GLS2 Mouse mAb - Anticorpo primario, specific to GLS2

Cat. No.: Ab105528

GRADE & PURITY See COA

Application

IHC, WB

Host species

Mouse

Reactive species

Mouse, Umano

Isotype

Mouse IgG1

Clone Number

H58S879

Conjugation

Unconjugated

Panoramica

Mouse anti Human GLS2 Antibody, Monoclonal (H58S879), could be used for WB, IHC and so on.

Specifications

Product Name

GLS2 Mouse mAb - Anticorpo primario, specific to GLS2

Sinonimi

breast cell glutaminase antibody | GA antibody | GLS antibody | Gls2 antibody | GLSL_HUMAN antibody | glutaminase 2 (liver mitochondrial) antibody | glutaminase 2 antibody | glutaminase GA antibody | glutaminase I antibody | Glutaminase liver isoform anti

Specifiche e purezza

See COA

Meccanismi biochimici e fisiologici

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH

Specie ospite

Mouse

Specificità

GLS2

Immunogeno

Recombinant protein of human GLS2

Controllo positivo

WB: Human brain, Mouse liver lysates. IHC: Human kidney tissue.

Coniugazione

Unconjugated

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: GLS2 Mouse mAb (Ab105528)

GLS2 Mouse mAb (Ab105528) - Western Blot 

All lanes: GLS2 Mouse mAb (Ab105528) at 1/500 dilution Samples: Lysates at 20 µg per lane 

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution 

Predicted band size: 66 kDa 

Observed band size: 66 kDa 

Exposure time: 60.0 s

IHC: GLS2 Mouse mAb (Ab105528)

GLS2 Mouse mAb (Ab105528) - IHC Immunohistochemical analysis of GLS2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GLS2 Mouse mAb (Ab105528) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Applicazione

• Applicazione: Dilution info
• IHC:

  1/50-1/200;

• WB:

  1/500-1/1000 ;

Obiettivi associati (umani)

GLS2 Tchem Glutaminase liver isoform, mitochondrial (0 Activities)

Discover Target: GLS2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GLS2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.