10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 32 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
Formononetin (Formononetol, Biochanin B) is a phytoestrogen from the root of Astragalus membranaceus and an O-methylated isoflavone.
In vitro
In vivo
Cell Data
cell lines：
Concentrations：
Incubation Time：
Powder Purity:≥99%
| Isomeri SMILES | COC1=CC=C(C=C1)C2=COC3=C(C2=O)C=CC(=C3)O |
|---|---|
| WGK Germania | 3 |
| RTECS | DJ3100114 |
| Peso molecolare | 268.26 |
| Beilstein | 237979 |
| Reaxy-Rn | 237979 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=237979&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Data | Oggetto |
|---|---|---|---|
 F2624109 | Certificate of Analysis | Jun 25, 2026 | F408902 |
| Punto di fusione (°C) | 256-260°C |
|---|
| 1. Jingtong Xia, Jiahao Zhou, Yanlong Liu, Na Yan, Xiaowen Hu, Lei Zhou, Qiaosheng Pu. (2023) Non-destructive distinction of single seed for Medicago sativa and Melilotus officinalis by capillary electrophoresis with laser-induced fluorescence detection. JOURNAL OF CHROMATOGRAPHY A, [PMID:37290349] [10.1016/j.chroma.2023.464116] |
| 2. Lijun Zheng, Hongjia Xu, Hanxin Zhang, Caihong Shi, Wei Zhou, Xiangrong Zhang. (2023) Nanostructured lipid carrier as a strategy for encapsulation of formononetin and perilla seed oil: In vitro characterization and stability studies. Food Bioscience, [PMID:] [10.1016/j.fbio.2023.102707] |
| 3. Lei He, Qian Hu, Liyang Wei, Xuliyang Ge, Ning Yu, Ying Chen. (2023) Unravelling dynamic changes in non-volatile and volatile metabolites of pulses during soaking: An integrated metabolomics approach. FOOD CHEMISTRY, [PMID:37141754] [10.1016/j.foodchem.2023.136231] |
| 4. Zili Guo, Yilin Liu, Jiani Xiang, Xianrui Liang. (2023) Mechanochemical preparation of red clover extract/β-cyclodextrin dispersion: Enhanced water solubility and activities in alleviating high-fat diet-induced lipid accumulation and gut microbiota dysbiosis in mice. FOOD CHEMISTRY, [PMID:37060670] [10.1016/j.foodchem.2023.136084] |
| 5. Jiahui Ji, Lianggong Zhao, Xiaohua Liu, Huifang Wu, Donghan Wang, Dan Liu, Xinyue Chen, Shilan Feng. (2022) Green synthesis, characterization of formononetin mediated AgNPs and its testing for formothion in typical fruit and vegetable samples. JOURNAL OF FOOD COMPOSITION AND ANALYSIS, [PMID:] [10.1016/j.jfca.2022.104572] |
| 6. Jie Zhao, Lin Huang, Renjie Li, Zhuangwei Zhang, Jin Chen, Hongjin Tang. (2022) Insights from multi-spectroscopic analysis and molecular modeling to understand the structure–affinity relationship and the interaction mechanism of flavonoids with gliadin. Food & Function, 13 (9): (5061-5074). [PMID:35404372] [10.1039/D1FO03816H] |
| 7. Jiahui Ji, Huifang Wu, Donghan Wang, Dan Liu, Xinyue Chen, Shilan Feng. (2022) Green synthesis, characterization of Radix Hedysari-mediated silver nanoparticles and their use for sensitive colorimetric detection of Pb2+ in the Yellow River medium. Analytical Methods, 14 (6): (643-651). [PMID:35080529] [10.1039/D1AY01852C] |
| 8. Hongjin Tang, Lin Huang, Dongsheng Zhao, Chunyong Sun, Ping Song. (2020) Interaction mechanism of flavonoids on bovine serum albumin: Insights from molecular property-binding affinity relationship. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, [PMID:32480277] [10.1016/j.saa.2020.118519] |
| 9. Jie Zhao, Lin Huang, Chunyong Sun, Dongsheng Zhao, Hongjin Tang. (2020) Studies on the structure-activity relationship and interaction mechanism of flavonoids and xanthine oxidase through enzyme kinetics, spectroscopy methods and molecular simulations. FOOD CHEMISTRY, [PMID:32330646] [10.1016/j.foodchem.2020.126807] |
| 10. Haifeng Li, Shuang Wang, Fan Cui, Beibei Zhuo, Shuang Wang, Chenrui Zhao, Weilu Liu. (2020) Sensitive and selective detection of puerarin based on the hybrid of reduced graphene oxide and molecularly imprinted polymer. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, [PMID:32145538] [10.1016/j.jpba.2020.113221] |
| 11. Li-Ting NIU, Gen-Gen LI, Hai-Feng LI, Fan CUI, Jing ZHANG, Ying HUANG, Kai CHEN, Jian-Fu ZHANG, Wen-Fei LI, Wei-Lu LIU. (2019) A Novel Genistein Electrochemical Sensor Based on Molecularly Imprinted Polycarbazole/Carboxylated Multiwalled Carbon Nanotubes Nanocomposite. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, [PMID:] [10.1016/S1872-2040(19)61176-X] |
| 12. Xiafen Xu, Xinhui Li, Xianrui Liang. (2017) Application of ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry in identification of three isoflavone glycosides and their corresponding metabolites. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 32 (3): (262-268). [PMID:29222826] [10.1002/rcm.8038] |
| 13. Song Wang, Xinnan Chang, Jing Li, Zuoqiao Shi, Guowen Li. (2025) Identification of chemical components and metabolites in rats after oral administration of Epimedium-Astragalus granule pair by liquid chromatography-high resolution mass spectrometry combined with diagnostic fragment ions and mass defect filtering. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, [PMID:40014893] [10.1016/j.jpba.2025.116768] |
| 14. Yong Jiang, Wenjun Ji, Ying Lu, Qin Wang, Linwei Chen. (2025) Integrating Plasma Metabolomics, Network Pharmacology, and Experimental Validation to Investigate the Action Mechanism of Qiangxin Lishui Prescription in Chronic Heart Failure. BIOMEDICAL CHROMATOGRAPHY, 39 (2): (e6065). [PMID:39748248] [10.1002/bmc.6065] |
| 15. Lin Cheng, Zhirui Du, Xueqiao Yan, Manju Che, Guoguo Zhi, Xiuying Ma, Funeng Geng, Baojie Li. (2025) Formononetin From Sophora flavescens Aiton Alleviates Atopic Dermatitis by Suppressing Neutrophil Extracellular Traps. PHYTOTHERAPY RESEARCH, [PMID:40635516] [10.1002/ptr.70020] |
| 16. Zhenghao Wu, Guqing Luo, Hongjie Li, Min Chen, Guangbo Wu, Qiang Fan, Chihao Zhang, Jiwei Yu, Jiayun Lin, Jinbo Zhao, Xiaoliang Qi, Haizhong Huo, Meng Luo, Lei Zheng. (2025) Therapeutic potential of formononetin in cirrhotic portal hypertension: modulating hepatic fibrosis, macrophage polarization, and lymphangiogenesis. Frontiers in Immunology, [PMID:40852727] [10.3389/fimmu.2025.1571007] |
| 17. Jinwu Zhang, Bo Zhang, Shiqi Huang, Jianhao Deng, Yiying Liang, Jiakang He, Tingjun Hu, Liji Xie, Hailan Chen, Meiling Yu. (2025) Flavonoids of S. suberectus Dunn Regulate Cyclophosphamide-Induced Immunosuppression Through NF-κB Pathways. Veterinary Sciences, 12 (8): (762). [PMID:40872712] [10.3390/vetsci12080762] |
| 18. Shuai Mao, Yubin Liang, Zikang Chen, Lei Wang, Quanfu Chen, Zhuting Fang, Qifan Zheng, Wen Ma, Hanping Zhang, Zhiqiang Yu, Ling Yu. (2025) Adaptive bioactivable nanosystems for synergistic myocardial infarction therapy using traditional pharmaceutics. Bioactive Materials, [PMID:40988940] [10.1016/j.bioactmat.2025.08.041] |
| 19. Zhirui Du, Xueqiao Yan, Lin Cheng, Haiyu Shen, Guoguo Zhi, Baojie Li, Qian Yu. (2025) Luteolin attenuates experimental dry eye disease via dual modulation of macrophage JAK-STAT signaling and corneal epithelial MAPK pathways. PHYTOMEDICINE, [PMID:41005053] [10.1016/j.phymed.2025.157300] |
| 20. Chun Li, Yuxin Xie, Shaoyu Hu, Hong Yu, Yunke Xu, Hongping Shen, Yuan Yuan, Long Gu, Bangming Pu. (2023) Identification of formononetin as the active compound of CR-SR in hepatocellular carcinoma treatment: An integrated approach combining network pharmacology and weighted gene co-expression networks. Chemical Biology & Drug Design, 103 (1): (e14363). [PMID:37793997] [10.1111/cbdd.14363] |
| 21. Lei Qian, Hu Xu, Ruqiang Yuan, Weijing Yun, Yufang Ma. (2023) Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction. BIOMEDICINE & PHARMACOTHERAPY, [PMID:38070245] [10.1016/j.biopha.2023.116000] |
| 22. Yang Jun, Sha Xiaowei, Wu Di, Wu Bo, Pan Xiaohua, Pan Li-Long, Gu Yuanlong, Dong Xiaoliang. (2023) Formononetin alleviates acute pancreatitis by reducing oxidative stress and modulating intestinal barrier. Chinese Medicine, 18 (1): (1-12). [PMID:37370098] [10.1186/s13020-023-00773-1] |
| 23. Boshu Ouyang, Lingling Deng, Fangyong Yang, Hanlin Shi, Na Wang, Weifeng Tang, Xi Huang, Yaolong Zhou, Hang Yu, Ying Wei, Jingcheng Dong. (2023) Albumin-based formononetin nanomedicines for lung injury and fibrosis therapy via blocking macrophage pyroptosis. Materials Today Bio, [PMID:37214555] [10.1016/j.mtbio.2023.100643] |
| 24. Cui Zhanding, Wang Qian, Li Dengliang, Zhao Shihui, Zhang Qian, Tan Yuxin, Gong Qianwen, Liu Ting, Shao Jiang, Zhang Shuang, Huang Hailong, Wang Junzheng, Pei Zhihua, Dong Hao, Wang Kai, Hu Guixue, Li Zhiyong. (2021) Icariin, Formononetin and Caffeic Acid Phenethyl Ester Inhibit Feline Calicivirus Replication In Vitro. ARCHIVES OF VIROLOGY, 166 (9): (2443-2450). [PMID:34173062] [10.1007/s00705-021-05107-w] |
| 25. Dacheng Wu, Keyan Wu, Qingtian Zhu, Weiming Xiao, Qing Shan, Zhigang Yan, Jian Wu, Bin Deng, Yan Xue, Weijuan Gong, Guotao Lu, Yanbing Ding. (2018) Formononetin Administration Ameliorates Dextran Sulfate Sodium-Induced Acute Colitis by Inhibiting NLRP3 Inflammasome Signaling Pathway. MEDIATORS OF INFLAMMATION, [PMID:29507526] [10.1155/2018/3048532] |
| 26. Shuping Zhang, Zhenhua Liu, Jia Li, Qian Liu, Yongqing Zhang, Gaobin Pu. (2025) Cloning and functional verification of Geraniol-10-Hydroxylase gene in Lonicera japonica. PeerJ, [PMID:39822969] [10.7717/peerj.18832] |
| 27. Zhanding Cui, Jinlong Liu, Jinlong Wang, Jing Zhang, Yimei Cao, Kun Li, Zhixun Zhao, Hong Yuan, Xingwen Bai, Xueqing Ma, Pinghua Li, Yuanfang Fu, Huifang Bao, Dong Li, Qiang Zhang, Zaixin Liu, Kai Li, Tong Xu, Zengjun Lu. (2025) Formononetin and mizoribine inhibit Porcine Reproductive and Respiratory Syndrome Virus replication in vitro. Frontiers in Nutrition, [PMID:40196742] [10.3389/fnut.2024.1501685] |
| 28. Haolin Zhang, Jingjing Zhang, Hui Cao, Petra Högger, Randolph Arroo, Mohamed A. Farag, Avi Shpigelman, Jianbo Xiao, Chunlin Li. (2025) Amination of Flavonoids Possessing a Pyrogallol Group in Cell Culture Medium at 37°C. Food Frontiers, 6 (2): (1058-1078). [PMID:] [10.1002/fft2.70002] |
| 29. Jie Yuan, Chongyong Gao, Wang Xin, Fanlin Meng, Hong Zhang. (2025) Screening and validation of 3’-Methoxydaidzein as a therapeutic agent in ulcerative colitis based on disulfidptosis-associated molecular clusters. PLoS One, 20 (6): (e0324586). [PMID:40478904] [10.1371/journal.pone.0324586] |
| 30. Zhanbo Xiong, Xiaoyin Zhang, Shiqi Zhang, Kexin Li, Yuan Gao, Ying Bu, Nan Zheng, Shengguo Zhao, Jiaqi Wang. (2025) Effects of red clover isoflavone on lactation performance, milk quality and nitrogen metabolism in dairy cows. Animal Nutrition, [PMID:40896491] [10.1016/j.aninu.2025.03.010] |
| 31. Jianwei Luo, Yingsi Huang, Xinghai Huang, Jinyun Cai, Haiyi Zhong. (2025) Closed-Loop Utilization of Sophora tonkinensis Gagnep.: Nanocellulose from Non-Medicinal Parts into Magnetic Imprinted Polymers for Targeted Quercetin Separation of Medicinal Parts. ACS Sustainable Chemistry & Engineering, [PMID:] [10.1021/acssuschemeng.5c05620] |
| 32. Yunshi Zhi, Li Pan, Wenjun Xiong, Thomas Chuen Lam, Huihui Xiao, Chi Wai Do. (2026) Ocular and Systemic Pharmacokinetics of Baicalein and Baicalin After Intravitreal Injection and Oral Administration in Mice. Pharmaceutics, 18 (2): (243). [PMID:41754984] [10.3390/pharmaceutics18020243] |
| 33. Xiaotian Gao, Xiaoniao Chen, Luying Qiao, Yingtao Zhang, Xiaoling Liu, Sifan Yang, Yan Zheng, Xun Chen, Xu Wang, Ran Liu, Zheyi Dong, Qian Wang, Quan Hong. (2026) Formononetin attenuates early diabetic retinal and renal microvascular injury associated with EGR1/VEGF-related endothelial responses. JOURNAL OF ETHNOPHARMACOLOGY, [PMID:] [10.1016/j.jep.2026.122294] |
