Recombinant Human GOLGA8Q Protein: Research Guide

Recombinant Human GOLGA8Q Protein is a research-grade recombinant protein derived from the human GOLGA8Q gene, belonging to the Golgin family of Golgi-associated coiled-coil proteins, and is offered by Aladdin Scientific (SKU: rp377484) to support investigations into Golgi apparatus structure, membrane trafficking, and related cellular processes.

Product Name

Recombinant Human GOLGA8Q Protein

SKU

rp377484

Protein Family

Golgin family (Golgi-associated coiled-coil proteins)

Species

Human (Homo sapiens)

Application Area

Golgi biology, membrane trafficking, cell biology research

Product Type

Recombinant Protein

Background: The GOLGA8Q Gene and Golgin Protein Family

GOLGA8Q (Golgin A8 Family Member Q) is a member of the Golgin superfamily, a diverse group of coiled-coil proteins that are predominantly localized to the Golgi apparatus. Golgins are characterized by extensive coiled-coil domains that facilitate protein–protein interactions and are essential for maintaining the structural integrity of the Golgi stack, regulating vesicular trafficking, and coordinating the sorting of cargo through the secretory pathway.

The GOLGA8 subfamily, to which GOLGA8Q belongs, is notable for being encoded within a large segmental duplication region on human chromosome 15q13-q14. Members of this subfamily share significant sequence homology, making their individual functional characterization a subject of active research. Understanding the specific roles of individual GOLGA8 family members, including GOLGA8Q, requires recombinant protein tools that enable controlled biochemical and cell biological experiments.

Protein Properties and Production

Recombinant Human GOLGA8Q Protein (SKU: rp377484) is produced using recombinant DNA technology to yield a protein that reflects the human GOLGA8Q sequence. Recombinant production systems — which may include bacterial, insect, or mammalian expression platforms — allow for the generation of defined, reproducible protein material suitable for use in controlled laboratory settings.

Key physicochemical features characteristic of Golgin-family proteins include extended coiled-coil secondary structures, which confer an elongated molecular architecture. These structural properties underpin the protein's ability to tether vesicles and interact with Rab GTPases and other trafficking regulators at the Golgi membrane.

Note: Detailed specifications including exact molecular weight, expression host, purity grade, formulation buffer, and recommended storage conditions should be confirmed by consulting the official Aladdin Scientific product datasheet for rp377484.

Research Applications

Recombinant Human GOLGA8Q Protein is suitable for a range of research applications focused on Golgi apparatus biology and intracellular membrane trafficking. Typical applications for recombinant Golgin-family proteins include:

• Biochemical Interaction Studies: Co-immunoprecipitation, pull-down assays, and surface plasmon resonance experiments to identify binding partners and map interaction domains.
• Structural Studies: Use as an antigen for antibody generation or as a reference standard in Western blotting and ELISA development.
• Functional Reconstitution Assays: In vitro vesicle tethering and fusion assays to dissect the mechanistic contributions of GOLGA8Q to Golgi trafficking.
• Disease Model Research: Investigation of the potential involvement of chromosome 15q13 copy number variation-associated proteins in neurological or developmental conditions.

Researchers should validate the protein for each specific application and refer to the product datasheet for confirmed application data.

Handling, Storage, and Quality Considerations

Proper handling and storage of recombinant proteins are critical to maintaining activity and ensuring experimental reproducibility. General best practices for recombinant proteins of this type include:

• Upon receipt, briefly centrifuge the vial before opening to consolidate the contents.
• Reconstitute lyophilized protein in the recommended buffer as specified in the product datasheet; avoid vigorous vortexing.
• Aliquot the reconstituted protein to minimize freeze-thaw cycles, which can lead to aggregation and loss of activity.
• Store aliquots at –20°C or –80°C as recommended; avoid repeated freeze-thaw cycles.
• Assess protein integrity by SDS-PAGE or Western blot prior to use in critical experiments.

Quality assurance: Aladdin Scientific recombinant proteins undergo quality control testing. Specific QC data — including purity assessment (typically by SDS-PAGE), endotoxin levels, and biological activity (where applicable) — are documented on the individual product Certificate of Analysis (CoA). Contact Aladdin Scientific technical support for CoA information for lot rp377484.

Frequently asked questions

What is the biological function of GOLGA8Q?

GOLGA8Q is a member of the Golgin A8 subfamily of Golgi-associated coiled-coil proteins. Golgins in general play roles in maintaining Golgi structure, vesicle tethering, and intracellular membrane trafficking. The specific functional role of GOLGA8Q among the highly homologous GOLGA8 family members remains an active area of investigation, and recombinant protein tools like rp377484 are valuable for elucidating its distinct contributions.

What expression system is used to produce Recombinant Human GOLGA8Q Protein (rp377484)?

The specific expression host and production system for rp377484 should be confirmed by consulting the Aladdin Scientific product datasheet or Certificate of Analysis. Common expression systems for recombinant human proteins include E. coli, baculovirus/insect cell, and mammalian cell platforms, each offering different trade-offs in terms of post-translational modification and protein folding.

What quality control testing is performed on this recombinant protein?

Aladdin Scientific performs quality control testing on recombinant proteins, which typically includes purity assessment by SDS-PAGE, and may also include endotoxin testing and functional activity assays where applicable. Specific QC data for rp377484 are detailed in the product Certificate of Analysis (CoA), which can be requested from Aladdin Scientific technical support.

How should Recombinant Human GOLGA8Q Protein be stored to maintain stability?

General best practices for recombinant protein storage include storing aliquots at –20°C or –80°C to minimize degradation, and avoiding repeated freeze-thaw cycles. Specific storage conditions and recommended buffer formulations for rp377484 are provided in the official product datasheet. Always follow the datasheet instructions for optimal protein stability.

References

1. Munro S. The golgin coiled-coil proteins of the Golgi apparatus. Cold Spring Harb Perspect Biol. 2011;3(6):a005256. doi:10.1101/cshperspect.a005256
2. Gillingham AK, Munro S. Long coiled-coil proteins and membrane traffic. Biochim Biophys Acta. 2003;1641(2-3):71-85. doi:10.1016/S0167-4889(03)00088-0
3. Antonacci F, et al. Characterization of six human disease-associated inversion polymorphisms. Hum Mol Genet. 2009;18(14):2555-2566. doi:10.1093/hmg/ddp187

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.