Recombinant Human GOLGA6L9 Protein: Research Guide

Recombinant Human GOLGA6L9 Protein is a research-grade recombinant protein derived from the human GOLGA6L9 gene, belonging to the Golgin family of coiled-coil Golgi structural proteins, and is available from Aladdin Scientific (SKU: rp377474) to support studies in Golgi apparatus biology and related cellular processes.

Product Name

Recombinant Human GOLGA6L9 Protein

SKU

rp377474

Protein Family

Golgin family (coiled-coil Golgi matrix proteins)

Species Origin

Human (Homo sapiens)

Application Area

Golgi apparatus biology, cell biology, protein interaction studies

Format

Recombinant protein (refer to product datasheet for specific formulation details)

Supplier

Aladdin Scientific

Background: GOLGA6L9 and the Golgin Protein Family

GOLGA6L9 (Golgin A6 Family-Like 9) is a member of the extended Golgin superfamily, a group of predominantly coiled-coil proteins that are localized at or near the Golgi apparatus. Golgins are well recognized for their roles in maintaining Golgi morphology, facilitating vesicular trafficking, and tethering transport vesicles to appropriate Golgi compartments.

The GOLGA6 subfamily and its paralogs, including GOLGA6L9, are encoded by genes on human chromosome 15 and represent members of a rapidly evolving gene family that arose through segmental duplications. While the precise biochemical functions of GOLGA6L9 remain an active area of investigation, its structural features — including extended coiled-coil domains — are consistent with roles in Golgi tethering or scaffolding functions shared by other Golgin family members.

Research into Golgin proteins has broad relevance for understanding intracellular membrane trafficking, organelle biogenesis, and diseases associated with Golgi dysfunction, including certain congenital disorders of glycosylation and neurodevelopmental conditions.

Product Specifications and Quality Attributes

Recombinant Human GOLGA6L9 Protein (rp377474) is produced using recombinant expression technology to deliver a research-grade protein suitable for in vitro biochemical and cell biology experiments. Researchers should consult the product-specific datasheet available from Aladdin Scientific for confirmed details on the following attributes, as these may vary by lot:

• Expression system: Refer to product datasheet (commonly E. coli, insect cell, or mammalian expression systems are used for Golgin family proteins)
• Protein purity: Refer to product datasheet for purity specification and SDS-PAGE verification data
• Tag information: Refer to product datasheet for any affinity or epitope tags present
• Formulation and buffer: Refer to product datasheet for buffer composition and storage excipients
• Biological activity: Refer to product datasheet for activity data where applicable

All Aladdin Scientific recombinant proteins undergo quality control testing prior to release. Lot-specific certificates of analysis (CoA) are available upon request.

Recommended Applications

Recombinant Human GOLGA6L9 Protein is intended for research use only and is suitable for a range of in vitro applications. Typical applications for recombinant Golgin family proteins include:

• SDS-PAGE and Western Blot: Use as a positive control or molecular weight standard for antibody validation experiments targeting GOLGA6L9.
• ELISA: Use as a coating or standard antigen in sandwich or competitive ELISA formats.
• Protein–protein interaction studies: Pull-down assays or surface plasmon resonance (SPR) experiments to identify binding partners of GOLGA6L9 within Golgi tethering complexes.
• Antibody production and screening: Immunization antigen for generating anti-GOLGA6L9 antibodies or for screening hybridoma supernatants.
• Structural and biophysical studies: Circular dichroism (CD), dynamic light scattering (DLS), or cryo-EM sample preparation where protein purity and homogeneity are critical.

Researchers are advised to optimize protein concentration, buffer conditions, and assay formats empirically for each specific experimental application.

Storage, Handling, and Stability Guidelines

Proper storage and handling of recombinant proteins are essential to preserve activity and prevent degradation. The following general best practices apply to Recombinant Human GOLGA6L9 Protein; always defer to the product-specific datasheet for confirmed conditions:

• Storage temperature: Recombinant proteins are typically stored at −20 °C or −80 °C for long-term stability. Avoid repeated freeze–thaw cycles, which can cause aggregation and loss of activity.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize freeze–thaw events. Store aliquots at −80 °C unless the datasheet specifies otherwise.
• Working conditions: Keep protein on ice during experimental setup. Avoid prolonged exposure to ambient temperature or proteolytic contamination.
• Carrier protein: If the protein is provided in a low-concentration or carrier-free format, consider adding BSA (0.1–0.5%) as a stabilizing agent during dilution, unless incompatible with downstream assays.
• Expiry and lot tracking: Note the lot number and expiry date from the CoA. Do not use expired product in quantitative assays.

Contact Aladdin Scientific technical support if stability concerns arise or if the protein does not perform as expected in validated assay formats.

Ordering Information and Technical Support

Recombinant Human GOLGA6L9 Protein is available through Aladdin Scientific under SKU rp377474. To place an order or request a quotation, contact your regional Aladdin Scientific sales representative or visit the Aladdin Scientific website.

For technical questions regarding experimental applications, buffer compatibility, or custom formulation requests, Aladdin Scientific's technical support team is available to assist. When contacting support, please have the following information ready:

• Product SKU and lot number
• Intended application and assay format
• Buffer and storage conditions used
• Description of the issue or question

Bulk quantities, custom concentrations, or alternative formulations may be available upon request. All products are intended for research use only and are not for diagnostic or therapeutic purposes.

Frequently asked questions

What is GOLGA6L9 and why is it scientifically relevant?

GOLGA6L9 (Golgin A6 Family-Like 9) is a member of the Golgin superfamily of coiled-coil proteins associated with the Golgi apparatus. Golgins are critically involved in maintaining Golgi structure, vesicular tethering, and intracellular membrane trafficking. GOLGA6L9 is encoded by a gene on human chromosome 15 within a region subject to segmental duplication, making it relevant to genomic stability research as well as Golgi biology.

What applications is Recombinant Human GOLGA6L9 Protein (rp377474) validated for?

Typical applications for this recombinant protein include use as a positive control in Western blot and ELISA, as an immunogen for antibody generation, and in protein–protein interaction assays such as pull-down experiments. Researchers should consult the product datasheet for application-specific validation data, as confirmed activities may vary by lot.

How should Recombinant Human GOLGA6L9 Protein be stored to maintain stability?

For long-term storage, the protein should be kept at −80 °C and repeated freeze–thaw cycles should be avoided. Upon first use, prepare single-use aliquots before refreezing. Always refer to the product datasheet for the specific storage buffer and stability data for each lot, as formulation details are lot-dependent.

Is Recombinant Human GOLGA6L9 Protein suitable for in vivo or clinical use?

No. Recombinant Human GOLGA6L9 Protein (rp377474) from Aladdin Scientific is intended for research use only. It is not approved, validated, or manufactured to standards required for in vivo administration, diagnostic testing, or therapeutic applications.

How can I request a Certificate of Analysis (CoA) for a specific lot of rp377474?

Lot-specific Certificates of Analysis for Recombinant Human GOLGA6L9 Protein (rp377474) can be requested by contacting Aladdin Scientific customer service or technical support. Please provide your order number and lot number when making the request to ensure the correct documentation is issued.

References

1. Munro S. The golgin coiled-coil proteins of the Golgi apparatus. Cold Spring Harb Perspect Biol. 2011;3(6):a005256. doi:10.1101/cshperspect.a005256
2. Barr FA, Short B. Golgins in the structure and dynamics of the Golgi apparatus. Curr Opin Cell Biol. 2003;15(4):405-413. doi:10.1016/S0955-0674(03)00054-1
3. Sudmant PH, et al. Diversity of human copy number variation and multicopy genes. Science. 2010;330(6004):641-646. doi:10.1126/science.1197005

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.