In protein research and pathology, WB, FC, IP/ChIP, IF, and IHC all rely heavily on antibodies. The same target often requires application-specific antibodies because each technique differs in antigen conformation, sample processing (denaturation/fixation/crosslinking), detection context (cell ...
Topic
Antibodies
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
14 resources · protocols, FAQs & articles
How to choose positive controls for antibodies?
What is the concentration of the primary antibody?
First, we should harvest our cells or tissue and prepare a single-cell suspension. We will then transfer our single-cell suspension into 96-well plates, test tubes, or polystyrene round bottom tubes, depending on the number of cells and volumes being used.
Will Aladdin antibodies recognize target protein in complex biological fluids such as blood or serum?
Can you tell me what epitope your antibody binds to?
Are Aladdin’s antibodies suitable for use in ELISA and Western Blot applications?
What information should be known about the stability of your antibody products?
How are your antibodies purified?
Have Aladdin’s antibodies been tested in neutralization assays?
Which isotype are Aladdin’s polyclonal antibodies?
Will Aladdin antibodies work in immunohistochemistry and immunocytochemistry applications?
Do Aladdin’s antibody products contain any carrier proteins or other additives?
Will Aladdin antibodies recognize target proteins sold by other vendors?
Related topics
Techniques often explored alongside immunological experiments.