Recombinant Human KRBA2 Protein: Research Guide & Applications

Recombinant Human KRBA2 Protein is a research-grade recombinant protein designed to support functional studies, interaction assays, and biochemical investigations of the KRBA2 gene product in human biology.

Product Name

Recombinant Human KRBA2 Protein

SKU

rp372961

Reference Catalog

absea#PP-6910

Protein Origin

Human (Homo sapiens)

Application

Biochemical assays, protein interaction studies, functional research

Storage Recommendation

Avoid repeated freeze-thaw cycles; store aliquots at -80 °C for long-term stability

Biological Background of KRBA2

KRBA2 (KRAB-A domain-containing protein 2) is a human protein encoded by the KRBA2 gene. It belongs to a family of proteins characterised by the presence of KRAB (Krüppel-associated box) domains, which are predominantly found in zinc finger transcription factors and are involved in transcriptional repression. KRBA2 has been associated with regulatory roles at the chromatin level, potentially influencing gene expression networks relevant to cellular differentiation and development.

Research into KRBA2 and related KRAB-domain proteins continues to expand, as this protein family represents one of the largest groups of transcriptional regulators in the mammalian genome. Understanding KRBA2 function may provide insights into epigenetic regulation and disease-associated transcriptional dysregulation.

Protein Characteristics and Product Specifications

Recombinant Human KRBA2 Protein (SKU: rp372961; reference: absea#PP-6910) is produced using recombinant expression systems to deliver a research-grade protein suitable for in vitro studies. Key characteristics to verify upon receipt include:

• Purity: Confirm purity grade from the product datasheet prior to use; typical recombinant proteins are supplied at >85% purity by SDS-PAGE unless otherwise stated.
• Formulation: Consult the product-specific datasheet for buffer composition, protein concentration, and excipients.
• Molecular Weight: Refer to the product datasheet for the confirmed molecular weight, as tags or truncations may alter the apparent mass on gel.
• Endotoxin Level: Review the certificate of analysis (CoA) for endotoxin content, particularly important for cell-based assays.

Note: Specific numerical values for purity, concentration, and molecular weight should be verified against the current product datasheet and CoA provided by Aladdin Scientific.

Handling, Reconstitution, and Storage Protocol

Proper handling of Recombinant Human KRBA2 Protein is essential to maintain biological activity and product integrity. Follow these general guidelines:

1. Upon Receipt: Inspect the vial immediately. If supplied lyophilised, store at -20 °C or -80 °C as directed. If supplied in liquid form, place on ice and process promptly.
2. Reconstitution: If lyophilised, reconstitute using the volume and buffer specified in the product datasheet (commonly sterile PBS or water). Allow the vial to equilibrate to room temperature before opening. Gently mix; do not vortex vigorously to avoid protein denaturation.
3. Aliquoting: Prepare single-use aliquots immediately after reconstitution to prevent activity loss from repeated freeze-thaw cycles.
4. Storage: Store aliquots at -80 °C for long-term storage. Short-term storage (up to 1 week) may be at -20 °C. Avoid temperatures above 4 °C for extended periods.
5. Working Concentration: Determine the optimal working concentration empirically for each assay type. Refer to published literature on KRAB-domain protein assays as a starting reference.

Always consult the product-specific datasheet for exact reconstitution volumes, recommended storage buffers, and stability data.

Research Applications

Recombinant Human KRBA2 Protein is intended for in vitro research use and may support a range of experimental applications, including:

• Protein–Protein Interaction Studies: Use in co-immunoprecipitation (Co-IP), pull-down assays, or surface plasmon resonance (SPR) to map interaction partners of KRBA2.
• Biochemical Characterisation: Enzyme-linked immunosorbent assays (ELISA), Western blot validation of antibodies, and dot blot analyses.
• Structural Studies: Suitable as a reference standard or antigen in structural biology workflows where recombinant protein is required.
• Functional Assays: Investigation of KRBA2 involvement in transcriptional regulatory complexes and chromatin-associated processes in cell-free systems.
• Immunogen Production: Can serve as an immunogen for the generation of custom antibodies targeting KRBA2.

This product is for research use only (RUO) and is not intended for diagnostic, therapeutic, or clinical use.

Quality Assurance and Troubleshooting

To ensure experimental reproducibility when working with Recombinant Human KRBA2 Protein, consider the following quality and troubleshooting guidance:

• Verify Identity on Receipt: Cross-reference the lot number and CoA with the packing slip. Confirm protein identity by SDS-PAGE or Western blot if required by your laboratory SOPs.
• Activity Loss: If reduced activity is observed, assess whether freeze-thaw cycles have been excessive, whether the protein was stored correctly, or whether the reconstitution buffer was appropriate.
• Aggregation: Protein aggregation may occur if stored at incorrect temperatures or at too high a concentration. Brief centrifugation (10,000 × g, 5 min, 4 °C) can pellet aggregates before use.
• Non-specific Binding in Assays: Include appropriate blocking steps and negative controls. Optimise salt and detergent concentrations in assay buffers to minimise background.
• Contacting Technical Support: For product-specific technical enquiries, contact Aladdin Scientific technical support, providing your lot number and CoA for efficient troubleshooting.

Frequently asked questions

What is Recombinant Human KRBA2 Protein used for in research?

Recombinant Human KRBA2 Protein (rp372961) is used for in vitro research applications including protein–protein interaction studies, biochemical characterisation assays such as ELISA and Western blot, structural biology, functional assays in cell-free systems, and as an immunogen for antibody generation. It is strictly for research use only and not for diagnostic or clinical purposes.

How should Recombinant Human KRBA2 Protein be stored to maintain activity?

For long-term storage, aliquot the protein immediately after reconstitution and store at -80 °C. Avoid repeated freeze-thaw cycles as these can significantly reduce protein activity. Short-term storage (up to one week) may be performed at -20 °C. Always refer to the product-specific datasheet for the recommended storage buffer and stability information.

How do I reconstitute lyophilised Recombinant Human KRBA2 Protein?

Allow the lyophilised vial to equilibrate to room temperature before opening. Add the reconstitution buffer (typically sterile PBS or purified water) as specified in the product datasheet. Gently swirl or pipette to mix; do not vortex vigorously. After reconstitution, prepare single-use aliquots and store at -80 °C to preserve protein integrity.

What quality documentation is available for Recombinant Human KRBA2 Protein (rp372961)?

Each lot of Recombinant Human KRBA2 Protein is accompanied by a Certificate of Analysis (CoA) detailing purity, concentration, endotoxin levels, and lot-specific test results. A product datasheet providing storage, reconstitution, and application guidance is also available. Contact Aladdin Scientific customer support or visit the product page to obtain these documents for your specific lot.

References

1. Aladdin Scientific Product Page: Recombinant Human KRBA2 Protein (rp372961). Aladdin Scientific.
2. absea Reference PP-6910: KRBA2 Protein Product Information. Absea Biotechnology.
3. Urrutia R. KRAB-containing zinc-finger repressor proteins. Genome Biology. 2003;4(10):231. doi:10.1186/gb-2003-4-10-231

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.