RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) - BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥95%(HPLC) , CAS No.2711762-70-6

CAS: 2711762-70-6 Cat. No.: F1455371

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥95%(HPLC)

Synonyms

Fluorescent Probe for Intracellular L Fe²⁺ Fluorescent Probe bile Fe²⁺ | Intracellular Labile Fe²⁺ Fluorescent Probe | Fe2+ indicator

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

 Iron is the most abundant transition metal element in organisms and participates in a wide range of physiological processes. In recent years, labile intracellular iron has attracted extensive research attention. Labile iron primarily exists in two stable redox states: ferrous ion (Fe²⁺) and ferric ion (Fe³⁺). For researchers investigating the intracellular reductive environment, metal transporters and the aqueous solubility of Fe²⁺, characterizing the behavior of Fe²⁺ is more critical than studying Fe³⁺. As a novel fluorescent probe, RhoNox-4 enables fluorescence imaging of intracellular Fe²⁺ in live cells. The fluorescence intensity enhancement induced by the reaction between RhoNox-4 and Fe²⁺ is irreversible, which distinguishes it from calcium-sensing fluorescent probes such as Fluo-3 (Cat.No.: F131394) that support real-time monitoring of calcium ions.

Specifications

Synonyms

Fluorescent Probe for Intracellular L Fe²⁺ Fluorescent Probe bile Fe²⁺ | Intracellular Labile Fe²⁺ Fluorescent Probe | Fe2+ indicator

Specifications & Purity

BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥95%(HPLC)

Stability And Storage

Store at -20℃ long term (12 months). Store in the dark.

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Biological Stain, BioReagent, for Fluorescence analysis, for Microscopy

Purity

≥95%(HPLC)

Images

IF: RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) (F1455371)

RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) (F1455371) - Flow 

Flow cytometric histograms of Jurkat cells stained with FerroOrange. 

Black: untreated cells without probe (negative control); 

Blue: untreated cells with probe (basal Fe²⁺ level); 

Red: cells pretreated with 100 μM ferrous ammonium sulfate (FAS) (A112646) for 30 min before probe incubation (elevated Fe²⁺); 

Green: cells pretreated with 100 μM 2,2'-bipyridyl (BPY) (D108977) for 30 min before probe incubation (Fe²⁺ chelation).

Flow: RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) (F1455371)

RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) (F1455371) - IF 

Confocal fluorescence images of Jurkat cells stained with the Fe²⁺ probe FerroOrange (RhoNox-4). 

Left: untreated cells show weak red fluorescence, indicating endogenous Fe²⁺. 

Middle: after treatment with 100 μM ferrous ammonium sulfate (FAS) (A112646) for 30 min, the red fluorescence intensity increases significantly, reflecting elevated intracellular Fe²⁺. 

Right: addition of 100 μM 2,2'-bipyridyl (BPY) (D108977), a Fe²⁺ chelator, binds Fe²⁺ and prevents its reaction with FerroOrange, resulting in a marked decrease in red signal.

Chemical and Physical Properties

• Solubility: Soluble in acetonitrile, methanol and dimethyl sulfoxide (DMSO).