Recombinant Human MED15 Protein: Properties & Usage Guide

Recombinant Human MED15 Protein is a research-grade recombinant subunit of the Mediator transcriptional coactivator complex, produced to support mechanistic studies of RNA polymerase II-dependent gene regulation and related signal transduction pathways.

Product Name

Recombinant Human MED15 Protein

SKU

rp372097

Protein Origin

Human (Homo sapiens)

Protein Class

Mediator complex subunit; transcriptional coactivator

Primary Application

Transcription regulation research, protein-protein interaction studies, Mediator complex assembly assays

Supplier

Aladdin Scientific

Background: MED15 and the Mediator Complex

MED15 (Mediator of RNA Polymerase II Transcription Subunit 15) is an integral component of the Mediator complex, a highly conserved multi-subunit assembly that acts as a molecular bridge between gene-specific transcription factors and the RNA polymerase II (Pol II) general transcription machinery. The Mediator complex is essential for the regulated transcription of virtually all protein-coding genes in eukaryotes.

MED15 resides in the tail module of the Mediator complex and plays a pivotal role in signal-dependent transcriptional activation. It has been shown to interact with a variety of transcriptional activators, including nuclear receptors and other sequence-specific transcription factors, thereby facilitating the transmission of regulatory signals to the core transcription apparatus. MED15 is also known by alternative designations including PCQAP and ARC105 in earlier literature.

Given its central role in transcriptional control, MED15 is of broad interest in studies of development, differentiation, metabolic regulation, and disease-associated transcriptional reprogramming.

Product Description and Key Specifications

Aladdin Scientific's Recombinant Human MED15 Protein (SKU: rp372097) is produced using recombinant expression technology to deliver a research-grade protein suitable for in vitro biochemical and cell-free assays. The product is intended exclusively for research use and is not approved for diagnostic or therapeutic applications.

Key product attributes to verify with the datasheet (contact Aladdin Scientific for the current Certificate of Analysis) include:

• Expression System: Confirm host system (e.g., E. coli, baculovirus/insect cell, or mammalian) from the product datasheet, as this affects post-translational modification status.
• Purity: Research-grade recombinant proteins from Aladdin Scientific are typically evaluated by SDS-PAGE; confirm target purity percentage from the CoA.
• Formulation & Buffer: Review the supplied buffer composition and any carrier protein or stabilizer (e.g., BSA, glycerol) from the product datasheet prior to use.
• Molecular Weight: Confirm the expected apparent molecular weight on SDS-PAGE from the product datasheet, noting that tags or truncations may alter migration.

Note: Specific numerical values for purity, concentration, and molecular weight are not reproduced here; always refer to the current product datasheet and Certificate of Analysis (CoA) provided by Aladdin Scientific.

Recommended Applications

Recombinant Human MED15 Protein is designed to support a range of in vitro research applications related to transcription biology and protein biochemistry. Typical research uses include, but are not limited to:

• Protein–Protein Interaction Studies: MED15 can be used in pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance (SPR) experiments to characterize its interactions with transcription factors, nuclear receptors, and other Mediator subunits.
• Structural and Biophysical Studies: The recombinant protein may serve as a component in structural biology workflows, including X-ray crystallography or cryo-EM studies of Mediator submodule assemblies.
• In Vitro Transcription Assays: MED15 may be incorporated into cell-free transcription reconstitution systems to assess its functional contribution to Mediator-dependent gene activation.
• Antibody Validation: The purified recombinant protein is suitable as a positive control antigen for validating anti-MED15 antibodies in Western blot, ELISA, or dot-blot formats.
• Biochemical Screening: MED15 can serve as a target protein in small-molecule or peptide screening assays aimed at modulating transcription factor–coactivator interactions.

Researchers should validate the protein for each specific assay format under their own experimental conditions.

Storage, Handling, and Stability Guidelines

Proper handling of Recombinant Human MED15 Protein is essential to preserve biological activity and ensure reproducible experimental results. Follow these general best-practice guidelines, and always defer to the specific instructions provided in the product datasheet:

• Upon Receipt: Inspect the shipment immediately. If the protein is supplied lyophilized, store at –20 °C or –80 °C as indicated. If supplied in liquid form, store at the temperature specified on the datasheet (typically –80 °C for long-term storage).
• Avoid Freeze–Thaw Cycles: Repeated freeze–thaw cycling can lead to protein aggregation and loss of activity. Aliquot the protein into single-use volumes upon first thaw to minimize degradation.
• Reconstitution (Lyophilized Form): Reconstitute using the buffer and volume specified on the product datasheet. Allow the vial to equilibrate to room temperature briefly before reconstitution, and mix gently—do not vortex.
• Working Conditions: Keep the protein on ice during experimental setup. For extended bench-top use, some proteins may tolerate 4 °C for short periods; confirm stability data from the CoA.
• Contamination Prevention: Use sterile, low-protein-binding tubes and pipette tips. Avoid introducing nucleases or proteases into the protein solution.

Specific storage temperature, shelf life, and buffer composition are detailed on the product datasheet and CoA. Contact Aladdin Scientific technical support for clarification.

Quality Control and Ordering Information

Aladdin Scientific is committed to supplying research reagents that meet rigorous quality standards. The Recombinant Human MED15 Protein (rp372097) is subject to quality control testing prior to release. Typical QC assessments for recombinant proteins in the Aladdin portfolio may include:

• Purity Analysis: SDS-PAGE under reducing and/or non-reducing conditions to confirm protein integrity and purity.
• Identity Confirmation: Western blot or mass spectrometry confirmation of target protein identity.
• Endotoxin Testing: LAL (Limulus Amebocyte Lysate) assay to verify endotoxin levels where relevant to the application.
• Activity Assessment: Functional or binding activity data where applicable; confirm availability from the product datasheet.

To order Recombinant Human MED15 Protein, use SKU rp372097 on the Aladdin Scientific website or contact your regional Aladdin representative. Bulk quantities, custom formulations, or specific activity requirements may be accommodated upon request—contact technical support for details.

All QC data are lot-specific; request the current Certificate of Analysis (CoA) when placing your order or upon receipt of the product.

Frequently asked questions

What is MED15 and why is it important for transcription research?

MED15 is a subunit of the Mediator complex, a conserved multi-protein assembly that bridges gene-specific transcription factors and the RNA polymerase II machinery. Located in the tail module of Mediator, MED15 facilitates signal-dependent transcriptional activation by interacting with nuclear receptors and other activators, making it a key target in studies of gene regulation, development, and disease-associated transcriptional changes.

What applications is Recombinant Human MED15 Protein (rp372097) suitable for?

The protein is suitable for a range of in vitro research applications including protein–protein interaction assays (pull-down, SPR), antibody validation (Western blot, ELISA), in vitro transcription reconstitution, structural biology studies, and biochemical screening of small molecules targeting the Mediator–transcription factor interface. Researchers should validate the product for each specific application and assay format.

How should I store Recombinant Human MED15 Protein to maintain its activity?

Upon receipt, store the protein according to the temperature specified on the product datasheet—typically –80 °C for long-term storage of liquid formulations or lyophilized preparations. To avoid activity loss, aliquot into single-use volumes to prevent repeated freeze–thaw cycles. Always consult the specific CoA and datasheet for lot-specific storage conditions.

Can I use Recombinant Human MED15 Protein for diagnostic or therapeutic purposes?

No. Recombinant Human MED15 Protein (rp372097) from Aladdin Scientific is intended strictly for in vitro research use only. It is not approved, validated, or intended for use in diagnostic procedures, therapeutic applications, or any in vivo studies in humans or animals.

How do I request a Certificate of Analysis (CoA) for rp372097?

You can request the lot-specific Certificate of Analysis for Recombinant Human MED15 Protein (rp372097) by contacting Aladdin Scientific customer support or your regional sales representative, providing your lot number found on the product vial label. The CoA contains critical quality data including purity, identity, endotoxin level, and storage recommendations.

References

1. Conaway RC, Conaway JW. Function and regulation of the Mediator complex. Current Opinion in Genetics & Development. 2011;21(2):225-230. doi:10.1016/j.gde.2011.01.013
2. Malik S, Roeder RG. The metazoan Mediator co-activator complex as an integrative hub for transcriptional regulation. Nature Reviews Genetics. 2010;11(11):761-772. doi:10.1038/nrg2901
3. Yang F, et al. An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis. Nature. 2006;442(7103):700-704. doi:10.1038/nature04942
4. Aladdin Scientific. Recombinant Human MED15 Protein, SKU rp372097. Product Datasheet. Aladdin Scientific; [current version].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.