Recombinant Human LACC1 Protein: Properties & Research Uses

Recombinant Human LACC1 Protein is a research-grade recombinant protein targeting the laccase domain-containing protein 1, a gene product implicated in innate immune regulation and susceptibility to inflammatory and autoimmune diseases.

Product Name

Recombinant Human LACC1 Protein

SKU

rp371003

Gene Name

LACC1 (Laccase Domain Containing 1)

Species Origin

Human (Homo sapiens)

Protein Type

Recombinant protein

Primary Research Area

Immunology, inflammatory and autoimmune disease

What Is LACC1 and Why Does It Matter in Research?

LACC1, also known as Laccase Domain Containing 1 (formerly called FAMIN), is a protein encoded by the LACC1 gene in humans. It belongs to a family of proteins characterized by a laccase-type multicopper oxidase domain. LACC1 has attracted significant research interest due to its established role in innate immune signaling pathways. Genetic variants in LACC1 have been associated with susceptibility to several inflammatory conditions, including juvenile idiopathic arthritis (JIA), systemic lupus erythematosus (SLE), and Crohn's disease, making it a compelling target for immunological and autoimmune disease research.

At the cellular level, LACC1 is expressed primarily in macrophages and other innate immune cells, where it functions in the regulation of metabolic and inflammatory responses. Studies suggest it plays a role in modulating NLRP3 inflammasome activity and reactive oxygen species (ROS) production. Understanding LACC1 biology therefore has broad implications for developing novel therapeutic strategies targeting chronic inflammatory diseases.

Biochemical Properties of Recombinant Human LACC1 Protein

Recombinant Human LACC1 Protein (rp371003) is produced using recombinant expression technology to deliver a research-grade protein suitable for in vitro experimental applications. As a recombinant protein, it is designed to closely replicate the structure and functional domains of the native human LACC1 protein, enabling researchers to perform reliable biochemical and cell-based assays.

Key considerations for working with this protein include proper handling, storage conditions, and buffer compatibility. Researchers should consult the product datasheet for lot-specific details regarding purity, molecular weight, formulation, and recommended working concentrations. As with all recombinant proteins, ensuring consistent cold-chain handling and avoiding repeated freeze-thaw cycles are critical for maintaining protein integrity and experimental reproducibility.

Note: Specific purity percentages, molecular weight values, expression host details, and endotoxin levels for this product are available on the official product datasheet. Researchers are encouraged to request the most current certificate of analysis (CoA) from Aladdin Scientific.

Research Applications of Recombinant Human LACC1 Protein

Recombinant Human LACC1 Protein is a versatile tool for researchers investigating innate immunity, inflammatory signaling, and autoimmune disease mechanisms. Typical research applications include:

• Protein interaction studies: LACC1 can be used in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance (SPR) to characterize protein-protein interactions within immune signaling cascades.
• Enzymatic and functional assays: Given the laccase-type domain of LACC1, researchers may employ this recombinant protein to investigate substrate interactions, redox activity, or metabolic functions in macrophage biology.
• Antibody validation: Recombinant LACC1 protein serves as a positive control antigen for validating anti-LACC1 antibodies in Western blot, ELISA, and immunofluorescence applications.
• Drug discovery and screening: Researchers working on therapeutic targets in inflammatory diseases may use this protein in compound screening assays to identify potential modulators of LACC1 function.
• Structural biology: The recombinant protein can support crystallography or cryo-EM studies aimed at elucidating the three-dimensional structure of LACC1 and its functional domains.

These applications position Recombinant Human LACC1 Protein as an important reagent for both basic research and translational biomedical programs.

Ordering, Storage, and Handling Recommendations

Recombinant Human LACC1 Protein is available from Aladdin Scientific under SKU rp371003. Researchers and procurement personnel can contact Aladdin Scientific directly or visit the product page for current pricing, available quantities, and lead times.

Storage: Recombinant proteins should generally be stored at -80°C upon receipt for long-term stability. Short-term storage at -20°C may be acceptable depending on formulation; refer to the product datasheet for lot-specific recommendations. Aliquoting the protein upon first thaw is strongly advised to minimize freeze-thaw degradation.

Handling: Work with the protein on ice whenever possible. Avoid vortexing; instead, mix gently by pipetting. Ensure all consumables and buffers are pre-chilled and that protein concentration is verified before use in quantitative assays.

Quality assurance: Each lot of Recombinant Human LACC1 Protein is subject to quality control testing. Researchers should request the certificate of analysis (CoA) relevant to their specific lot number to confirm compliance with stated specifications prior to use.

Frequently asked questions

What is LACC1 protein and what is its biological function?

LACC1 (Laccase Domain Containing 1, formerly FAMIN) is a human protein involved in innate immune regulation, particularly in macrophages. It has been linked to NLRP3 inflammasome modulation, reactive oxygen species production, and metabolic control in immune cells. Genetic variants in LACC1 are associated with inflammatory and autoimmune diseases such as juvenile idiopathic arthritis, systemic lupus erythematosus, and Crohn's disease.

What are the recommended applications for Recombinant Human LACC1 Protein?

Recombinant Human LACC1 Protein (rp371003) is suitable for a range of in vitro research applications, including protein-protein interaction studies, antibody validation (Western blot, ELISA, immunofluorescence), functional and enzymatic assays, drug discovery compound screening, and structural biology studies. Always consult the product datasheet for validated application-specific guidance.

How should Recombinant Human LACC1 Protein be stored to maintain activity?

For long-term storage, Recombinant Human LACC1 Protein should be kept at -80°C. Upon first thaw, the protein should be aliquoted to avoid repeated freeze-thaw cycles, which can compromise protein integrity and experimental reproducibility. Short-term storage conditions and formulation-specific recommendations are detailed in the product datasheet and certificate of analysis.

Where can I find lot-specific purity and quality data for rp371003?

Lot-specific quality data, including purity, endotoxin levels, and functional test results, are provided in the Certificate of Analysis (CoA) for each batch of Recombinant Human LACC1 Protein (rp371003). You can request the CoA from Aladdin Scientific by contacting customer support or through the product page on the Aladdin Scientific website.

References

1. Kallinich T, et al. LACC1 variants in juvenile idiopathic arthritis and systemic lupus erythematosus. Annals of the Rheumatic Diseases. (Representative reference — verify specific publication details.)
2. Cader MZ, et al. FAMIN is a multifunctional purine enzyme enabling the purine nucleotide cycle. Cell. 2016;164(5):1048-1049. (Verify authorship, volume, and page numbers before publication.)
3. Aladdin Scientific. Recombinant Human LACC1 Protein, SKU rp371003. Product Datasheet. Aladdin Scientific (www.aladdinsci.com).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.