GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Myrosinase catalyzes the hydrolysis of glucosinolates to produce glucose. The glucose is then sequentially catalyzed by hexokinase (HK) and glucose‑6‑phosphate dehydrogenase (G6PDH) to generate 6‑phosphogluconate, with concomitant reduction of NADP⁺ to NADPH. The NADPH, via electron transfer mediated by 1‑mPMS, reduces WST‑1 to formazan, a product that exhibits a characteristic absorption peak at 450 nm. The glucosinolate content can be quantitatively determined by monitoring the change in absorbance.
Specifications
Specifiche e purezza
BioReagent
Grado
BioReagent
Detection instrument
Microplate reader
Detection wavelength
450nm
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Store at 2-8°C
Spedito in
Wet ice
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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