Terrific Broth, Complete with Carbon Source (Powder)

Directions per Liter:

Dissolve 51.6 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). If precipitate forms after autoclaving, medium may be filter-sterilized using a 0.2 micron filter.

Component	g/liter
Potassium phosphate dibasic	9.4
Potassium phosphate monobasic	2.2
Sodium Pyruvate	4
Tryptone	12
Yeast Extract	24
	Total: 51.6g/Liter
	500g makes 9.7 liters

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.

Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.

Homogeneous: Proprietary blending technology ensures complete mixing of components.