Gankyrin Mouse mAb - Anticorpo primario, specific to Gankyrin, Mouse IgG1

Cat. No.: Ab104742

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Application

WB

Host species

Mouse

Reactive species

Maiale, Umano

Isotype

Mouse IgG1

Clone Number

C5

Conjugation

Unconjugated

Panoramica

Mouse anti Human Gankyrin Antibody, Monoclonal (C5), could be used for WB and so on.
Application:
WB: 0.01-2 µg/mL
Protein Function:
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.

Specifications

Product Name

Gankyrin Mouse mAb - Anticorpo primario, specific to Gankyrin, Mouse IgG1

Sinonimi

26S proteasome non-ATPase regulatory subunit 10 antibody | 26S proteasome regulatory subunit p28 antibody | Ankyrin repeat protein antibody | dJ889N15.2 antibody | Gankyrin antibody | Hepatocellular carcinoma-associated protein p28 II antibody | p28 antib

Specifiche e purezza

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Specie ospite

Mouse

Specificità

Gankyrin

Immunogeno

Recombinant PSMD10 expressed in E.coli (1-226).

Controllo positivo

WB: A549, MCF7 and HeLa cell lysates.

Coniugazione

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified + Protein G purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Gankyrin Mouse mAb (Ab104742)

Gankyrin Mouse mAb (Ab104742) - Western Blot
All lanes: Gankyrin Mouse mAb (Ab104742) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 25 kDa
Exposure time: 4.8 s

Applicazione

Applicazione

Dilution info

WB

0.01-2 µg/mL

Obiettivi associati (umani)

PSMD10 Tbio 26S proteasome non-ATPase regulatory subunit 10 (0 Activities)

Discover Target: PSMD10

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Gankyrin Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.