Recombinant Human GLOD5 Protein: Properties & Applications

Recombinant Human GLOD5 Protein is a recombinantly expressed form of the human glyoxalase domain-containing protein 5, produced for use in biochemical assays, structural studies, and investigations of metabolic pathways.

Product Name

Recombinant Human GLOD5 Protein

SKU

rp376161

Gene Name

GLOD5 (Glyoxalase Domain Containing 5)

Species Origin

Human (Homo sapiens)

Protein Form

Recombinant

Primary Application

Biochemical assays, protein interaction studies, metabolic research

What Is GLOD5?

GLOD5, or Glyoxalase Domain Containing Protein 5, is a member of the glyoxalase superfamily — a group of enzymes characterized by a conserved beta-alpha-beta structural motif that typically catalyzes reactions involving glutathione-dependent conversion of reactive carbonyl compounds. The GLOD5 gene encodes a protein that shares structural homology with classical glyoxalases, which are involved in the detoxification of methylglyoxal and other cytotoxic byproducts of glycolysis.

While the precise enzymatic function of GLOD5 in human physiology remains an area of active investigation, its domain architecture suggests a role in cellular metabolic regulation and stress response. Research into glyoxalase superfamily members has gained momentum given their implications in diabetes, cancer metabolism, and aging.

Production and Biochemical Characteristics

Recombinant Human GLOD5 Protein (SKU: rp376161) is produced using recombinant expression systems to ensure consistent batch-to-batch quality and high purity suitable for research applications. Recombinant production allows researchers to obtain defined quantities of the protein without dependence on tissue extraction, thereby minimizing variability associated with biological sourcing.

Key characteristics of recombinant proteins like GLOD5 include defined amino acid sequence fidelity to the human gene, controlled post-translational modification profiles depending on the expression host, and suitability for a range of downstream assays. Researchers should consult the product datasheet for specific details on purity grade, endotoxin levels, formulation buffer, and recommended storage conditions.

Research Applications

Recombinant Human GLOD5 Protein is suitable for a variety of research applications, including but not limited to:

• Biochemical assays: Activity assays and substrate profiling to characterize the enzymatic properties of GLOD5.
• Protein–protein interaction studies: Co-immunoprecipitation, pull-down assays, and surface plasmon resonance to identify binding partners.
• Structural biology: Crystallography and cryo-EM studies to determine the three-dimensional structure of GLOD5 and its complexes.
• Antibody development and validation: As an antigen for generating or validating GLOD5-specific antibodies.
• Metabolic pathway research: Investigating the role of GLOD5 in carbonyl stress and metabolic disease models.

Given the protein's membership in the glyoxalase superfamily, it is of particular interest in the context of diabetes, neurodegenerative diseases, and oncology research where methylglyoxal detoxification pathways are implicated.

Handling, Storage, and Quality Considerations

Proper handling and storage are critical to maintaining the activity and integrity of recombinant proteins. General best practices for Recombinant Human GLOD5 Protein include:

• Storage: Store at –20°C or –80°C as recommended on the product datasheet. Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.
• Aliquoting: Upon first use, prepare single-use aliquots to minimize freeze-thaw events.
• Reconstitution: Follow the datasheet instructions for reconstitution buffer and concentration. Centrifuge briefly before opening to consolidate lyophilized material if applicable.
• Quality verification: Confirm protein integrity by SDS-PAGE or Western blot prior to use in critical assays.

Researchers should always consult the current product datasheet provided by Aladdin Scientific for lot-specific information on purity, concentration, and recommended applications.

Frequently asked questions

What is the gene function of GLOD5?

GLOD5 encodes a glyoxalase domain-containing protein that is structurally homologous to classical glyoxalase enzymes involved in detoxification of reactive carbonyl species such as methylglyoxal. Its precise physiological function in humans is still under investigation.

What expression system is used to produce Recombinant Human GLOD5 Protein?

The specific expression system used for rp376161 should be confirmed via the product datasheet available from Aladdin Scientific, as expression host details (e.g., E. coli, HEK293, baculovirus) directly affect post-translational modifications and protein functionality.

How should Recombinant Human GLOD5 Protein be stored to maintain stability?

It is generally recommended to store recombinant proteins at –20°C or –80°C, prepare single-use aliquots to avoid repeated freeze-thaw cycles, and follow the specific storage and reconstitution instructions provided in the Aladdin Scientific product datasheet for rp376161.

What research applications is Recombinant Human GLOD5 Protein suitable for?

Recombinant Human GLOD5 Protein is suitable for biochemical activity assays, protein–protein interaction studies, structural biology investigations, antibody development and validation, and metabolic pathway research, particularly studies related to carbonyl stress and glyoxalase superfamily biology.

Is Recombinant Human GLOD5 Protein suitable for use as an ELISA standard or Western blot control?

Recombinant human proteins are commonly used as positive controls in Western blotting and as calibration standards in ELISA assays. Researchers should verify suitability for these specific applications by consulting the product datasheet or contacting Aladdin Scientific technical support.

References

1. Thornalley PJ. The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life. Biochem J. 1990;269(1):1–11.
2. Rabbani N, Thornalley PJ. Methylglyoxal, glyoxalase 1 and the dicarbonyl proteome. Amino Acids. 2012;42(4):1133–1142.
3. Aladdin Scientific. Recombinant Human GLOD5 Protein, SKU rp376161. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.