GLS2 Mouse mAb - Primary antibody, specific to GLS2

Cat. No.: Ab105528

GRADE & PURITY See COA

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse

Isotype

Mouse IgG1

Clone Number

H58S879

Conjugation

Unconjugated

Overview

Mouse anti Human GLS2 Antibody, Monoclonal (H58S879), could be used for WB, IHC and so on.

Specifications

Product Name

GLS2 Mouse mAb - Primary antibody, specific to GLS2

Synonyms

breast cell glutaminase antibody | GA antibody | GLS antibody | Gls2 antibody | GLSL_HUMAN antibody | glutaminase 2 (liver mitochondrial) antibody | glutaminase 2 antibody | glutaminase GA antibody | glutaminase I antibody | Glutaminase liver isoform anti

Specifications & Purity

See COA

Biochemical and Physiological Mechanisms

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH

Host species

Mouse

Specificity

GLS2

Immunogen

Recombinant protein of human GLS2

Positive Control

WB: Human brain, Mouse liver lysates. IHC: Human kidney tissue.

Conjugation

Unconjugated

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: GLS2 Mouse mAb (Ab105528)

GLS2 Mouse mAb (Ab105528) - Western Blot 

All lanes: GLS2 Mouse mAb (Ab105528) at 1/500 dilution Samples: Lysates at 20 µg per lane 

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution 

Predicted band size: 66 kDa 

Observed band size: 66 kDa 

Exposure time: 60.0 s

IHC: GLS2 Mouse mAb (Ab105528)

GLS2 Mouse mAb (Ab105528) - IHC Immunohistochemical analysis of GLS2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GLS2 Mouse mAb (Ab105528) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Application

• Application: Dilution info
• IHC:

  1/50-1/200;

• WB:

  1/500-1/1000 ;

Associated Targets(Human)

GLS2 Tchem Glutaminase liver isoform, mitochondrial (0 Activities)

Discover Target: GLS2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)