GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Host species
Mouse
Specificity
FLI1
Immunogen
A synthetic peptide derived from human FLI1
Positive Control
WB: Jurkat, THP-1 and Ramos cell lysates. IHC: Human hemangioendothelioma and pnet tissues.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Mouse IgG1
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
FLI1 Mouse mAb (Ab103739) - Western Blot All lanes: FLI1 Mouse mAb (Ab103739) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 51 kDa Observed band size: 48, 52 kDa FLI1 Mouse mAb (Ab103739) recognizes different isoforms of FLI1. Exposure time: 60.0 s
FLI1 Mouse mAb (Ab103739) - Western Blot All lanes: FLI1 Mouse mAb (Ab103739) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 51 kDa Observed band size: 48, 51 kDa FLI1 Mouse mAb (Ab103739) recognizes different isoforms of FLI1. Exposure time: 100.0 s
FLI1 Mouse mAb (Ab103739) - IHC Immunohistochemical analysis of FLI1 staining in human hemangioendothelioma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FLI1 Mouse mAb (Ab103739) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
FLI1 Mouse mAb (Ab103739) - IHC Immunohistochemical analysis of FLI1 staining in human pnet formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FLI1 Mouse mAb (Ab103739) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/100-1/300
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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