Recombinant Human LRRN4 Protein: Properties & Research Uses

Recombinant Human LRRN4 Protein is a leucine-rich repeat neuronal protein produced recombinantly for use as a research reagent in neuroscience, cell biology, and protein–protein interaction studies.

Protein Name

Leucine-Rich Repeat Neuronal Protein 4 (LRRN4)

SKU

rp369293

Protein Family

Leucine-Rich Repeat (LRR) superfamily

Species Origin

Human (Homo sapiens)

Primary Research Areas

Neuroscience, cell adhesion, protein–protein interactions

Format

Recombinant protein (details: see product datasheet)

What Is LRRN4? Gene and Protein Background

LRRN4 (Leucine-Rich Repeat Neuronal Protein 4) is a member of the leucine-rich repeat (LRR) superfamily, a large and functionally diverse group of proteins characterized by tandem LRR motifs that mediate protein–protein interactions. The LRRN4 gene encodes a type I transmembrane protein that is predominantly expressed in neural tissues, where it is implicated in cell adhesion, neurite outgrowth, and the organization of neuronal circuits.

LRR-containing proteins are evolutionarily conserved and play critical roles in developmental signaling, immune recognition, and synaptic function. LRRN4 specifically has been associated with processes relevant to neural development and connectivity, making it a subject of interest in both basic neuroscience and translational research contexts.

Recombinant Human LRRN4 Protein: Production and Biochemical Properties

Recombinant Human LRRN4 Protein (SKU: rp369293) is produced using recombinant expression systems to deliver a research-grade protein suitable for in vitro experimentation. Recombinant production ensures batch-to-batch consistency, defined purity, and the absence of native biological contaminants that may complicate experimental interpretation.

Key biochemical properties of recombinant LRR-family proteins such as LRRN4 typically include a defined molecular weight reflective of the encoded polypeptide and any post-translational modifications introduced by the expression system, as well as a characteristic LRR domain architecture that confers binding functionality. Researchers should consult the official product datasheet for lot-specific details including purity grade, formulation buffer, recommended storage conditions, and endotoxin levels.

Note: Specific numerical values for purity, molecular weight, and endotoxin content are lot-dependent. Always refer to the Certificate of Analysis (CoA) supplied with each shipment.

Research Applications of Recombinant Human LRRN4 Protein

Recombinant Human LRRN4 Protein is a versatile reagent applicable across several experimental workflows in neuroscience and molecular cell biology:

• Protein–Protein Interaction Studies: The LRR domain of LRRN4 is well-suited for binding assays such as co-immunoprecipitation, pull-down experiments, and surface plasmon resonance (SPR), enabling identification and characterization of LRRN4 binding partners.
• Cell Adhesion and Neurite Outgrowth Assays: As a neuronal adhesion-related protein, recombinant LRRN4 can be used in substrate-coating experiments to investigate its role in promoting or modulating neurite extension and cell attachment.
• ELISA and Immunoassay Development: High-purity recombinant LRRN4 protein serves as a standard or coating antigen in the development and validation of immunoassays targeting this protein.
• Antibody Validation: Recombinant protein is a gold-standard positive control for validating anti-LRRN4 antibodies in Western blot, ELISA, and immunofluorescence applications.
• Structural and Biophysical Studies: The defined recombinant format supports crystallography, cryo-EM sample preparation, and other structural biology approaches aimed at elucidating the three-dimensional architecture of LRRN4.

Handling, Storage, and Reconstitution Guidelines

Proper handling and storage of recombinant proteins are essential to maintaining activity and integrity over time. The following general guidelines apply to Recombinant Human LRRN4 Protein; always consult the product-specific datasheet and CoA for definitive instructions:

• Storage Temperature: Recombinant proteins are typically stored at −20 °C or −80 °C to preserve stability. Avoid repeated freeze–thaw cycles, which can lead to aggregation and loss of activity.
• Reconstitution: If supplied in lyophilized form, reconstitute with sterile, ultrapure water or the buffer specified in the datasheet. Allow the vial to equilibrate to room temperature before opening.
• Aliquoting: Upon reconstitution, prepare single-use aliquots to minimize freeze–thaw cycles and store at the recommended temperature.
• Carrier Protein: Addition of a carrier protein such as BSA (0.1–0.5%) may enhance stability during long-term storage at the discretion of the researcher and per datasheet guidance.
• Working Concentration: Determine empirically for each application; refer to relevant literature or the datasheet for suggested starting concentrations.

For lot-specific formulation details, endotoxin values, and stability data, refer to the Certificate of Analysis included with your shipment or contact Aladdin Scientific technical support.

Ordering Information and Technical Support

Recombinant Human LRRN4 Protein is available from Aladdin Scientific under SKU rp369293. To order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding experimental design, recommended assay conditions, or protein handling, Aladdin Scientific's technical support team is available to assist. When contacting support, please have your lot number and CoA on hand to facilitate efficient troubleshooting.

Bulk quantities and custom formulations may be available upon request; inquire with the sales team for details regarding minimum order quantities and lead times.

Frequently asked questions

What is Recombinant Human LRRN4 Protein used for in research?

Recombinant Human LRRN4 Protein is used in a range of applications including protein–protein interaction studies, cell adhesion and neurite outgrowth assays, ELISA and immunoassay development, antibody validation, and structural biology experiments. Its LRR domain makes it particularly useful for binding and functional characterization studies in neuroscience research.

How should Recombinant Human LRRN4 Protein be stored to maintain stability?

This protein should generally be stored at −20 °C or −80 °C, depending on lot-specific recommendations provided in the datasheet and Certificate of Analysis. Repeated freeze–thaw cycles should be avoided by preparing single-use aliquots upon reconstitution. Always refer to the product-specific CoA for definitive storage guidance.

Is Recombinant Human LRRN4 Protein suitable for use as a Western blot positive control?

Yes, high-purity recombinant human LRRN4 protein is an excellent positive control for validating anti-LRRN4 antibodies in Western blot applications. It provides a defined, consistent band at the expected molecular weight, facilitating reliable antibody and assay validation. Refer to the product datasheet for guidance on recommended loading amounts.

Where can I find lot-specific purity and endotoxin data for rp369293?

Lot-specific data including purity, endotoxin levels, molecular weight, and formulation details are provided in the Certificate of Analysis (CoA) shipped with each order. You may also contact Aladdin Scientific technical support or visit the product page on the Aladdin Scientific website to request a CoA for a specific lot number.

References

1. UniProt Consortium. UniProt entry for human LRRN4 (Leucine-rich repeat neuronal protein 4). UniProt Knowledgebase.
2. Bella J, Hindle KL, McEwan PA, Lovell SC. The leucine-rich repeat structure. Cell Mol Life Sci. 2008;65(15):2307-2333. doi:10.1007/s00018-008-8019-0

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.