Recombinant Human GPA33 Protein: Properties & Research Guide

Recombinant Human GPA33 Protein is a recombinantly produced form of the GPA33 cell-surface antigen, a glycoprotein highly expressed in the gastrointestinal epithelium and widely studied as a colorectal cancer biomarker and therapeutic target.

Product Name

Recombinant Human GPA33 Protein

SKU

rp368030

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant cell-surface glycoprotein antigen

Primary Research Area

Oncology, gastrointestinal biology, immunotherapy

Key Application

Biomarker research, antibody validation, binding assays

What Is GPA33 and Why Does It Matter?

GPA33 (Glycoprotein A33) is a type I transmembrane protein belonging to the immunoglobulin superfamily. It is predominantly expressed on the surface of intestinal epithelial cells throughout the gastrointestinal tract and is retained in the majority of colorectal carcinomas, making it a clinically relevant biomarker and therapeutic target.

Because GPA33 expression is largely restricted to the gastrointestinal epithelium and colorectal tumors, it has attracted significant attention as a target for antibody-based diagnostics and immunotherapeutic strategies, including radioimmunotherapy and antibody-drug conjugates (ADCs). Researchers have employed anti-GPA33 antibodies in clinical trials for colorectal cancer, underscoring the translational importance of this antigen.

Recommended Experimental Applications

Recombinant Human GPA33 Protein (rp368030) is suitable for a range of in vitro research applications:

• Antibody Validation: Use as a positive control antigen to validate anti-GPA33 antibodies in ELISA, Western blot, and surface plasmon resonance (SPR) assays.
• Binding and Inhibition Assays: Employ in ligand-binding or competitive inhibition studies to characterize molecules that interact with the GPA33 extracellular domain.
• Biomarker Research: Utilize as a reference standard in assay development for detection of GPA33 in biological samples.
• Immunotherapy Research: Apply in preclinical evaluation of GPA33-targeting therapeutic constructs such as bispecific antibodies or ADC payloads.
• Cell Biology Studies: Support functional studies examining GPA33's role in epithelial cell adhesion and intestinal homeostasis.

Researchers should perform application-specific optimization, including titration of protein concentration, buffer conditions, and assay format, prior to use in definitive experiments.

Storage, Handling, and Stability Guidelines

Proper handling of Recombinant Human GPA33 Protein is essential to maintain biological activity and experimental reproducibility. The following general guidelines apply to recombinant proteins of this class:

• Storage Temperature: Store lyophilized protein at -20 °C or -80 °C for long-term stability. Reconstituted protein should be aliquoted to avoid repeated freeze-thaw cycles.
• Reconstitution: Reconstitute lyophilized protein in sterile, deionized water or the buffer specified in the product datasheet. Allow to equilibrate to room temperature before use.
• Freeze-Thaw Cycles: Minimize freeze-thaw cycles to no more than 2-3 times to prevent protein degradation and loss of activity.
• Carrier Protein: If storing at low concentrations (<10 µg/mL), consider adding a carrier protein such as 0.1% BSA to stabilize the protein.
• Contamination Prevention: Use aseptic technique at all times. Do not use protein from vials showing signs of contamination or turbidity.

Always consult the product datasheet (SKU: rp368030) for lot-specific storage conditions, concentration, and formulation details.

Quality Assurance and Purity Considerations

When selecting a recombinant protein for critical research applications, quality attributes should be carefully evaluated. Key considerations for Recombinant Human GPA33 Protein include:

• Purity: High-purity preparations are essential for quantitative assays and immunological studies. Confirm purity by SDS-PAGE or HPLC as documented in the certificate of analysis (CoA).
• Endotoxin Level: For cell-based assays and in vivo studies, endotoxin content should be verified and minimized. Check the CoA for LAL-tested endotoxin values.
• Biological Activity: Where applicable, functional activity data (e.g., binding affinity, receptor activation) should be confirmed per the product datasheet.
• Glycosylation: As a native glycoprotein, GPA33 carries N-linked glycans. Depending on the expression system used (e.g., HEK293, CHO, E. coli), glycosylation patterns may differ from native human protein. Researchers should consider this when interpreting results from immunological or binding assays.

Contact Aladdin Scientific technical support for lot-specific quality documentation (CoA) for SKU rp368030.

Frequently asked questions

What expression system is used to produce Recombinant Human GPA33 Protein (rp368030)?

The specific expression system for rp368030 should be confirmed by consulting the product datasheet or contacting Aladdin Scientific technical support. Common expression systems for glycoproteins like GPA33 include mammalian systems (HEK293, CHO) to preserve native-like glycosylation, though E. coli systems may also be used for non-glycosylated forms.

Is Recombinant Human GPA33 Protein suitable for use in cell-based functional assays?

Recombinant Human GPA33 Protein can be used in cell-based assays provided that endotoxin levels are within acceptable limits and the protein formulation is compatible with cell culture conditions. Researchers should validate protein activity and confirm absence of cytotoxic contaminants before use in functional cellular experiments.

How should I reconstitute and store Recombinant Human GPA33 Protein after opening?

Reconstitute lyophilized GPA33 protein in sterile deionized water or the buffer recommended in the product datasheet. After reconstitution, aliquot the protein into single-use volumes and store at -80 °C to avoid repeated freeze-thaw cycles. Refer to the product datasheet for rp368030 for lot-specific reconstitution volume and formulation recommendations.

Can Recombinant Human GPA33 Protein be used as an immunogen for antibody generation?

Recombinant human proteins are commonly employed as immunogens for antibody generation. Recombinant Human GPA33 Protein may be suitable for this purpose; however, researchers should assess protein purity, integrity, and whether the glycosylation profile is appropriate for the intended immune response. Consult Aladdin Scientific technical support for guidance specific to rp368030.

References

1. Heath JK, et al. The human A33 antigen is a transmembrane glycoprotein and a novel member of the immunoglobulin superfamily. Proceedings of the National Academy of Sciences USA. 1997;94(2):469-474.
2. Welt S, et al. Phase I study of anticolon cancer humanized antibody A33. Clinical Cancer Research. 2003;9(4):1338-1346.
3. Ritter G, et al. The A33 antigen: a novel target for antibody-based therapy of colorectal cancer. Clinical Cancer Research. 1997;3(8):1493-1502.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.