Recombinant Human MCF2L2 Protein: FAQs & Key Facts

Recombinant Human MCF2L2 Protein is a research-grade recombinant protein corresponding to the human MCF2L2 gene product, a Dbl-family guanine nucleotide exchange factor (GEF) involved in Rho GTPase signaling pathways, and is available from Aladdin Scientific (SKU: rp375347) for use in biochemical and cell biology research applications.

Product Name

Recombinant Human MCF2L2 Protein

SKU

rp375347

Protein Family

Dbl-family Guanine Nucleotide Exchange Factor (GEF)

Species Origin

Human (Homo sapiens)

Primary Function

Activation of Rho-family GTPases via GDP-to-GTP exchange

Recommended Storage

-80°C for long-term storage; avoid repeated freeze-thaw cycles

Supplier Reference

absea #PP-9046

What Is MCF2L2 and Why Is It Relevant to Research?

MCF2L2 (MCF.2 cell line derived transforming sequence-like 2) is a member of the Dbl family of guanine nucleotide exchange factors (GEFs). GEFs of this family catalyze the exchange of GDP for GTP on Rho-family small GTPases, thereby activating downstream effectors involved in cytoskeletal remodeling, cell migration, and proliferation. MCF2L2 specifically has been associated with the regulation of RhoA and Rac1 signaling axes, making it a relevant target in studies of oncogenesis, neuronal development, and cell polarity.

Researchers studying GTPase signal transduction, cancer biology, or cytoskeletal dynamics will find Recombinant Human MCF2L2 Protein a useful tool for in vitro GEF activity assays, protein-protein interaction studies, and antibody validation experiments.

Product Specifications and Quality

Recombinant Human MCF2L2 Protein (SKU: rp375347) is supplied by Aladdin Scientific with reference to absea catalog number PP-9046. As with all Aladdin recombinant proteins, this product is manufactured under controlled conditions designed to deliver consistent purity and biological activity suitable for research use.

Key specification areas to confirm with the product datasheet:

• Expression system: Confirm host system (e.g., E. coli, baculovirus, mammalian HEK293) on the official datasheet, as this affects post-translational modification status.
• Purity: Typically >85% or >95% as determined by SDS-PAGE; verify exact figure on the datasheet.
• Tag information: May include His-tag, GST-tag, or be tag-free; confirm on current datasheet.
• Formulation: Supplied lyophilized or in liquid buffer; refer to the Certificate of Analysis (CoA) for the specific lot.

Note: Specific numerical values for purity, molecular weight, and endotoxin levels should be verified against the current product datasheet and CoA, as these may vary by lot.

Storage, Reconstitution, and Handling

Proper handling of Recombinant Human MCF2L2 Protein is essential to maintaining its activity and integrity. The following general best practices apply; always cross-reference with the product-specific datasheet.

Storage: Store at -80°C upon receipt for long-term preservation. Short-term storage at -20°C may be acceptable for periods specified on the datasheet. Avoid repeated freeze-thaw cycles, as these can cause protein aggregation and loss of activity. Consider preparing single-use aliquots immediately after reconstitution.

Reconstitution (lyophilized format): Briefly centrifuge the vial before opening. Reconstitute in the buffer recommended on the datasheet (typically sterile water, PBS, or a Tris-based buffer) to the suggested concentration. Allow the solution to equilibrate at room temperature for a few minutes and mix gently; do not vortex. Carrier protein (e.g., 0.1% BSA) may be added to stabilize dilute solutions.

Working concentrations: Optimize empirically for each assay type. Refer to the datasheet for suggested starting concentrations.

Applications and Compatibility

Recombinant Human MCF2L2 Protein is intended for research use only and is not approved for diagnostic or therapeutic applications. Typical research applications include:

• In vitro GEF activity assays: Measuring the rate of GDP-to-GTP exchange on Rho-family GTPase substrates.
• Protein-protein interaction studies: Pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance (SPR) binding studies.
• Antibody validation: As a positive control antigen in Western blot or ELISA for anti-MCF2L2 antibodies.
• Structural studies: Crystallography or cryo-EM sample preparation (purity and buffer compatibility should be confirmed).
• Cell-free biochemical assays: Investigating enzyme kinetics or inhibitor screening.

Compatibility with specific downstream assay reagents should be validated in the user's own laboratory conditions. Aladdin Scientific technical support can assist with application-specific guidance.

Ordering, Shipping, and Returns

Recombinant Human MCF2L2 Protein (SKU: rp375347) can be ordered directly through the Aladdin Scientific website or via your institutional procurement channel. The following information is relevant for procurement and lab managers:

Ordering: Reference SKU rp375347 when placing an order. Bulk or custom quantities may be available; contact Aladdin Scientific sales for quotations.

Shipping: Recombinant proteins are temperature-sensitive. Aladdin Scientific ships cold-chain products on dry ice to maintain integrity during transit. Please inspect the package upon receipt and note any signs of compromised cold chain in writing before accepting.

Returns and quality issues: If the product does not meet the specifications stated on the datasheet or CoA, contact Aladdin Scientific customer service promptly. Retain the original packaging and shipping materials. Returns and refunds are subject to Aladdin Scientific's standard return policy; please review terms on the website or contact support for guidance.

Payment and pricing: Pricing may vary by region and quantity. Academic and institutional discounts may apply. Contact the Aladdin Scientific sales team or check the website for current promotions.

Frequently asked questions

What is the function of MCF2L2 protein in human cells?

MCF2L2 is a Dbl-family guanine nucleotide exchange factor (GEF) that activates Rho-family small GTPases, including RhoA and Rac1, by catalyzing the exchange of GDP for GTP. This activity regulates downstream pathways involved in cytoskeletal remodeling, cell migration, and proliferation. Its dysregulation has been linked to oncogenesis and developmental processes.

What expression system is used to produce Recombinant Human MCF2L2 Protein (rp375347)?

The specific expression system (e.g., E. coli, baculovirus-insect cell, or mammalian HEK293) for SKU rp375347 should be confirmed on the current product datasheet or Certificate of Analysis available from Aladdin Scientific. The choice of expression system affects post-translational modifications and biological activity, so verifying this detail is important for assay design.

How should Recombinant Human MCF2L2 Protein be stored and handled to maintain activity?

For long-term storage, keep the protein at -80°C. Avoid repeated freeze-thaw cycles by preparing single-use aliquots after initial reconstitution or thawing. If the product is supplied lyophilized, reconstitute in the buffer specified on the datasheet and mix gently without vortexing. Adding a carrier protein such as 0.1% BSA can help stabilize dilute working solutions.

What are the recommended research applications for Recombinant Human MCF2L2 Protein?

Recombinant Human MCF2L2 Protein is suitable for in vitro GEF activity assays, protein-protein interaction studies (such as pull-down or SPR), antibody validation as a positive control antigen in Western blot or ELISA, and cell-free biochemical assays including inhibitor screening. It is for research use only and is not intended for diagnostic or therapeutic applications.

How do I place an order for Recombinant Human MCF2L2 Protein and what are the shipping conditions?

You can order Recombinant Human MCF2L2 Protein (SKU: rp375347) through the Aladdin Scientific website or via institutional procurement channels by referencing the SKU. The product is shipped on dry ice to maintain cold-chain integrity. Upon receipt, inspect the package for any cold-chain compromise and contact Aladdin Scientific customer service immediately if any issues are identified.

References

1. Rossman KL, Der CJ, Sondek J. GEF means go: turning on RhoGTPases with guanine nucleotide-exchange factors. Nature Reviews Molecular Cell Biology. 2005;6(2):167-180. doi:10.1038/nrm1587
2. Bhavna absea product reference PP-9046, Recombinant Human MCF2L2 Protein datasheet. Absea Biotechnology.
3. Etienne-Manneville S, Hall A. Rho GTPases in cell biology. Nature. 2002;420(6916):629-635. doi:10.1038/nature01148

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.