Gamborg B5 Vitamin Solution - BioReagent

Cat. No.: G1528866

GRADE & PURITY BioReagent

Storage

Store at 2-8°C

Shipped In

Wet ice

Panoramica

Gamborg B5 Vitamin Solution is an important commonly-used additive in plant tissue culture. Its formula is derived from research published by Gamborg et al. in 1968.

Specifications

Specifiche e purezza

BioReagent

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

BioReagent

Product Line

Prodotti biologici

Application Protocols

Manufacturer‑tested applications and specific use instructions are not provided for this SKU.

General example workflow (literature guidance; adjust to your SOP and the product label)

• Prepare Gamborg B5 basal medium with carbon source; adjust pH as required.
• Autoclave the basal medium.
• Under aseptic conditions, cool to ~45–55°C.
• Add Gamborg B5 Vitamin Solution to reach the desired working concentration per the product label (concentration not specified here). Swirl gently to mix.
• If needed, add sterile plant growth regulators and antibiotics.
• Dispense into plates or culture vessels and allow to solidify (if gelled).
• Inoculate with explants or cells; incubate under species‑appropriate light/temperature.

Quality control checkpoints

• Visual inspection for precipitation or turbidity after addition.
• Optional sterility test of final medium batch.

For exact dilution factors, component concentrations, and validated applications, consult the CoA/Spec Sheet for SKU G1528866.

Biological Roles

The components commonly included in Gamborg B5 vitamin formulations support essential cellular metabolism in plant tissues (general literature; composition for this SKU is not specified).

• Thiamine (vitamin B1, typically as thiamine HCl): Coenzyme precursor for thiamine pyrophosphate (TPP), required by pyruvate dehydrogenase, α‑ketoglutarate dehydrogenase, and transketolase. Supports central carbon metabolism, the TCA cycle linkage, and the pentose phosphate pathway.
• Pyridoxine (vitamin B6, typically as pyridoxine HCl): Precursor of pyridoxal‑5′‑phosphate (PLP), a versatile coenzyme in amino acid metabolism, including transamination, decarboxylation, and racemization; contributes to synthesis of phytohormones and stress‑related metabolites.
• Nicotinic acid (niacin): Precursor to NAD+/NADP+, central redox cofactors in catabolic/anabolic reactions; critical for oxidative balance and biosynthetic pathways (fatty acids, nucleotides).
• Myo‑inositol (included in some B5 vitamin mixes): Functions in cell wall biosynthesis (as a precursor to pectic polysaccharides), membrane signaling (phosphoinositides), and osmoprotection; can improve cell division and morphogenesis in vitro.

Role in tissue culture (general)

• Exogenous vitamin supply compensates for limited biosynthetic capacity of dedifferentiated cells and stressed explants, enhancing callus initiation, shoot/root regeneration, and suspension growth.
• Vitamin balance can modulate responsiveness to auxins/cytokinins, influencing organogenic vs. embryogenic pathways.

Note: For experimental design and reporting, cite the exact vitamin composition and concentrations actually used (from CoA/label) to enable reproducibility.

Buffer Applications

This product is not a buffering reagent and is not typically used to control pH in solutions.

• In plant tissue culture, medium pH is commonly adjusted (literature: often ~5.7) with acid/base prior to sterilization, using the basal salts’ inherent buffering capacity. Gamborg B5 Vitamin Solution is added for nutritional support, not for buffering.
• For buffering needs in plant culture, consider Good’s buffers (e.g., MES) as appropriate, verifying compatibility with your species and protocol.

Green Alternatives

Context

• Vitamins for plant tissue culture are inherently water-based and used at low concentrations, resulting in a comparatively small environmental footprint relative to organic solvents or hazardous reagents.

Greener practice considerations (general)

• Ready-to-use solutions vs. dry blends: Pre-made aqueous vitamin solutions can reduce dust exposure, minimize weighing errors, and lower wastage from out‑of‑spec media batches. Tradeoff: refrigerated storage and cold-chain shipping (wet ice) add energy use.
• Process efficiency: Filter-sterilizing concentrated vitamin stocks and aliquoting reduces repeated container waste and preserves product integrity, lowering discard rates.
• Packaging: Choose appropriate pack sizes to match throughput; this reduces expired materials and container disposal.
• Waste management: Aqueous vitamin solutions typically produce nonhazardous waste; confirm classification per local regulations and avoid unnecessary solvent use in cleanup.

Comparison (literature, illustrative)

• B5 vitamins vs. MS vitamins or custom mixes: All are aqueous and similar in EHS profile. Selection for "greenness" hinges on supply chain, packaging, and storage energy rather than intrinsic chemical hazard.

Actionable tips

• Consolidate cold shipments to reduce environmental impact.
• Store at 2–8°C as specified to extend shelf life and decrease product loss.
• Use light-resistant secondary containment to reduce degradation-driven waste.

Pharmaceutical Uses

Not applicable. This product is intended for laboratory research use only and is not manufactured under GMP for clinical or pharmaceutical applications.

• No pharmacopeial status is claimed for SKU G1528866. Do not use for human or animal therapeutic, diagnostic, or processing applications.
• In the bioprocessing context, plant tissue culture vitamins are used for in vitro plant systems and are not typical excipients in human/animal formulations.

Physical Properties

Item-specific properties (as supplied)

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration of each vitamin: Not specified for this item; refer to CoA/Spec Sheet.
• pH, conductivity, osmolality: Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, UV cutoff: Not specified for this item; refer to CoA/Spec Sheet.

General characteristics (literature/typical for aqueous vitamin solutions; not product specifications)

• Matrix: Aqueous, sterile-filtered solutions are commonly used to preserve vitamin integrity and enable aseptic addition to autoclaved media.
• Solubility: Components are water‑soluble (thiamine HCl, pyridoxine HCl, nicotinic acid; myo‑inositol is highly soluble in water). Practically insoluble in nonpolar solvents.
• Stability considerations: Water‑soluble vitamins can be heat- and light‑sensitive; solutions are typically stored cold and protected from light to minimize degradation and oxidation.

Practical notes

• Avoid prolonged exposure to elevated temperatures and intense light during handling.
• Prepare media by adding the vitamin solution aseptically to cooled (≈45–55°C) sterilized basal salt/agar solutions to reduce thermal decomposition (general practice; verify with your SOP and product label).

All numeric values for this specific item are not provided; consult the CoA/Spec Sheet for definitive physical information.

Quality and Grades

• Grade: BioReagent (item-specific)

  • Meaning: BioReagent grade materials are intended for biological and biochemical research applications, emphasizing low levels of contaminants that could interfere with cell/tissue culture or enzymatic processes. They are not produced under GMP for clinical use.
  • Implications for plant tissue culture: Consistency in vitamin content and low bioburden are critical for reproducible callus formation, organogenesis, and suspension cultures.

• Stabilizers/Preservatives: Not specified for this item; refer to CoA/Spec Sheet.

• Sterility: Not specified for this item; refer to CoA/Spec Sheet. Many vitamin solutions are sterile-filtered; verify before aseptic addition to media.

• UV absorbance, heavy metals, endotoxin/bioburden: Not specified for this item; refer to CoA/Spec Sheet.

Best practices for quality assurance

• Use within the recommended shelf-life and store at 2–8°C as specified for SKU G1528866.
• Minimize repeated warming/cooling cycles; aliquot on first use if frequent access is expected to limit contamination and degradation.
• For regulated workflows: retain lot-specific CoAs and perform incoming QC checks (e.g., sterility, pH impact on media, a defined growth response assay with a reference plant line).

Note: If your application is highly sensitive (e.g., hormone‑response assays), consider verifying vitamin concentrations via analytical methods (HPLC/UV or LC‑MS) against certified standards.

Reaction and Applications

Primary application: plant tissue culture (research use only)

• Use as the vitamin supplement in Gamborg B5-based media for callus induction, organogenesis, somatic embryogenesis, and maintenance of plant cell suspensions.
• Suitable for explant culture workflows, including leaf, hypocotyl, cotyledon, and root segments, in combination with appropriate basal salts (B5), carbon source (commonly sucrose), gelling agent (agar/gelrite), and plant growth regulators (auxins/cytokinins) tailored to the species and objective.

Practical tips (general literature guidance)

• Sterilization: Do not autoclave vitamins unless explicitly permitted; instead, aseptically add the vitamin solution to the medium after autoclaving and cooling to ~45–55°C.
• Light/temperature sensitivity: Thiamine and pyridoxine can degrade with heat and light; minimize exposure during preparation and storage.
• Optimization: Vitamin concentration can influence morphogenesis and growth rate. Start with the supplier’s recommended dilution (not specified here; consult label) and optimize empirically for the plant line.
• Compatibility: Vitamins are typically compatible with common plant hormones (IAA, NAA, 2,4‑D, BAP, kinetin). Monitor pH after addition (target medium pH is often ~5.7, literature) and adjust before gelling/sterilization as required by your SOP.
• Cell suspensions: In liquid B5 media, adequate aeration/shaking and frequent subculture help prevent vitamin depletion and maintain cell vitality.

Note: Manufacturer‑provided specific applications are not listed for this SKU; the above describes standard uses of Gamborg B5 vitamin supplements in plant biotechnology.

Reaction Conditions

While not used in chemical reactions, practical conditions for media preparation with Gamborg B5 Vitamin Solution (general literature guidance) include:

• Basal medium: Prepare Gamborg B5 basal salts with carbon source (commonly 20–30 g/L sucrose) and gelling agent if preparing solid media.
• pH: Adjust medium to the target pH (literature often ~5.7) before sterilization, unless your SOP specifies otherwise.
• Sterilization: Autoclave basal salts/agar solution. Do not autoclave the vitamin solution unless explicitly indicated by the supplier.
• Addition of vitamins: Cool sterilized medium to approximately 45–55°C; add the vitamin solution aseptically to achieve the desired final concentration (not specified here; refer to product label/CoA). Mix gently to avoid bubble entrainment.
• Plant growth regulators: Add sterile-filtered hormones (e.g., auxins/cytokinins) as required for the application after cooling, similar to vitamins.
• Dispensing and solidification: Pour plates or dispense into culture vessels under aseptic conditions; allow to solidify (if gelled) and equilibrate before introducing explants or cells.
• Storage of prepared medium: Store wrapped plates or sealed vessels at 2–8°C, protected from light; use within the period validated by your lab to limit vitamin degradation and medium desiccation.

Note: Specific times, temperatures, and concentrations must follow your institutional SOPs and the product’s CoA/label.

Safety and Handling

GHS and hazard communication (item-specific)

• Signal word: Not specified for this item; refer to SDS.
• Hazard statements (H-codes): Not specified for this item; refer to SDS.
• GHS classification and pictograms: Not specified for this item; refer to SDS.

General safety guidance (literature/standard lab practice)

• While vitamin solutions are generally of low acute hazard, always handle under appropriate aseptic conditions to prevent contamination of tissue cultures.
• Personal protective equipment (PPE): lab coat, safety glasses, and nitrile gloves. Use in a clean environment (biosafety cabinet recommended for culture work).
• Avoid ingestion, inhalation of aerosols/sprays, and contact with skin/eyes. Wash thoroughly after handling.
• Incompatibilities: Strong oxidants may degrade vitamins; avoid mixing with concentrated acids/bases or reactive metals. Do not autoclave the vitamin solution unless specifically indicated by the product documentation.
• First aid (overview; consult SDS for details):
  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; seek medical attention if irritation persists.
  • Inhalation: Move to fresh air.
  • Ingestion: Rinse mouth; seek medical advice.
• Spill response: Absorb with inert material, clean with water and detergent; dispose according to institutional and local regulations.

Always consult the SDS for SKU G1528866 for authoritative hazard, exposure controls, and disposal requirements.

Solvent Selection

This product is an aqueous vitamin solution used as an additive for plant tissue culture media; conventional "solvent selection" is not typically applicable.

Practical guidance

• Matrix compatibility: Designed for water-based media (e.g., Gamborg B5 basal salts, sucrose, agar/gel agents). Fully miscible with aqueous solutions.
• Sterilization strategy: Add to autoclaved media after cooling to approximately 45–55°C to limit thermal degradation (general practice; verify with product instructions). Aseptically filter the vitamin solution only if recommended by the supplier and your SOP.
• Avoid organic co-solvents: The presence of alcohols or strong organic solvents in culture media can harm plant tissues; keep additions strictly aqueous unless your protocol validates tolerability.

Comparison (literature context)

• Gamborg B5 vitamins vs. MS vitamins: Both support plant cell growth; B5 vitamins typically provide higher thiamine levels, which can benefit certain legumes and cell suspensions. Selection is empirical and species/line dependent.

For detailed solvent or media compatibility beyond water-based systems, consult your specific tissue culture protocol and the product CoA.

Storage and Reconstitution

Item-specific storage and shipping

• Storage conditions: Store at 2–8°C (per Product Data for SKU G1528866). Protect from light to minimize vitamin degradation (general recommendation).
• Shipping: Shipped on wet ice (per Product Data).

Stability and handling (general guidance)

• Keep container tightly closed. Minimize time at room temperature; return to 2–8°C promptly after use.
• If sterility is critical, work in a biosafety cabinet. Clean septa/caps with 70% ethanol before access.
• Aliquot upon first opening to reduce contamination risk and repeated temperature cycling. Use sterile, low‑binding polypropylene tubes.
• Avoid freeze–thaw unless the label explicitly allows freezing; repeated freeze–thaw can degrade some vitamins.

Reconstitution/use

• This product is supplied as a solution; no reconstitution is required. The working dilution and target final concentrations in media are not specified for this item; refer to the product label/CoA.
• If precipitates form during cold storage, allow to equilibrate at room temperature briefly and mix gently to redissolve. Discard if insoluble matter or contamination persists.

Shelf life and documentation

• Follow the expiration date on the label. Record lot numbers and opening dates to support traceability and method reproducibility.

Structure and Identity

Gamborg B5 Vitamin Solution is a multicomponent, aqueous mixture formulated for plant tissue culture. It is not a single, well-defined molecule; therefore conventional single-compound identifiers do not apply to the product as a whole.

• Item-specific identifiers:
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not applicable (mixture).
  • SMILES: Not applicable (mixture).
  • Molecular formula: Not applicable (mixture of several vitamins).
  • Molecular weight: Not applicable (mixture).
• Structural features (general, literature):
  • Typical Gamborg B5 vitamin formulations (literature) include water‑soluble vitamins such as thiamine HCl (vitamin B1), nicotinic acid (niacin), pyridoxine HCl (vitamin B6), and, in some variants, myo‑inositol. Each component possesses distinct functional groups (e.g., pyrimidine–thiazolium for thiamine; pyridine carboxylic acid for niacin; substituted pyridine alcohol for pyridoxine; cyclitol polyol for myo‑inositol).
  • These components are fully ionizable or highly polar, designed for aqueous media compatibility.

Notes

• The exact component list, concentrations, and identities for SKU G1528866 are not specified; consult the product label or CoA for definitive composition.
• For mixture products, reporting a single MF/MW/SMILES is chemically invalid; component-level details must be used if needed for regulatory or analytical purposes.

Synthetic Utility

Not applicable as a reagent for chemical synthesis.

• Gamborg B5 Vitamin Solution is formulated for biological media supplementation and does not serve as a building block, catalyst, or solvent in organic synthesis.
• If component‑level chemistry is required (e.g., analytical standards of thiamine or pyridoxine), procure pure compounds rather than a mixed vitamin solution.

Target Specificity

Not applicable. This product is not an antibody, enzyme inhibitor, or targeted probe. It is a general nutrient supplement for plant tissue culture media.