Recombinant Human GPBP1L1 Protein: Properties & Applications

Recombinant Human GPBP1L1 Protein is a research-grade recombinant protein designed to support investigations into GTPase-binding protein interactions, cytoskeletal organization, and downstream signal transduction pathways in human cellular systems.

Product Name

Recombinant Human GPBP1L1 Protein

SKU

rp367293

Species Origin

Human (Homo sapiens)

Protein Type

Recombinant protein (GTPase-binding protein family)

Primary Research Use

Protein interaction studies, cytoskeletal and signal transduction research

Supplier Reference

absea#PP-2788

What Is GPBP1L1? Gene and Protein Background

GPBP1L1 (GTPase-Binding Protein 1-Like 1) is a member of the GTPase-binding protein family, proteins characterized by their ability to interact with small GTPases and participate in the regulation of intracellular signaling cascades. The human GPBP1L1 gene encodes a protein that is implicated in cytoskeletal organization and cell morphology regulation, functions that are essential for normal cellular homeostasis.

GTPase-binding proteins serve as molecular scaffolds or effectors, linking activated GTPases such as members of the Rho and Ras superfamilies to downstream cytoskeletal and signaling components. Understanding the interactome and functional roles of GPBP1L1 is therefore relevant to multiple areas of cell biology, including cell migration, division, and differentiation.

Note: Detailed gene structure, isoform information, and tissue-expression data for GPBP1L1 should be verified against current entries in NCBI Gene and UniProt databases, as annotation may be updated.

Biochemical Properties of Recombinant Human GPBP1L1 Protein

The Recombinant Human GPBP1L1 Protein (rp367293) is produced using recombinant expression technology to ensure consistency, purity, and biological relevance for in vitro research applications. Recombinant production allows researchers to obtain defined quantities of the target protein free from contaminating human-source material.

Key biochemical considerations for recombinant GPBP1L1 include:

• Expression System: Specific host expression system (e.g., E. coli, baculovirus, HEK293) should be confirmed in the product datasheet.
• Purity: Research-grade recombinant proteins from Aladdin Scientific are typically produced to high purity; please consult the product specification sheet for lot-specific purity data.
• Formulation: Buffer composition, storage conditions, and recommended reconstitution procedures are detailed in the accompanying product documentation.
• Tag Information: The presence and type of affinity tag (e.g., His-tag, GST-tag) should be verified in the product datasheet (absea#PP-2788) to ensure compatibility with downstream assay formats.

Review flag: Specific molecular weight, purity percentage, endotoxin levels, and expression host are not confirmed in the source material and must be verified against the product datasheet before publication.

Research Applications of GPBP1L1 Recombinant Protein

Recombinant Human GPBP1L1 Protein is suitable for a range of in vitro biochemical and cell biology research applications. Typical uses for recombinant GTPase-binding proteins include:

• Protein–Protein Interaction Studies: Use as bait or prey in pull-down assays, co-immunoprecipitation (co-IP) experiments, or surface plasmon resonance (SPR) binding assays to map interaction partners of GPBP1L1.
• Enzyme and Binding Assays: Characterize the GTPase-binding affinity and specificity of GPBP1L1 toward Rho-family or other small GTPases.
• Antibody Validation: Serve as a positive control antigen for validating anti-GPBP1L1 antibodies in Western blot, ELISA, or immunofluorescence workflows.
• Structural Biology: Provide recombinant protein for crystallography, cryo-EM, or NMR studies aimed at resolving the three-dimensional structure of GPBP1L1.
• Cell-Free Functional Assays: Investigate the role of GPBP1L1 in reconstituted signaling or cytoskeletal assembly systems.

Researchers should validate the protein's activity and suitability for each specific application using appropriate controls as outlined in the product documentation.

Handling, Storage, and Quality Considerations

Proper handling and storage of recombinant proteins are critical to preserving activity and ensuring reproducible experimental results. The following general guidelines apply to recombinant proteins of this class; always defer to the specific instructions provided in the Aladdin Scientific product datasheet for rp367293:

• Storage Temperature: Recombinant proteins are generally stored at –20 °C or –80 °C to maintain stability. Avoid repeated freeze–thaw cycles by aliquoting upon first use.
• Reconstitution: If supplied lyophilized, reconstitute in the recommended buffer as specified in the datasheet. Allow the vial to equilibrate to room temperature before opening.
• Working Concentration: Determine the optimal working concentration empirically for each application. Refer to published literature or the datasheet for starting concentration recommendations.
• Sterility and Endotoxin: For cell-based assays, confirm endotoxin levels meet the requirements of your experimental system.
• Expiry and Lot Traceability: Record lot numbers and expiry dates for GLP/GMP compliance and experimental reproducibility.

Ordering Information and Support

Recombinant Human GPBP1L1 Protein is available from Aladdin Scientific under SKU rp367293 (supplier reference absea#PP-2788). To place an order or request a quotation, contact your local Aladdin Scientific representative or visit the Aladdin Scientific website.

For technical support regarding reconstitution, assay optimization, or application-specific guidance, Aladdin Scientific's scientific support team is available to assist. Custom quantities, bulk pricing, and special formulations may be available upon request.

When ordering, please ensure you specify the correct SKU and confirm the required quantity, format (liquid or lyophilized), and any specific quality documentation (e.g., Certificate of Analysis, SDS) required by your institution or regulatory framework.

Frequently asked questions

What is GPBP1L1 and why is it relevant to my research?

GPBP1L1 (GTPase-Binding Protein 1-Like 1) is a member of the GTPase-binding protein family involved in cytoskeletal organization and intracellular signaling. It is relevant to researchers studying Rho/Ras GTPase pathways, cell migration, morphology, or protein–protein interaction networks.

What applications is Recombinant Human GPBP1L1 Protein (rp367293) validated for?

This recombinant protein is suitable for protein–protein interaction studies (pull-down, co-IP, SPR), antibody validation (Western blot, ELISA), structural biology experiments, and cell-free functional assays. Researchers should validate activity for each specific application using appropriate controls. Please consult the product datasheet for confirmed validated applications.

How should I store Recombinant Human GPBP1L1 Protein to maintain stability?

In general, recombinant proteins should be stored at –20 °C or –80 °C and aliquoted to avoid repeated freeze–thaw cycles, which can reduce protein activity. Always follow the specific storage instructions provided in the Aladdin Scientific product datasheet for rp367293.

What is the supplier reference number for this product?

The supplier reference number for Recombinant Human GPBP1L1 Protein is absea#PP-2788. The Aladdin Scientific SKU is rp367293. Please use the Aladdin SKU when placing orders through Aladdin Scientific channels.

Can I request a Certificate of Analysis or Safety Data Sheet for rp367293?

Yes. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available for Recombinant Human GPBP1L1 Protein (rp367293). Please contact Aladdin Scientific customer service or your local representative to request these documents for your specific lot.

References

1. NCBI Gene Database. GPBP1L1 gene entry. National Center for Biotechnology Information.
2. UniProt Consortium. UniProtKB entry for human GPBP1L1 protein. Universal Protein Resource.
3. Aladdin Scientific. Product Datasheet: Recombinant Human GPBP1L1 Protein, SKU rp367293 (absea#PP-2788). Aladdin Scientific Inc.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.