and screening. Always verify suitability for your specific application by consulting the product datasheet.

How should Recombinant Human GLIPR1L1 Protein be stored to maintain stability?

For long-term storage, aliquot the protein into single-use volumes and store at -80°C to prevent degradation from repeated freeze-thaw cycles. Short-term storage at -20°C is acceptable for working stocks. Avoid prolonged storage at 4°C unless explicitly stated in the product datasheet. Always refer to the lot-specific Certificate of Analysis for the manufacturer's recommended storage conditions.

What purity level can I expect for Recombinant Human GLIPR1L1 Protein?

Research-grade recombinant proteins of this class are typically produced to a purity of greater than 90% as assessed by SDS-PAGE. However, exact purity values are lot-specific and should be confirmed by reviewing the Certificate of Analysis (CoA) provided by Aladdin Scientific for SKU rp367968.

References

1. Murphy EV, Zhang Y, Zhu W, Biggs J. A novel human glioma-associated oncogene homolog of Drosophila gli encodes a protein related to the Wilms tumor gene WT1. Nucleic Acids Research. 1994. [GLIPR family foundational reference — verify applicability to GLIPR1L1]
2. Gibbs GM, Roelants K, O'Bryan MK. The CAP superfamily: cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins — roles in reproduction, cancer, and immune defense. Endocrine Reviews. 2008;29(7):865-897. doi:10.1210/er.2008-0032
3. Ren C, Li L, Yang G, et al. RTVP-1, a tumor suppressor inactivated by methylation in prostate cancer. Cancer Research. 2004. [GLIPR1/RTVP-1 reference cited for family context — verify relevance to GLIPR1L1]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.