Recombinant Human GLIPR2 Protein: Key Facts & FAQs

Recombinant Human GLIPR2 Protein is a research-grade recombinant protein derived from the human GLIPR2 gene, offered by Aladdin Scientific (SKU: rp368113) to support studies in autophagy regulation, lipid binding, and related cellular signaling pathways.

Product Name

Recombinant Human GLIPR2 Protein

SKU

rp368113

Gene Name

GLIPR2 (also known as GAPR-1)

Protein Origin

Human (Homo sapiens)

Protein Family

CRISP/PR-1/Sc7 superfamily (CAP superfamily)

Primary Research Applications

Autophagy regulation, lipid-binding assays, protein interaction studies, cell signaling research

What Is GLIPR2 and Why Is It Scientifically Relevant?

GLIPR2 (Glioma Pathogenesis-Related Protein 2), also referred to as GAPR-1 (Golgi-Associated Plant Pathogenesis-Related Protein 1), is a member of the CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1) superfamily. It is a peripheral membrane protein that associates with the cytoplasmic leaflet of Golgi membranes through interactions with specific lipid species, including phosphatidylinositol.

GLIPR2 has attracted significant research interest due to its proposed role as a negative regulator of autophagy. It is thought to sequester Beclin-1, a key autophagy initiator, thereby modulating autophagic flux. Understanding GLIPR2 function is therefore relevant to research on cancer biology, neurodegenerative disease, and cellular stress responses.

Product Specifications and Quality Attributes

Recombinant Human GLIPR2 Protein (rp368113) from Aladdin Scientific is produced to research-grade standards. As with all recombinant proteins in this class, users should consult the product datasheet for confirmed details including:

• Expression system: Typically E. coli or mammalian/insect cell systems (verify on datasheet)
• Purity: Generally >90% as assessed by SDS-PAGE (verify on datasheet)
• Formulation: Supplied in a buffered solution; exact buffer composition and concentration are provided on the product datasheet
• Endotoxin level: Determined by LAL assay; specific threshold listed on datasheet
• Molecular weight: Corresponding to the predicted molecular weight of human GLIPR2, confirm with datasheet for any tag additions

Always refer to the official Aladdin Scientific product datasheet for rp368113 for the most accurate and up-to-date specifications prior to experimental use.

Storage, Handling, and Stability Guidelines

Proper storage and handling of Recombinant Human GLIPR2 Protein are essential to maintain its biological activity and structural integrity. General best-practice guidelines for recombinant proteins of this type include:

• Short-term storage: Store at 4°C if the protein will be used within a few days of receipt.
• Long-term storage: Aliquot and store at -20°C or -80°C to avoid repeated freeze-thaw cycles, which can reduce activity.
• Reconstitution: If lyophilized, reconstitute with the recommended volume of sterile water or the buffer specified on the datasheet; allow to dissolve gently without vortexing.
• Carrier protein: Adding a carrier protein such as 0.1% HSA or BSA may enhance stability during storage.
• Avoid repeated freeze-thaw: Single-use aliquots are strongly recommended.

Specific storage conditions for rp368113 are detailed on the official product datasheet. Always prioritize datasheet instructions over general guidelines.

Common Research Applications of GLIPR2 Protein

Recombinant Human GLIPR2 Protein is used across a range of in vitro and biochemical research applications, including but not limited to:

• Autophagy studies: Investigating GLIPR2's interaction with Beclin-1 and its role in modulating autophagic initiation.
• Lipid-binding assays: Characterizing GLIPR2's affinity for phosphoinositides and other membrane lipids.
• Protein–protein interaction (PPI) studies: Co-immunoprecipitation, pull-down, or surface plasmon resonance (SPR) assays to map GLIPR2 binding partners.
• Structural biology: As a standard for crystallography or cryo-EM studies of the CAP superfamily.
• Antibody validation: As a positive control antigen for validating anti-GLIPR2 antibodies in Western blot or ELISA formats.
• Cancer biology research: Examining GLIPR2 expression changes and their functional consequences in tumor cell lines.

Frequently asked questions

What is GLIPR2 protein and what is its primary biological function?

GLIPR2 (also known as GAPR-1) is a member of the CAP superfamily and functions as a peripheral Golgi membrane-associated protein. Its primary studied role is as a negative regulator of autophagy, where it is proposed to interact with Beclin-1 to suppress autophagic initiation. It also exhibits lipid-binding properties relevant to membrane association.

How should Recombinant Human GLIPR2 Protein (rp368113) be stored to maintain activity?

For short-term use within a few days, storage at 4°C is generally acceptable. For long-term storage, aliquot the protein and store at -20°C or -80°C to prevent activity loss from repeated freeze-thaw cycles. Always consult the official product datasheet for rp368113 for the precise recommended storage conditions and formulation details.

What expression system is used to produce Recombinant Human GLIPR2 Protein (rp368113)?

The specific expression system used for rp368113 is detailed on the Aladdin Scientific product datasheet. Common systems for recombinant proteins of this type include E. coli, mammalian, or insect cell expression platforms. Please refer to the datasheet or contact Aladdin Scientific technical support for confirmed information.

Can Recombinant Human GLIPR2 Protein be used as a positive control in Western blot or ELISA?

Yes, recombinant proteins such as GLIPR2 (rp368113) are commonly used as positive control antigens to validate the specificity and sensitivity of anti-GLIPR2 antibodies in immunoassay formats including Western blot and ELISA. Ensure that the molecular weight and tag (if any) are compatible with your detection antibody before use.

What purity level can I expect from Recombinant Human GLIPR2 Protein (rp368113)?

Research-grade recombinant proteins are typically supplied at greater than 90% purity as assessed by SDS-PAGE. However, the confirmed purity specification for rp368113 is stated on the official product datasheet. Please review the datasheet or contact Aladdin Scientific for the precise purity value and QC data.

References

1. Eberle HB, et al. Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex. Journal of Cell Science. 2002.
2. Shoji-Kawata S, et al. Identification of a candidate therapeutic autophagy-inducing peptide. Nature. 2013.
3. Aladdin Scientific. Recombinant Human GLIPR2 Protein, SKU rp368113. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.