FPFT-2216 - 10mM in DMSO , CAS No.2367619-87-0

CAS: 2367619-87-0 Cat. No.: F656278 Formula: C₁₂H₁₂N₄O₃S Peso molecolare: 292.31

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Panoramica

FPFT-2216, a “molecular glue” compound, degrades phosphodiesterase 6D ( PDE6D ), zinc finger transcription factors Ikaros ( IKZF1 ), Aiolos ( IKZF3 ), and casein kinase 1α ( CK1α ). FPFT-2216 can be used for the research of cancer and inflammatory disease

In Vitro

FPFT-2216 (1 μM; 5 hours) is able to degrade PDE6D, in addition to its known targets IKZF1, IKZF3, and CK1α in MOLT4 cells. FPFT-2216 (1 μM; 0 h, 2 h, 4 h, 6 h, 16 h, 24 h) shows complete degradation of PDE6D within 2 h, and the degradation of PDE6D persists for at least 24 h in MOLT4 cells. FPFT-2216 (0 nM, 1.6 nM, 8 nM, 40 nM, 200 nM, 1 μM; 4 h) exhibits over 50% degradation of PDE6D at a dose of 8 nM, while maximum degradation of PDE6D along with IKZF1, IKZF3, and CK1α at a dose of 200 nM in MOLT4 cells. FPFT-2216 does not impede the growth of KRAS G12C -dependent MIA PaCa-2 cells. FPFT-2216 (10, 20, 40 µM; 14 or 24 h) highly up-regulates the production of IL-2 although it is less potent than that of Pomalidomide in Naive CD4 + T cells. FPFT-2216 (10 µM; 14 or 24 h) degrades IKZF1 and CK-1α among ubiquitin–proteasomal degradative substrates of immunomodulatory drugs (IMiDs) in Naive CD4 + T cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: MOLT4 cells Concentration: 1 μM Incubation Time: 0 h, 2 h, 4 h, 6 h, 16 h, 24 h Result: Showed complete degradation of PDE6D within 2 h, and the degradation of PDE6D persisted for at least 24 h. Western Blot AnalysisCell Line: MOLT4 cells Concentration: 0 nM, 1.6 nM, 8 nM, 40 nM, 200 nM, 1 μM Incubation Time: 4 h Result: Exhibited over 50% degradation of PDE6D at a dose of 8 nM, while maximum degradation of PDE6D along with IKZF1, IKZF3, and CK1α at a dose of 200 nM.

In Vivo

FPFT-2216 (30 mg/kg; p.o. or i.p.) induces significant degradation of CK-1α, and IKZF1 in CRBN I391V mice. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: CRBN I391V miceDosage: 30 mg/kg (solubilized in 0.5% carboxymethylcellulose/sodium and 0.25% Tween 80) Administration: p.o. or i.p. Result: Induced significant degradation of CK-1α, and IKZF1.

IC50& Target:PDE6D CK1α IKZF1 IKZF3

Specifications

Specifiche e purezza

10mM in DMSO

Meccanismi biochimici e fisiologici

FPFT-2216, a “molecular glue” compound, degrades phosphodiesterase 6D ( PDE6D ), zinc finger transcription factors Ikaros ( IKZF1 ), Aiolos ( IKZF3 ), and casein kinase 1α ( CK1α ). FPFT-2216 can be used for the research of cancer and inflammatory disease

Condizioni di conservazione di stoccaggio

Store at -80°C

Spedito in

Dry ice packs + Cold packs

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Tipo di azione

INHIBITOR

Nomi e identificatori

• Peso molecolare: 292.31

Domande frequenti

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2367619-87-0, the molecular formula is C12H12N4O3S, and the molecular weight is 292.31 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.