Recombinant Human GPATCH11 Protein: Key FAQs

Recombinant Human GPATCH11 Protein is a recombinantly expressed form of the human G-patch domain-containing protein 11, produced for use in biochemical, structural, and functional research applications.

Product Name

Recombinant Human GPATCH11 Protein

SKU

rp375062

Protein Family

G-patch domain-containing proteins

Species Origin

Human (Homo sapiens)

Primary Research Use

Biochemical assays, protein interaction studies, functional characterization

Storage Recommendation

Follow supplier-provided datasheet; typically -20°C or -80°C; avoid repeated freeze-thaw cycles

What Is GPATCH11 and Why Is It Researched?

GPATCH11, also known as G-patch domain-containing protein 11, is a member of the G-patch domain protein family. G-patch domains are conserved glycine-rich motifs found in a variety of eukaryotic proteins and are known to play roles in RNA processing, including pre-mRNA splicing. GPATCH11 has been implicated in the regulation of RNA helicase activity and splicing factor function, making it a subject of interest in studies related to RNA metabolism and gene expression regulation.

Research into GPATCH11 may contribute to a broader understanding of post-transcriptional gene regulation and its potential links to disease mechanisms, including roles in developmental and cellular homeostasis pathways. The recombinant form of this protein enables controlled in vitro experimentation not easily achieved with endogenous protein sources.

What Are the Typical Applications for Recombinant Human GPATCH11 Protein?

Recombinant Human GPATCH11 Protein is suited for a range of in vitro research applications, including but not limited to:

• Protein–protein interaction studies: Investigating binding partners of GPATCH11, particularly in the context of RNA helicase complexes and spliceosomal components.
• Biochemical activity assays: Evaluating the modulatory effects of GPATCH11 on associated enzyme activities.
• Antibody validation: Serving as a positive control antigen for validating antibodies raised against GPATCH11.
• Structural studies: Providing purified protein for biophysical characterization methods such as SPR, ITC, or crystallography.
• Functional reconstitution experiments: Reconstituting partial RNA processing complexes in defined in vitro systems.

Researchers should consult the product datasheet for validated applications specific to this lot and expression system.

How Should Recombinant Human GPATCH11 Protein Be Stored and Handled?

Proper storage and handling are critical to maintaining the activity and integrity of recombinant proteins. General best-practice guidelines for recombinant proteins like GPATCH11 include:

• Temperature: Store at -20°C for short-term use or -80°C for long-term storage, as specified on the product datasheet.
• Aliquoting: Upon first thaw, aliquot the protein into single-use volumes to prevent activity loss from repeated freeze-thaw cycles.
• Carrier protein: If the protein is supplied without a stabilizing carrier, consider adding BSA to a final concentration of 0.1% to improve stability during storage.
• Avoid repeated freeze-thaw: Each freeze-thaw cycle can lead to protein aggregation and loss of functional activity.
• Working conditions: Keep protein on ice during experimental setup and minimize exposure to room temperature.

Always refer to the specific lot datasheet provided with the product for exact storage conditions and expiry information, as formulations may vary.

What Quality and Purity Standards Apply to This Recombinant Protein?

Recombinant proteins intended for research use are typically evaluated against several quality parameters to ensure reproducibility and reliability of experimental results. For Recombinant Human GPATCH11 Protein, the following quality attributes are generally assessed:

• Purity: Assessed by SDS-PAGE and/or HPLC; typical research-grade recombinant proteins target ≥85–95% purity, though the exact specification should be confirmed on the product datasheet.
• Identity confirmation: Verified by Western blot using GPATCH11-specific antibodies or by mass spectrometry.
• Endotoxin levels: Tested by LAL assay; low endotoxin specifications are important for cell-based assays.
• Bioactivity: Functional activity may be validated through binding assays or co-factor stimulation assays depending on known biology.

Researchers requiring lot-specific certificates of analysis (CoA) should contact Aladdin Scientific customer service or request documentation at the time of ordering.

Frequently asked questions

What is the difference between GPATCH11 and other G-patch domain proteins?

GPATCH11 is one of several human proteins harboring the G-patch domain, a conserved glycine-rich motif associated with RNA binding and modulation of RNA helicases. While G-patch domain proteins share this structural feature, each member—including GPATCH11—has a distinct domain architecture, expression pattern, and set of interacting partners, conferring unique biological roles. Researchers should consult current literature for the latest functional characterization of GPATCH11 relative to paralogs such as GPATCH2, GPATCH4, or RBM5.

Is Recombinant Human GPATCH11 Protein suitable for cell-based assays?

Recombinant Human GPATCH11 Protein can be used in cell-based contexts if endotoxin levels meet the required thresholds for the intended cell type, as high endotoxin contamination can confound cellular responses. Researchers should verify the endotoxin specification on the product datasheet and perform a cell viability control when introducing exogenous protein into culture systems. Delivery method (e.g., protein transfection reagents) may also need to be optimized for intracellular targets like GPATCH11.

How do I confirm the identity of the recombinant GPATCH11 protein upon receipt?

Upon receipt, protein identity can be confirmed by SDS-PAGE to verify the apparent molecular weight band, followed by Western blot using a validated anti-GPATCH11 antibody. For additional confirmation, mass spectrometry-based peptide mapping provides definitive sequence verification. Aladdin Scientific provides a Certificate of Analysis (CoA) with each product lot; researchers should cross-reference their in-house QC results with the CoA data supplied.

What expression system is used to produce Recombinant Human GPATCH11 Protein?

The specific expression system (e.g., E. coli, baculovirus/insect cells, mammalian cells) used for Recombinant Human GPATCH11 Protein (rp375062) is not detailed in the source material provided. Researchers should refer to the official product datasheet available from Aladdin Scientific or contact customer support for confirmed expression host, tag information, and formulation details before planning experiments.

Can Recombinant Human GPATCH11 Protein be used as an ELISA standard or coating antigen?

Recombinant Human GPATCH11 Protein may be used as a coating antigen for ELISA-based antibody screening or as a standard for sandwich ELISA development, provided the protein retains appropriate epitope presentation under assay conditions. The suitability depends on protein purity, formulation buffer compatibility with the ELISA plate, and the specific antibodies used. Researchers are advised to pilot-test coating concentrations and blocking conditions before committing to large-scale assays.

References

1. Aladdin Scientific product page: Recombinant Human GPATCH11 Protein, SKU rp375062.
2. Sloan KE, Bohnsack MT. Unravelling the mechanisms of RNA helicase regulation. Trends Biochem Sci. 2018. [General reference for G-patch domain helicase regulation — verify applicability to GPATCH11]
3. Heininger AU, et al. Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. RNA Biol. 2016. [G-patch protein functional context — verify relevance to GPATCH11 specifically]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.